Integration of multi-oscillator circadian clock: in vivo and ex vivo imaging of clock gene expression
| Project/Area Number |
21390064
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Hokkaido University |
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Principal Investigator |
HONMA Sato 北海道大学, 大学院・医学研究科, 特任教授 (20142713)
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| Co-Investigator(Kenkyū-buntansha) |
吉川 朋子 (仲村 朋子) 北海道大学, 大学院・医学研究科, 特任助教 (30451397)
西出 真也 北海道大学, 大学院・医学研究科, 助教 (40451398)
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| Co-Investigator(Renkei-kenkyūsha) |
HAMADA Toshiyuki 北海道大学, 大学院・医学研究科, 特任講師 (20360208)
NAKAMURA Tomoko (YOSHIKAWA Tomoko) 北海道大学, 大学院・医学研究科, 特任助教 (30451397)
NISHIDE Shin-ya 北海道大学, 大学院・医学研究科, 助教 (40451398)
ENOKI Ryosuke 北海道大学, 大学院・医学研究科, 助教 (00528341)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2009: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Keywords | 生体リズム / 時計遺伝子 / イメージング / ルシフェラーゼ / 視交叉上核 / 生物リズム / 生物時計 / 発光イメージング / リズム同期 / 自発発火 |
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| Research Abstract |
We aimed to clarify mutual coupling mechanisms of oscillator cells in the central circadian clock, the suprachiasmatic nucleus (SCN). We developed in vivo monitoring systems for clock gene expression from the SCN of unanesthetized and unrestrained animals and a long-term and real-time ex vivo monitoring systems for intracellular Ca++ levels from the entire neurons of cultured SCN slices. We demonstrated that all SCN neurons exhibit robust circadian rhythms in Ca++ levels which are topologically specific and the coupling between the pacemakers in the dorsal and ventral SCN depends on synaptic interaction. We further demonstrated that clock gene Cry is essential for the development of oscillator cell network in the SCN for the coherent rhythm expression which takes place around weaning period.
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Report
(4 results)
Research Products
(90 results)
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[Journal Article] Regulation of basic helix-loop-helix transcription factors Dec1 and Dec2 by RORα and their roles in adipogenesis2012
Author(s)
Ozaki N, Noshiro M, Kawamoto T, Nakashima A, Honda K, Fukuzaki-Dohi U, Honma S, Fujimoto K, Tanimoto K, Tanne K and Kato, Y
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Journal Title
Genes Cells
Volume: 17
Pages: 109-21
Related Report
Peer Reviewed
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[Journal Article] Self-sustained circadian rhythm in cultured humanmononuclear cells isolated from peripheral blood2010
Author(s)
Ebisawa T, Numazawa K, Shimada H, IzutsubH, Sasaki T, Kato N, Tokunaga K, Akio Mori A, Honma K, Honma S, Shibata S
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Journal Title
Neurosci Res
Volume: 66
Issue: 2
Pages: 223-227
DOI
Related Report
Peer Reviewed
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[Journal Article] The liver X receptors (LXRα and LXRβ) are potent regulators for hepatic Dec1 expression2009
Author(s)
Noshiro M, Usui E, Kawamoto T, Sato F, Nakashima A, Ueshima T, Honda K, Fujimoto K, Honma S, Honma K, Makishima M and Kato Y
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Journal Title
Genes to Cells
Volume: 14
Pages: 29-40
Related Report
Peer Reviewed
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