Analysis of phosphoryl at ionsignals in regulation of stem cell system

• Project/Area Number: 21390119
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Osaka University
• Principal Investigator: KIMURA Tohru 大阪大学, 生命機能研究科, 准教授 (50280962)
• Co-Investigator(Renkei-kenkyūsha): NADA Shigeyuki 大阪大学, 微生物病研究所, 准教授 (50291448)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2009: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: 幹細胞 / 多能性 / リン酸化シグナル / 生殖細胞 / 再生医学 / ES細胞 / iPS細胞 / EG細胞 / Aktシグナル / ERKシグナル / 生殖系列 / 幹細胞システム / 胚性幹細胞(ES細胞) / 始原生殖細胞 / 中胚葉誘導 / 胚性幹細胞 / 中胚葉分化 / 分化可塑性 / 初期化

Research Abstract

Stem cell system is involved in pathological aspects of ti ssues, such as tumorigenesis and aging, as well as in regulation of tissue homeostasis. Tn addition, pluripotent stem cells hold great promise in cell transplantation therapy. Tn this study, we have shown that the phosphorylation signals including Akt signal regulate tumorigenesi s of tissues, acquisition of pluripotency in germl ine cells and induction of iPS(induced pluripotent stem) cells. By manipulation of phosphorylation signal s, we also developed in vitro differentiation system of germ cells from pluripotent stem

