Integrative analyses of circadian rhythms based on environment

• Project/Area Number: 21390066
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Hiroshima University
• Principal Investigator: TAKUMI Toru 広島大学, 大学院・医歯薬学総合研究科, 教授 (00222092)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 遺伝子 / 細胞・組織 / 環境 / 行動学 / 神経科学

Research Abstract

We use multiple high-throughput approaches including chromatin immunoprecipitation(ChIP)-based systematic analyses and DNA microarrays combined with bioinformatics, to generate genome-wide profiles of BMAL1 target genes. We reveal that, in addition to E-boxes, BMAL1 recognizes a consensus sequence CCAATG to elicit robust circadian expression. BMAL1 occupancy is found in more than 150 sites, including all known clock genes. Importantly, a significant proportion of BMAL1 targets include genes that encode central regulators of metabolic processes. The database generated in this study constitutes a useful resource to decipher the network of circadian gene control and its intimate links with several fundamental physiological functions

Research Products

• [Journal Article] REV-ERBa and the clock gene machinery in mouse peripheral tissues : a possible role as a synchronizing hing (2012)
  • Author(s): G. Mazzoccoli, Y. Cai, S. Liu, M. Francavilla, F. Giuliani, A. Piepoli, V. Pazienza, M. Vinciguerra, T. Yamamotoand T. Takumi
  • Journal Title: J. Biological Regulators & Homeostatic Agents, in press
  • Peer Reviewed
• [Journal Article] e clock gene machinery in mouse peripheral tissues : a possible role as a sync (2012)
  • Author(s): G.Mazzoccoli, et al
  • Journal Title: J.Biological Regulators & Homeostatic Agents Volume: (in press)
  • Peer Reviewed
• [Journal Article] The period of the somite segmentation clock is sensitive to Notch activity (2011)
  • Author(s): W. Kim, T. Matsui, M. Yamao, M. Ishibashi, K. Tamada, T. Takumi, K. Kohno, S. Oba, S. Ishii, Y. Sakumura and Y. Bessho
  • Journal Title: Mol. Biol. Cell Volume: 22 Pages: 3541-3549
  • Peer Reviewed
• [Journal Article] The period of the somite segmentation clock is sensitive to Notch activity (2011)
  • Author(s): W.Kim, et al
  • Journal Title: Mol.Biol.Cell Volume: 22 Issue: 18 Pages: 3541-3549
  • DOI: 10.1091/mbc.e11-02-0139
  • Peer Reviewed
• [Journal Article] Genome-wide profiling of the core clock protein BMAL1 targets reveals strict relationship with metabolism (2010)
  • Author(s): F. Hatanaka, C. Matsubara, J. Myung, T. Yoritaka, N. Kamimura, S. Tsutsumi, A. Kanai, Y. Suzuki, P. Sassone-Corsi, H. Aburatani, S. Sugano and T. Takumi
  • Journal Title: Mol. Cell. Biol Volume: 30 Pages: 5636-5648
  • Peer Reviewed
• [Journal Article] Decreased exploratory activity in the model mouse for 15q duplication syndrome (2010)
  • Author(s): K. Tamada, S. Tomonaga, F. Hatanaka, N. Nakai, K. Takao, T. Miyakawa, J. Nakatani and T. Takumi
  • Journal Title: implication in disturbance of serotonin signaling. PLoSONE Volume: 5
  • Peer Reviewed
• [Journal Article] 概日時計と気分障害 (2010)
  • Author(s): 内匠透
  • Journal Title: Clinical Neuroscience Volume: 28 Pages: 720-721
• [Journal Article] Decreased exploratory activity in a mouse model of 15q duplication syndrome ; implications for disturbance of serotonin signaling. (2010)
  • Author(s): Tamada, K., Tomonaga, S., Hatanaka, F., Nakai, N., Takao, K., Miyakawa, T., Nakatani, J., Takumi, T.
  • Journal Title: PLoS ONE Volume: 15
  • Peer Reviewed
• [Journal Article] Genome-wide profiling of the core clock protein BMAL1 targets reveals strict relationship with metabolism. (2010)
  • Author(s): Hatanaka, F., Matsubara, C., Myung, J., Yoritaka, T., Kamimura, N., Tsustumi, S., Kanai, A., Suzuki, Y., Sassone-Corsi, P., Aburatani, H., Sugano, S., Takumi, T.
  • Journal Title: Mol.Cell.Biol Volume: 30 Pages: 5636-5648
  • Peer Reviewed
• [Journal Article] Dual-color luciferase mouse directly demonstrates coupled expression of two clock genes. (2010)
  • Author(s): Noguchi, T., Michihata, T., Nakamura, W., Takumi, T., Shimizu, R., Yamamoto, M., Ikeda, M., Ohmiya, Y., Nakajima, Y.
