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Expression and functional analyses of G protein-coupled receptor GPR49/LGR5 in human tissues and diseases

Research Project

Project/Area Number 21390108
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionKeio University

Principal Investigator

SAKAMOTO Michiie  慶應義塾大学, 医学部, 教授 (40221270)

Co-Investigator(Renkei-kenkyūsha) FUKUMA Mariko  慶應義塾大学, 医学部, 講師(非常勤) (60101995)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
KeywordsGPR49 / LGR5 / 細胞増殖 / 幹細胞 / 肝癌 / 大腸癌 / 浸潤 / 転移 / GPR49/L / 細胞運動 / 結節性 / GPR49/LGR5 / 腺腫 / G蛋白共役型受容体 / リンパ節転移 / 組織幹細胞
Research Abstract

GPR49/LGR5 is reportedly expressed in stem cells of the intestinal crypts and hair follicles of mice, and is overexpressed in some types of cancer. We showed that there was significant overexpression of LGR5 in 35 of 50 colorectal cancers, and in all sporadic colonic adenomas, compared with matched normal mucosa. LGR5 expression correlated significantly with lymphatic invasion, vascular invasion, tumor depth, lymph node metastasis, and tumor stage(IIIC vs. IIIB). In addition to cancer cells, crypt base columnar cells of the small intestine and colon were shown by in situ hybridization to express LGR5. This is the first report suggesting the involvement of LGR5, not only in early events but also in late events in colorectal tumorigenesis. We also overexpressed or down-regulated LGR5 expression in HCC cell lines to explore the function of LGR5 in cancer cells. In vivo and in vitro data suggested that aberrant expression of LGR5 regulates epithelial cell phenotype and survival

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (9 results)

All 2011 2010

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (1 results)

  • [Journal Article] Identification by Differential Tissue Proteome Analysis of Talin-1 as a Novel Molecular Marker of Progression of Hepatocellular Carcinoma2011

    • Author(s)
      Kanamori H, Kawakami T, Effendi K, Yamazaki K, Mori T, Ebinuma H, Masugi Y, Du W, Nagasaka K, Ogiwara A, Kyono Y, Tanabe M, Saito H, Hibi T, Sakamoto M
    • Journal Title

      Oncology

      Volume: 80 Pages: 406-415

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Endothelin-2 is upregulated in basal cell carcinoma under control of Hedgehog signaling pathway2010

    • Author(s)
      Tanese K, Fukuma M, Ishiko A, Sakamoto M
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 391 Pages: 486-491

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of leucine-rich repeat-containing G protein-coupled receptor 5 in colorectal cancer2010

    • Author(s)
      Uchida H, Yamazaki K, Fukuma M, Yamada T, Hayashida T, Hasegawa H, Kitajima M, Kitagawa Y, Sakamoto M
    • Journal Title

      Cancer Sci

      Volume: 101(7) Pages: 1731-7

    • NAID

      10026595102

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular diagnosis of multistage hepatocarcinogenesis2010

    • Author(s)
      Sakamoto M, Effendi K, Masugi Y
    • Journal Title

      Jpn J Clin Oncol

      Volume: 40(9) Pages: 891-6

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular diagnosis of multistage hepatocarcinogenesis.2010

    • Author(s)
      Sakamoto M, et al.
    • Journal Title

      Jpn J Clin Oncol.

      Volume: 40 Pages: 891-896

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of leucine-rich repeat-containing G protein-coupled receptor 5 in colorectal cancer.2010

    • Author(s)
      Uchida H, Sakamoto M, et al
    • Journal Title

      Cancer Sci

      Volume: 101 Pages: 1731-1737

    • NAID

      10026595102

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Endothelin-2 is upregulated in basal cell carcinoma under control of Hedgehog signaling pathway2010

    • Author(s)
      Tanese K, Fukuma M, Ishiko A, Sakamoto M.
    • Journal Title

      Biochem Biophys Res Commun. 391

      Pages: 486-491

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of leucine-rich repeat-containing G protein-coupled receptor 5 in colorectal cancer2010

    • Author(s)
      Uchida K, Yamazaki K, Fukuma M, Yamada T, Hayashida T, Hasegawa H, Kitajima M, Kitagawa Y, Sakamoto M.
    • Journal Title

      Cancer Sci (in press)

    • NAID

      10026595102

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Integtegrative pathologic and molecular approach for HCC diagnosis2011

    • Author(s)
      Michiie Sakamoto
    • Organizer
      APASL 2^<nd> Hepatocellular Carcinoma Conference
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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