Telomere dysfunction and chromosomal instability in uninvolved tissue with carcinoma by original FISH

• Project/Area Number: 21390109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology
• Principal Investigator: TAKUBO Kaiyo 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究部長 (00154956)
• Co-Investigator(Renkei-kenkyūsha): AIDA Junko 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (80425678)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2009: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
• Keywords: テロメア / FISH / 染色体不安定性 / ヒト癌 / FISH法 / 組織切片 / 老化 / 癌化 / Q-FISH法

Research Abstract

1.We demonstrated telomere dysfunction in the uninvolved epithelium with esophageal squamous cell carcinoma and telomere shortening occurred due to excessive alcohol consumption. 2.We first demonstrated excessive telomere shortening of the epidermis in Werner's syndrome patients. 3.We reported the annual reduction-rate of telomere in the human pituitary gland.

Research Products

• [Journal Article] Short telomeres in an oral precancerous lesion : Q-FISH analysis of leukoplakia (2012)
  • Author(s): Aida J, Kobayashi T, Saku T, Yamaguchi M, Shimomura N, Nakamura KI, Ishikawa N, Maruyama S, Cheng J, Poon SS, Sawabe M, aida T, Takubo K.
  • Journal Title: Journal of Oral Pathology & Medicine Volume: 41(5) Issue: 5 Pages: 372-378
  • DOI: 10.1111/j.1600-0714.2011.01120.x
  • Peer Reviewed
• [Journal Article] Hepatocellular Telomere Length in Biliary Atresia Measured by Q-FISH (2012)
  • Author(s): Sanada Y, Aida J, Kawano Y, Nakamura KI, Shimomura N, Ishikawa N, Arai T, Poon SS, Yamada N, Okada N, Wakiya T, Hayashida M, Saito T, Egami S, Hishikawa S, Ihara Y, Urahashi T, Mizuta K, Yasuda Y, Kawarasaki H, Takubo K.
  • Journal Title: World J Surg Volume: 36 Issue: 4 Pages: 908-916
  • DOI: 10.1007/s00268-012-1453-z
  • Peer Reviewed
• [Journal Article] Telomere dynamics in the human pituitary gland : robust preservation throughout adult life to centenarian age (2011)
  • Author(s): Ishikawa N, Nakamura K, Izumiyama N, Aida J, Sawabe M, Arai T, Kishimoto H, Fujiwara M, Ishii A, Takubo K.
  • Journal Title: AGE Volume: 34 Issue: 4 Pages: 795-804
  • DOI: 10.1007/s11357-011-9280-y
  • Peer Reviewed
• [Journal Article] Palisade vessels as a new histologic marker of esophageal origin in ER Specimens from columnar-lined esophagus (2011)
  • Author(s): Aida J, Vieth M, Ell C, May A, Pech O, Hoshihara Y, Kumagai Y, Kawada K, Hishima T, Tateishi Y, Sawabe M, Arai T, Matsuura M, Takubo K
  • Journal Title: Am J Surg Pathol Volume: 36 Issue: 8 Pages: 1140-1145
  • DOI: 10.1097/pas.0b013e3182206c0e
  • Peer Reviewed
• [Journal Article] Accelerated in vivo epidermal telomere loss in Werner syndrome (2011)
  • Author(s): Ishikawa N, Nakamura K, Izumiyama-Shimomura N, Aida J, Ishii A, Goto M, Ishikawa Y, Asaka R, Matsuura M, Hatamochi A, Kuroiwa M, Takubo K
  • Journal Title: Aging Volume: 3 Pages: 417-429
  • Peer Reviewed
• [Journal Article] Clinicopathologic characteristics of basaloid squamous carcinoma of the esophagus (2011)
  • Author(s): Arai T, Aida J, Nakamura K, Ushio Y, Takubo K
  • Journal Title: Esophagus Volume: 8 Issue: 3 Pages: 169-177
