Expression and functional analyses of G protein-coupled receptor GPR49/LGR5 in human tissues and diseases
| Project/Area Number |
21390108
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Human pathology
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
FUKUMA Mariko 慶應義塾大学, 医学部, 講師(非常勤) (60101995)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
|
|---|
| Keywords | GPR49 / LGR5 / 細胞増殖 / 幹細胞 / 肝癌 / 大腸癌 / 浸潤 / 転移 / GPR49/L / 細胞運動 / 結節性 / GPR49/LGR5 / 腺腫 / G蛋白共役型受容体 / リンパ節転移 / 組織幹細胞 |
|---|
| Research Abstract |
GPR49/LGR5 is reportedly expressed in stem cells of the intestinal crypts and hair follicles of mice, and is overexpressed in some types of cancer. We showed that there was significant overexpression of LGR5 in 35 of 50 colorectal cancers, and in all sporadic colonic adenomas, compared with matched normal mucosa. LGR5 expression correlated significantly with lymphatic invasion, vascular invasion, tumor depth, lymph node metastasis, and tumor stage(IIIC vs. IIIB). In addition to cancer cells, crypt base columnar cells of the small intestine and colon were shown by in situ hybridization to express LGR5. This is the first report suggesting the involvement of LGR5, not only in early events but also in late events in colorectal tumorigenesis. We also overexpressed or down-regulated LGR5 expression in HCC cell lines to explore the function of LGR5 in cancer cells. In vivo and in vitro data suggested that aberrant expression of LGR5 regulates epithelial cell phenotype and survival
|
Report
(4 results)
Research Products
(9 results)
-
[Journal Article] Identification by Differential Tissue Proteome Analysis of Talin-1 as a Novel Molecular Marker of Progression of Hepatocellular Carcinoma2011
Author(s)
Kanamori H, Kawakami T, Effendi K, Yamazaki K, Mori T, Ebinuma H, Masugi Y, Du W, Nagasaka K, Ogiwara A, Kyono Y, Tanabe M, Saito H, Hibi T, Sakamoto M
-
Journal Title
Oncology
Volume: 80
Pages: 406-415
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
