Molecular Mechanisms to Regulate The Metabolism of Water and Electrolytes

• Project/Area Number: 21390096
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Kochi University
• Principal Investigator: HONKE Koichi 高知大学, 教育研究部・医療学系, 教授 (80190263)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2009: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: 代謝異常学 / 水・電解質代謝 / アンジオテンシン / アンドステロン合成酵素 / カルシニューリン / 遺伝子発現調節 / スルファチド / バソプレシン / アクアポリン / アルドステロン合成酵素 / 腎尿細管機能 / サルファタイド / アルドステロン / シグナル伝達

Research Abstract

Aiming at elucidation of molecular regulatory mechanisms underlying the reabsorption of water and electrolytes on the distal tubules and collecting ducts of the nephron, we demonstrated calcineurin is involved in the signaling pathway dictating the angiotensin II-dependent aldosteron secretion. Further, we found that deficiency of the mascurin gene causes depletion of the antidiuretic hormone(vasopressin). Finally, amounts of urine and water intake were increased when a high salt concentration of water was given to the sulfated glycolipid-deficient mice. However expression levels and membrane localizations of aquaporin and Na^+, K^+-ATPase were not deteriorated.

Research Products

• [Journal Article] A proteomics approach to the cell-surface interactome using the enzyme-mediated activation of radical sources reaction (2012)
  • Author(s): Jiang S, Kotani N, Ohnishi T, Miyagawa-Yamguchi A, Tsuda M, Yamashita R, Ishiura Y, Honke K
  • Journal Title: Proteomics Volume: 12 Issue: 1 Pages: 54-62
  • DOI: 10.1002/pmic.201100551
  • Peer Reviewed
• [Journal Article] The enzyme-mediated activation of radical source reaction : a new approach to identify partners of a given molecule in membrane microdomains (2011)
  • Author(s): Honke K, Kotani N
  • Journal Title: J. Neurochem Volume: 116 Issue: 5 Pages: 690-695
  • DOI: 10.1111/j.1471-4159.2010.07027.x
  • Peer Reviewed
• [Journal Article] Spatiotemporally-regulated interaction betweenβ1 integrin and ErbB4 that is involved in fibronectin-dependent cell migration (2011)
  • Author(s): Yamashita R, Kotani N, Ishiura Y, Higashiyama S, Honke K
  • Journal Title: J. Biochem Volume: 149 Issue: 3 Pages: 347-355
  • DOI: 10.1093/jb/mvq148
  • NAID: 10030584133
  • Peer Reviewed
• [Journal Article] Hollingsworth MA : Overexpression of GalNAc-transferase GalNAc-T3 promotes pancreatic cancer cell growth (2011)
  • Author(s): Taniuchi K, Cerny RL, Tanouchi A, Kohno K, Kotani N, Honke K, Saibara T
  • Journal Title: Oncogene Volume: 30 Issue: 49 Pages: 4843-4854
  • DOI: 10.1038/onc.2011.194
  • Peer Reviewed
• [Journal Article] A new approach to identify partners of a given molecule in membrane domains. (2011)
  • Author(s): Honke K, Kotani N
  • Journal Title: J.Neurochem. Volume: 116 Pages: 690-695
  • Peer Reviewed
• [Journal Article] Calcineurin mediates the angiotensin II-induced aldosterone synthesis in theadrenal glands by upregulation of transcription of the CYP11B2 gene (2010)
  • Author(s): Yamashiro T, Kuge H, Zhang J, Honke K
  • Journal Title: J. Biochem Volume: 148 Issue: 1 Pages: 115-123
  • DOI: 10.1093/jb/mvq037
  • Peer Reviewed
• [Journal Article] Anomalous expression of Thy1(CD90) in B-cell lymphoma cells and proliferation inhibition by anti-Thy1 antibody treatment (2010)
  • Author(s): Ishiura Y, Kotani N, Yamashita R, Yamamoto H, Kozutsumi Y, Honke K
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 396 Pages: 329-334
  • URL: http://dx.doi.org/10.1016/j.bbrc.2010.04.092
  • Peer Reviewed
• [Journal Article] Calcineurin mediates the angiotensin II-induced aldosterone synthesis in theadrenal glands by upregulation of transcription of the CYP11B2 gene (2010)
  • Author(s): Yamashiro T, Kuge H, Zhang J, Honke K
  • Journal Title: J.Biochem. Volume: 148 Pages: 115-123
  • Peer Reviewed
• [Journal Article] Anomalous Expression of Thy1 (CD90) in B-Cell Lymphoma Cells and Proliferation Inhibition by Anti-Thy1 Antibody Treatment. (2010)
  • Author(s): Ishiura Y, Kotani N, et al.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 396 Pages: 329-334
  • Peer Reviewed
• [Journal Article] Calcineurin mediates the angiotensin II-induced aldosterone synthesis in the adrenal glands by upregulation of transcription of the CYP11B2 gene (2010)
  • Author(s): Yamashiro T, Kuge H, Zhang J, Honke K
  • Journal Title: J.Biochem. (印刷中)
  • NAID: 10027877322
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Honke K, Taniguchi N
  • Journal Title: The Sugar Code.Fundamentals of Glycosciences(Wiley-VCH Verlag GmbH & Co.KGaA) Pages: 385-401
• [Presentation] Analysis of molecular clustering on the living cell membrane (2011)
  • Author(s): Honke K, Miyagawa-Yamaguchi A, Kotani N
  • Organizer: Glycobiology Japan-Netherland Joint Seminar 2011
  • Place of Presentation: 名古屋大学
  • Year and Date: 2011-10-11
• [Presentation] Analysis of molecular clustering on the living cell membrane (2011)
  • Author(s): Honke K, Miyagawa-Yamaguchi A, Kotani N
  • Organizer: Glycobiology Japan-Netherland Joint Seminar 2011
  • Place of Presentation: 名古屋大学(招待講演)
  • Year and Date: 2011-10-11
• [Presentation] 硫酸化糖鎖の生理機能 (2011)
  • Author(s): 本家孝一
  • Organizer: 遺伝子・デリバリー研究会第11回夏期セミナー
  • Place of Presentation: 高知三翠園
  • Year and Date: 2011-08-09
• [Presentation] 硫酸化糖鎖の生理機能 (2011)
  • Author(s): 本家孝一
  • Organizer: 遺伝子・デリバリー研究会第11回夏期セミナー
  • Place of Presentation: 高知三翠園(招待講演)
  • Year and Date: 2011-08-09
• [Presentation] Functions of glycoconjugates in the membrane microdomains in development (2010)
  • Author(s): Honke K, Yamashita T
  • Organizer: The 28th Naito Conference on" Glycan expression and regulation[ I]"
  • Place of Presentation: Shonan, Kanagawa, Japan
• [Presentation] Functions of glycoconjugates in the membrane microdomains in development. (2010)
  • Author(s): Honke K, Yamashita T
  • Organizer: The 28th Naito Conference on "Glycan expression and regulation [I]"
  • Place of Presentation: Shonan, Kanagawa, Japan
• [Book] 脂質 (2011)
  • Author(s): 本家孝一
  • Publisher: メジカルビュー社
• [Book] カラーイラストで学ぶ集中講義生化学 (2011)
  • Author(s): 鈴木敬一郎、本家孝一、大河原知水、藤原範子
  • Publisher: メジカルビュー社
• [Book] Animal models to delineate glycan functionality (2009)
  • Author(s): Honke K, Taniguchi N
  • Publisher: Wiley-VCH Verlag GmbH & Co. KGaA