  • Journal Title: Biochemistry Volume: 49 Pages: 8053-8061
  • Peer Reviewed
• [Journal Article] A humanoid mouse model of autism. (2010)
  • Author(s): Takumi, T.
  • Journal Title: Brain Dev Volume: 32 Pages: 753-758
  • NAID: 40016985988
  • Peer Reviewed
• [Journal Article] Mutations of optineurin in amyotrophic lateral sclerosis. (2010)
  • Author(s): Maruyama, H., Morino, H., Ito, H., Izumi, Y., Kato, H.Watanabe, Y., Kinoshita, Y., Kamada, M., Nodera, H., Suzuki, H., Komure, O., Matsuura, S., Kobatake, K., Morimoto, N., Abe, K., Suzuki, N., Aoki, M., Kawata, A., Hirai, T., Kato, T., Ogasawara, K., Hirano, A., Takumi, T., Kusaka, H., Hagiwara, K., Kaji, R., Kawakami, H.
  • Journal Title: Nature Volume: 465 Pages: 223-226
  • Peer Reviewed
• [Journal Article] Abnormal behavior in a chromosome-engineering mouse model for human 15q11-13 duplication seen in autism (2009)
  • Author(s): J. Nakatani, K. Tamada, F. Hatanaka, S. Ise, H. Ohta, K. Inoue, S. Tomonaga, Y. Watanabe, Y. J. Chung, R. Banerjee, K. Iwamoto, T. Kato, M. Okazawa, K. Yamauchi, K. Tanda, K. Takao, T. Miyakawa, A. Bradley and T. Takumi
  • Journal Title: Cell Volume: 137 Pages: 1235-1246
  • Peer Reviewed
• [Journal Article] Fezf1 is required for penetration of the basal lamina by olfactory axons to promote olfactory development (2009)
  • Author(s): Y. Watanabe, K. Inoue, A. Okuyama-Yamamoto, N. Nakai, J. Nakatani, K.-i. Nibu, N. Sato, Y. Iiboshi, K. Yusa, G. Kondoh, J. Takeda, T. Terashimaand T. Takumi
  • Journal Title: J. Comp. Neurol. Volume: 515 Pages: 565-584
  • Peer Reviewed
• [Journal Article] Time microscopy of circadian expression of circadian clock gene mRNA transcription (2009)
  • Author(s): R. B. Sothern, G. Cornelissen, T. Yamamoto, T. Takumi and F. Halberg
  • Journal Title: chronodiagnostic and chrono-the rapeutic implications. Clin. Ter Volume: 160
  • Peer Reviewed
• [Journal Article] Central and peripheral circadian clock genes, their statistical analysis for rhythms, and relationship to health and disease (2009)
  • Author(s): R. B. Sothern, T. Yamamoto, G. Cornelissen, T. Takumi and F. Halberg
  • Journal Title: Scripta Med Volume: 82 Pages: 133-163
  • Peer Reviewed
• [Journal Article] Robust food anticipatory activity in BMAL1-deficient mice (2009)
  • Author(s): J. S. Pendergast, W. Nakamura, R. C. Friday, T. Takumi and S. Yamazaki
  • Journal Title: PLoS ONE Volume: 4
  • Peer Reviewed
• [Journal Article] The resetting of the circadian rhythm by Prostaglandin J2 is distinctly phase -dependent (2009)
  • Author(s): Koinuma S, Yagita K, Fujioka A, Takashima N, Takumi T, Shigeyoshi Y
  • Journal Title: FEBS Letters 583 Pages: 413-418
  • Peer Reviewed
• [Journal Article] Robust food anticipatory activity in BMAL1-deficient mice (2009)
  • Author(s): Pendergast JS, Nakamura W, Friday RC, Hatanaka F, Takumi T, Yamazaki S
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Time microscopy of circadian expression of cardiac clock gene mRNA transcription : chronodiagnostic and chrono-therapeutic implications (2009)
  • Author(s): Sothern RB, Cornlissen G, Yamamoto T, Takumi T, Halberg F
  • Journal Title: Clinica Therapeutica 160
  • Peer Reviewed
• [Journal Article] Fezfl is required for penetration of the basal lamina by olfactory axons to promote olfactory development (2009)
  • Author(s): Watanabe Y, Inoue K, Okuyama-Yamamoto A, Nakai N, Nakatani J, Nibu K, Sato N, Iiboshi Y, Yusa K, Kondoh G, Takeda J, Terashima T.Takumi T.
  • Journal Title: The Journal of Comparative Neurology 515 Pages: 565-584
  • Peer Reviewed
• [Journal Article] A protein-protein interaction of stress-responsive myosin VI endowed to inhibit neural progenitor self-replication with RNA binding protein, TLS, in murine hippocampus (2009)
  • Author(s): Takarada T, Tamaki K, Takumi T, Ogura M, Ito Y, Nakamichi N.Yoneda Y.
  • Journal Title: Journal of Neurochemistry 110 Pages: 1457-1468
  • Peer Reviewed
• [Journal Article] Autonomous regulation of osteosarcoma cell invasiveness by Wnt5a/Ror2 signaling (2009)