  • DOI: 10.1007/s10388-011-0280-x
  • Peer Reviewed
• [Journal Article] Alcoholics show reduced telomere length in the oesophagus (2011)
  • Author(s): Aida J, Yokoyama A, Izumiyama-Shimomura N, Nakamura K, Ishikawa N, Poon SSS, Fujiwara M, Sawabe M, Matsuura M, Arai T, Takubo K
  • Journal Title: J Pathol Volume: 223 Issue: 3 Pages: 410-416
  • DOI: 10.1002/path.2817
  • URL: http://www.thejournalofpathology.com
  • Peer Reviewed
• [Journal Article] Alcohclics show reduced telomere length in the oesophagus (2011)
  • Author(s): Aida Junko, et al
  • Journal Title: J Pathol Volume: 223 Pages: 410-416
  • Peer Reviewed
• [Journal Article] Accelerated in vivo epidermal tolomere loss in Werner syndrome (2011)
  • Author(s): Ishikawa Naoshi, et al
  • Journal Title: Aging Volume: 3 Pages: 417-429
  • Peer Reviewed
• [Journal Article] Chromosomal instability and telomere lengths of each chromosomal arm measured by Q-FISH in human fibroblast strains prior to replicative senescence (2010)
  • Author(s): Takubo K, Aida J, Izumiyama N, Ishikawa N, Fujiwara M. Poon SSS, Kondo H, Kammori M, Matsuura M, Sawabe M, Arai T, Baird DM, Nakamura K
  • Journal Title: Mech Age Dev Volume: 131 Issue: 10 Pages: 614-624
  • DOI: 10.1016/j.mad.2010.08.007
  • Peer Reviewed
• [Journal Article] Q-FISH analysis of telomere and chromosome instability in the oesophagus with and without squamous cell carcinoma in situ (2010)
  • Author(s): Takubo K, Fujita M, Izumiyama N, Nakamura K, Ishikawa N, Poon SS, Fujiwara M, Sawabe M, Matsuura M, Grabsch H, Arai T, Aida J
  • Journal Title: J Pathol Volume: 221 Issue: 2 Pages: 201-209
  • DOI: 10.1002/path.2704
  • URL: http://www.thejournalofpathology.com
  • Peer Reviewed
• [Journal Article] Changesof telomere length with aging (2010)
  • Author(s): Takubo K, Aida J, Izumiyama-Shimomura N, Ishikawa N, Sawabe M, Kurabayashi R, Shiraishi H, Arai T, Nakamura K
  • Journal Title: Geriatr Gerontol Int Volume: 10(s1) Issue: s1 Pages: 197-206
  • DOI: 10.1111/j.1447-0594.2010.00605.x
  • Peer Reviewed
• [Journal Article] Chromosomal instability and telomere lengths of each chromosomal arm measured by Q-FISH in human fibroblast strains prior to replicative senescence. (2010)
  • Author(s): Takubo K, Aida J, Izumiyama N, et al.
  • Journal Title: Moth Age Dev Volume: 131 Pages: 614-624
  • Peer Reviewed
• [Journal Article] Changes of telomere length with aging. (2010)
  • Author(s): Takubo K, Aida J, Izumiyama-Shimomura N, et al.
  • Journal Title: Geriatr Gerontol Int Volume: 10 Pages: 197-206
  • NAID: 10027273944
  • Peer Reviewed
• [Journal Article] Q-FISH analysis of telomere and chromosome instability in the oesophagus with and without squamous cell carcinoma in situ. (2010)
  • Author(s): Takubo K, Fujita M, Izumiyama N, et al.
  • Journal Title: J Pathol Volume: 221 Pages: 201-209
  • Peer Reviewed
• [Journal Article] Telomere lengths in the oral epithelia with and without carcinoma. (2010)
  • Author(s): Aida J, Izumo T, Shimomura N, et al.
  • Journal Title: Eur J Cancer Volume: 46 Pages: 430-438
  • Peer Reviewed
• [Journal Article] Q-FISH analysis of telomere and chromosome instability in the oesophag us with and without squamous cell carcinoma in situ (2010)
