Post-translational histnoe modification and transcriptional regulation.

• Project/Area Number: 21390085
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Nagasaki University
• Principal Investigator: ITO Takashi 長崎大学, 大学院・医歯薬学総合研究科, 教授 (90306275)
• Co-Investigator(Kenkyū-buntansha): NAKAGAWA Takeya 長崎大学, 大学院・医歯薬学総合研究科, 助教 (50363502) ; MIZUSAKI Hirofumi 長崎大学, 大学院・医歯薬学総合研究科, 助教 (40467957)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: DNA高次構浩 / アセチル化 / エピジェネティクス / クロマチン / ヌクレオソーム / ヒストン / ユビキチン化 / リン酸化 / 遺伝子転写 / H2A / 脱ユビキチン化 / 転写開始 / 高次構造 / USP21 / クロストーク

Research Abstract

The genome of the eukaryote is packaged into chromatin, which permits dynamic and broad-ranging changes related to many important biological phenomena. Diverse posttranslational modifications of the core histones occur, often on the tail domains and related to transcriptional regulation. In this project we identified that histone H2A ubiquitylation inhibits histone H3K4 methylation resulting in transcriptional repression. Thus we clarified the role of histone modification and chromatin structure, which related to transcriptional regulation.

Research Products

• [Journal Article] Interferon regulatory factor-4 activates IL-2 and IL-4 promoters in cooperation with c-Rel (2011)
  • Author(s): Shindo, H., Yasui, K., Yamamoto, K., Honma, K., Yui, K., Kohno, T., Ma, Y., Chua, K. J., Kubo, Y., Aihara, H., Ito T, Nagayasu T, Matsuyama T, Hayashi H
  • Journal Title: Cytokine Volume: 56 Pages: 564-572
  • NAID: 120006985203
• [Journal Article] Methionine Adenosyltransferase II Serves as a Transcriptional Corepressor of Maf Oncoprotein (2011)
  • Author(s): Katoh Y, Ikura T, Hoshikawa Y, Tashiro S, Ito T, Ohta M, Kera Y, Noda T, Igarashi K
  • Journal Title: Mol Cell Volume: 41 Pages: 554-566
• [Journal Article] Methionine Adenosyltransferase II Serves as a Transcriptional Corepressor of Maf Oncoprotein. (2011)
  • Author(s): Katoh Y, Ikura T, Hoshikawa Y, Tashiro S, Ito T, Ohta M, Kera Y, Noda T, Igarashi K.
  • Journal Title: Mol Cell Volume: 41 Pages: 554-566
  • Peer Reviewed
• [Journal Article] Transcriptional activation of polycomb-repressed genes by ZRF1 (2010)
  • Author(s): Richly H, Rocha-Viegas L, Ribeiro JD, Demajo S, Gundem G, Lopez-Bigas N, Nakagawa T, Rospert S, Ito T, Di Croce L
  • Journal Title: Nature Volume: 468 Pages: 1124-1128
• [Journal Article] The meiotic recombination checkpoint suppresses NHK-1 kinase to prevent reorganisation of the oocyte nucleus in Drosophila (2010)
  • Author(s): Lancaster OM, Breuer M, Cullen CF, Ito T, Ohkura H
  • Journal Title: PLoS Genet Volume: 6
• [Journal Article] Core histone H2A ubiquitylation and transcriptional regulation (2010)
  • Author(s): Higashi M, Inoue S, Ito T
  • Journal Title: Exp Cell Res Volume: 316 Pages: 2707-2712
• [Journal Article] ヒストンH2Aのユビキチン化と遺伝子転写抑制 (2010)
  • Author(s): 伊藤敬
  • Journal Title: 生化学 Volume: Vol 82 Pages: 232-236
  • NAID: 10026300867
• [Journal Article] The meiotic recombination checkpoint suppresses NHK-1 kin ase to prevent reorganisation of the oocyte nucleus in Drosophila. (2010)
  • Author(s): Lancaster OM, Breuer M, Cullen CF, Ito T, Ohkura H.
  • Journal Title: PLoS Genet Volume: 6
  • Peer Reviewed
• [Journal Article] Transcriptional activation of polycomb-repressed genes by ZRF1. (2010)
  • Author(s): Richly H, Rocha-Viegas L, Ribeiro JD, Demajo S, Gundem G, Lopez-Bigas N, Nakagawa T, Rospert S, Ito T, Di Croce L.
  • Journal Title: Nature Volume: 468 Pages: 1124-1128
  • Peer Reviewed
• [Journal Article] A histone chaperone, DEK, transcriptionally coactivates a nuclear receptor. (2010)
  • Author(s): Sawatsubashi, S., Murata, T., Lim, J., Fujiki, R., Ito, S., Suzuki, E., Tanabe, M., Zhao, Y., Kimura, S., Fujiyama, S., Ueda, T., Umetsu, D., Ito, T., Takeyama, K., Kato, S.
  • Journal Title: Genes Dev 24 Pages: 159-170
  • Peer Reviewed
• [Journal Article] ヒストンH2Aのユビキチン化と遺伝子転写抑制 (2010)
  • Author(s): 伊藤敬
  • Journal Title: 生化学 Vol 82 Pages: 232-236
  • NAID: 10026300867
• [Presentation] Histone modification and transcriptional regulation (2011)
  • Author(s): Takashi Ito
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 会場:パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] 2W2pII-1 Histone modification and transcriptional regulation (2011)
  • Author(s): Takashi Ito
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 3W20-1 Histone Modifications and Transcriptional Regulation (2010)
  • Author(s): Takashi Ito
  • Organizer: 33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Histone Modifications and Transcriptional Regulation (2010)
  • Author(s): Takashi Ito
  • Organizer: Biochemistry and Molecular Biology(BMB) 2010
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] 1S11p-1ヒストン翻訳後修飾と遺伝子転写 (2009)
  • Author(s): 伊藤敬
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-21
• [Presentation] ヒストン翻訳後修飾と遺伝子転写 (2009)
  • Author(s): 伊藤敬
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-21
• [Presentation] 2P0315 Structural analysis of nucleosome assembly protein
  • Author(s): Keisuke Hamada1, Masaaki Shiina1, Risa Nakashima1, Makoto Saito1, Takashi Ito2, Kazuhiro Ogata1
  • Place of Presentation: Yokohama City Univ
• [Presentation] Histone Modification and Transcriptional Regulation
  • Author(s): Takashi Ito
  • Place of Presentation: Nagasaki Univ. School of Med.
• [Remarks]
  • URL: http://www.med.nagasaki-u.ac.jp/biochem/
• [Patent(Industrial Property Rights)] (2009)
  • Inventor(s): 伊藤敬
  • Industrial Property Rights Holder: 伊藤敬、大鵬薬品工業株式会社
  • Industrial Property Number: 2003-008415
  • Acquisition Date: 2009-10-23