  • Author(s): Enomoto M, Hayakawa S, Itsukushima S, Ren DY, Matsuo M, Tamada K, Oneyama C, Okada M, Takumi T, Nishita M, Minami Y
  • Journal Title: Oncogene 28 Pages: 3197-3208
  • Peer Reviewed
• [Journal Article] Abnormal behavior in a chromosome-engineered mouse model for human 15q11-13 duplication seen in autism (2009)
  • Author(s): Nakatani J, Tamada K, Hatanaka F, Ise S, Ohta H, Inoue K, Tomonaga S, Watanabe Y, Chung YJ, Banerjee R, Iwamoto K, Kato T, Okazawa M, Yamauchi K, Tanda K, Takao K, Miyakawa T.Bradley A.Takumi T
  • Journal Title: Cell 137 Pages: 1235-1246
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): NPO法人 脳の世紀推進会議編
  • Journal Title: こころの働きと病・覚醒剤(クバプロ) Pages: 75-96
• [Presentation] The phosphorylation of PERIOD2 via glycogen synthase kinase 3υhas the possibility of the regulator in the depression-like behavior (2011)
  • Author(s): Yousuke Yamawaki, Atsuko Takano, Toru Takumi
  • Organizer: The 32nd Naito Conference
  • Place of Presentation: Hokuto, Yamanashi
• [Presentation] Rhythm and the blues Worldsleep 2011 (2011)
  • Author(s): Toru Takumi
  • Place of Presentation: Kyoto
• [Presentation] Genome-wide profiling of the core clock protein BMAL1 targets reveals strict relationship with metabolism (2011)
  • Author(s): Fumiyuki Hatanaka, Chiaki Matsubara, Jihwan Myung, Takashi Yoritaka, NaokoUemura, Shuichi Tsutsumi, Akinori Kanai, Yutaka Suzuki, Hiroyuki Aburatani, SumioSugano, Toru Takumi
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] 転写因子BMAL1のゲノム網羅的研究 (2011)
  • Author(s): 畠中史幸、松原千明、Jihwan Myung、寄高崇志、上村直子、堤修一、金井昭教、鈴木譲、油谷浩幸、菅野純夫、内匠透
  • Organizer: 第52回日本生化学会大会中国・四国支部例会
  • Place of Presentation: 広島
• [Presentation] Rhythm and the blues (2011)
  • Author(s): Toru Takumi
  • Organizer: Worldsleep 2011
  • Place of Presentation: Kyoto(招待講演)
• [Presentation] The phosphorylation of PERIOD2 via glycogen synthase kinase 3βhas the possibility of the regulator in the depression-like behavior (2011)
  • Author(s): Yamawaki Y, et al
  • Organizer: The 32nd Naito Conference
  • Place of Presentation: Hokuto, Yamanashi
• [Presentation] Genome-wide profiles of the core clock BMAL1 targets by using multiple high-throughput techniques (2011)
  • Author(s): Toru Takumi
  • Organizer: 第88回日本生理学会大会 第116回日本解剖学会総会・全国学術集会 合同大会
  • Place of Presentation: 横浜
• [Presentation] 転写因子BMAL1のゲノム網羅的研究 (2010)
  • Author(s): 畠中史幸、松原千明、寄高崇志、上村直子、堤修一、金井昭教、鈴木譲、油谷浩幸、菅野純夫、内匠透
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Alterations of locomotor activity dynamics in circadian-clock mutant mice. EMBL Workshop on Translating Behaviour (2009)
  • Author(s): T. Nakamura, T. Takumi, A. Takano, H. Kikuchi, and Y. Yamamoto
  • Organizer: Bridging Clinical and Animal Model Research. EMBL, Heidelberg
  • Place of Presentation: Germany
• [Presentation] 時計と気分 (2009)
  • Author(s): 内匠透
  • Organizer: 第62回日本自律神経学会総会
  • Place of Presentation: 和歌山
• [Presentation] Molecular correlations between circadian rhythm and mood disorder (2009)
  • Author(s): Toru Takumi
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] うつ病ラットモデルを用いたリチウムシグナルとサーカディアンリズム解析 (2009)
  • Author(s): 高野敦子、内匠透
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] Molecular interaction between circadian rhythm and mood disorders (2009)
  • Author(s): Toru Takumi
  • Organizer: XI Congress of the European Biological Rhythms Society
  • Place of Presentation: Strasbourg, France
• [Presentation] Circadian biology of SCN (2009)
  • Author(s): Toru Takumi
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: Kyoto
• [Presentation] Circadian biology of SCN (2009)
  • Author(s): Toru Takumi
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: 京都
• [Presentation] うつ病ラットモデルを用いたリチウムシグナルとサーカディアンリズム解析 (2009)
  • Author(s): 高野敦子・内匠透
  • Organizer: 第82回日本生花学会大会
  • Place of Presentation: 神戸
• [Book] リズムと精神疾患.時間生物学(海老原史樹史、吉村崇編) (2012)
  • Author(s): 内匠透
  • Publisher: 化学同人、京都
• [Book] 時間生物学 (2012)
  • Author(s): 内匠透
  • Total Pages: 2
  • Publisher: 化学同人
• [Book] 体内時計のからくり(NPO法人脳の世紀推進会議編.) (2010)
  • Author(s): 内匠透
  • Publisher: クバプロ.東京
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/anatomy2/index.html