  • Author(s): Takubo K., et al(他11名)
  • Journal Title: Journal of Pathology 22 Pages: 201-209
  • Peer Reviewed
• [Journal Article] Telomerelengths in the oral epithelia with and without carcinoma (2010)
  • Author(s): Aida J., et al(他10名中10番目)
  • Journal Title: European Journal of Cancer 46 Pages: 430-438
  • Peer Reviewed
• [Journal Article] Telomere shortening in Barrett's mucosa and esophageal adenocarcinoma and its association with loss of heterozygosity (2009)
  • Author(s): Shiraishi H, et al.(他8名中8番目)
  • Journal Title: Scandinavian Journal of Gastroenterology 44 Pages: 538-544
  • Peer Reviewed
• [Presentation] テロメア短縮とヒトの老化、癌化 (2011)
  • Author(s): 田久保海誉
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-04-28
• [Presentation] テロメア短縮とヒトの老化、癌化 (2011)
  • Author(s): 田久保海誉、相田順子
  • Organizer: 100回日本病理学会総会
  • Place of Presentation: 横浜
• [Presentation] アルコール症患者の食道上皮のテロメアが短縮している (2011)
  • Author(s): 相田順子、田久保海誉
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
• [Presentation] Q-FISH法による膀胱癌の悪性度とテロメア長 (2011)
  • Author(s): 仲村賢一、田久保海誉
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
• [Presentation] 低リポプロテイン(a)血症では担癌率が高い:高齢者専門病院の連続病理解剖例での検討 (2011)
  • Author(s): 沢辺元司、田久保海誉
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
• [Presentation] Q-FISH法と免疫蛍光抗体法を用いた細胞種類別テロメア長の測定法の開発と膵島細胞種別テロメア長解析 (2011)
  • Author(s): 泉山七生貴、田久保海誉
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
• [Presentation] 2009年ノーベル生理学医学賞課題テロメア : 食道における老化と癌化を結びつけるもの (2010)
  • Author(s): 田久保海誉
  • Organizer: 第76回神奈川食道疾患懇話会
  • Place of Presentation: 横浜
  • Year and Date: 2010-12-04
• [Presentation] テロメアテロメラーゼと創薬 (2010)
  • Author(s): 田久保海誉
  • Organizer: 第64回今堀フォーラム
  • Place of Presentation: 東京
  • Year and Date: 2010-04-20
• [Presentation] 正角化型口腔異型上皮における構成細胞のテロメア長と染色体不安定性についてQ-FISH法による解析 (2010)
  • Author(s): 相田順子、田久保海誉
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] Q-FISH法による培養線維芽細胞を用いたテロメア短縮と染色体異常 (2010)
  • Author(s): 仲村賢一、田久保海誉
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] 胆道閉鎖症の肝組織におけるテロメア長の解析 (2010)
  • Author(s): 眞田幸弘、田久保海誉
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] Q-FISH法による培養線維芽細胞を用いたテロメア短縮と染色体異 (2010)
  • Author(s): 仲村賢一、藤原睦憲、下村-泉山七生貴, ほか
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] 正角化型日腔異型上皮における構成細胞のテロメア長と築色体不安定性についてQ-FISH法による解析 (2010)
  • Author(s): 相田順子、小林孝憲、丸山智, ほか
  • Organizer: 第99即本病理学会総会
  • Place of Presentation: 東京
• [Presentation] 2009年ノーベル生理学医学賞研究テーマ テロメア : 老化と癌化を結びつけるもの (2010)
  • Author(s): 田久保海誉
  • Organizer: 第51回日本臨床細胞学会総会(春期大会)
  • Place of Presentation: 横浜
• [Presentation] アルコール症患者の食道におけるテロメアQ-FISHによる検討 (2010)
  • Author(s): 相田順子、横山顕、下村七生貴, ほか
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] 食道癌は過度にテロメアが短縮し染色体不安定性を伴う粘膜から発生する : Q-FSH 法による解析 (2009)
  • Author(s): 相田順子, 他(他6名中6番目)
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-10-01
• [Presentation] 食道癌はテロメアの短縮した上皮に発生する-Q-FISH法による解析- (2009)
  • Author(s): 相田順子, 他(他5名中5番目)
  • Organizer: 第63回日本食道学会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-06-26
• [Remarks]
  • URL: http://www.ttaggg-rtgp.org/
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  • URL: http://www.tmig.or.jp/J_TMIG/j_research/DA41.html