Research Products

• [Journal Article] Characterization of dsRNA-induced pancreatitis model reveals the regulatory role of Tffrogulatory factor 2(Trf2) in trypsinogen5 gene transcription (2011)
  • Author(s): Hayashi H, Kohno T, Yasui K, Murota H, Kimura T, Duncan GS, Nakashima T, Yamamoto K, Katayama. I, Ma. Y, Chua. KJ, Suematsu T, himokawa T, Akira S, Kubo Mak TW, and Matsuyama. T
  • Journal Title: Proc. Natl. Acad. Sci, USA Volume: 108(46) Pages: 18766-18771
  • Peer Reviewed
• [Journal Article] Induction of pluripotency in primordial germ cells (2011)
  • Author(s): Kimura. T, and Nakano T
  • Journal Title: Histol. Histopatho Volume: 26(5) Pages: 643-650
  • NAID: 40019072189
  • Peer Reviewed
• [Journal Article] Induction of pluripotency in primordial germ cells (2011)
  • Author(s): Kimura, et al
  • Journal Title: Histol.Histopatho. Volume: 26 Pages: 643-650
  • NAID: 40019072189
  • Peer Reviewed
• [Journal Article] Induction of pluripotency in primordial germ cells. (2011)
  • Author(s): Kimura, et al.
  • Journal Title: Histol.Histopatho. Volume: (印刷中)
  • NAID: 40019072189
  • Peer Reviewed
• [Journal Article] MVH in piRNA processing and gene silencing of retrotransposons (2010)
  • Author(s): Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Takamatsu K, Chuma S, Kojima-Kita K, Shiromoto Y, Asada N, Kimura T, Nakatsuji N, Noce T, Sasaki H, and Nakano T
  • Journal Title: Genes Dev Volume: 24(9) Pages: 887-892
  • Peer Reviewed
• [Journal Article] Metastable primordial germ cell-like state induced from mouse embryonic stem cells by Akt activation (2010)
  • Author(s): Yamano N, Kimura. T, Watanabe-Kushima S, Shinohara. T, and Nakano T
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 392(3) Pages: 311-316
  • Peer Reviewed
• [Journal Article] Metastable primordial germ cell-like state induced from mouse embryonic stem cells by Akt activation. (2010)
  • Author(s): Yamano, et al.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 392 Pages: 311-316
  • Peer Reviewed
• [Journal Article] MVH in piRNA processing and gene silencing of retrotransposons. (2010)
  • Author(s): Kuramochi-Miyagawa, et al.
  • Journal Title: Genes Dev Volume: 24 Pages: 887-892
  • Peer Reviewed
• [Journal Article] Metastable primordial germ cell-like state induced from mouse embryonic stem cells by Akt activation. (2010)
  • Author(s): Yamano, et al.
  • Journal Title: Biochem.Biophys.Res.Commun. (印刷中)
  • Peer Reviewed
• [Journal Article] Associations between PIWI Proteins and TDRD1/ MTR-1 are critical for integrated subcellular localization in murine male germ cells (2009)
  • Author(s): Kojima K, Kuramochi-Miyagawa S, Chuma S, Tanaka T, Nakatsuji N, Kimura T, and Nakano T
  • Journal Title: Genes Cells Volume: 14(10) Pages: 1155-1165
  • Peer Reviewed
• [Journal Article] Associations between PIWI Proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. (2009)
  • Author(s): Kojima, et al.
  • Journal Title: Genes Cells 14 Pages: 1155-1165
  • Peer Reviewed
• [Presentation] DNA methylation of retrotransposon genes is important for meiotic synapsis in spermatogenesis (2011)
  • Author(s): 宮川さとみ
  • Organizer: The 34th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-16
• [Presentation] リン酸化シグナルによる生殖細胞と多能性幹細胞の相互転換 (2011)
  • Author(s): 木村透
  • Organizer: 第12回長崎障害者支援再生医療研究会
  • Place of Presentation: 長崎大学
  • Year and Date: 2011-08-02
• [Presentation] リン酸化シグナルによる生殖細胞と多能性幹細胞の相互転換 (2011)
  • Author(s): 木村透
  • Organizer: 第12回長崎障害者支援再生医療研究会
  • Place of Presentation: 長崎大学(長崎県)(招待講演)
  • Year and Date: 2011-08-02
• [Presentation] Induction of primordial germ cell-like cells from mouse embryonic stem cells by manipulation of signaling pathways (2011)
  • Author(s): 木村透
  • Organizer: The 9^<th> Stem Cell Research Symposium
  • Place of Presentation: 泉ガーデンギャラリー(東京都)
  • Year and Date: 2011-05-13
• [Presentation] Induction of primordial germ cell-like cells from mouse embryonic stem cells by manipulation of signaling pathways (2011)
  • Author(s): Kimura T, Saitou M, Shinohara T, and Nakano T
  • Organizer: The 9th Stem Cell Research Symposium
  • Place of Presentation: 東京都泉ガーデンギャラリー
• [Presentation] 多能性幹細胞と始原生殖細胞の相互転換機構 (2011)
  • Author(s): 木村透、李坤鵬、山野範子、仲野徹
  • Organizer: 第10回日本再生医療学会シンポジウム「iPS・ES細胞の万能性と維持機構の解明～PluriPCtencyの正体とは～」
  • Place of Presentation: 京王プラザホテル
• [Presentation] 多能性幹細胞と始原生殖細胞の相互転換機構 (2011)
  • Author(s): 木村透
  • Organizer: 第10回日本再生医療学会
  • Place of Presentation: 京王プラザホテル(招待講演)
• [Presentation] Induction of the primordial germ cell-like states from mouse embryonic stem cells by manipulation of signaling pathways. (2010)
  • Author(s): Kimura T, Yamano N, Li K-P, Sai tou M, Shinohara T, and Nakano T
  • Organizer: 特定領域研究「生殖系列の世代サイクルとエピゲノムネットワーク」による国際シンポジウムInternational symposium on" Epigenome Network, Developmenta. and Reprogramming of Germ Cells"
  • Place of Presentation: 九州大学
• [Presentation] Metastable primordial germ cell-like stata induced from mouse embryonic stem cells by Akt activation (2010)
  • Author(s): Kimura. T, Yamano.N, Shinohara T, and Nakano T
  • Organizer: The 8^<th> Stem Cell Researeh Sympogium
  • Place of Presentation: 淡路夢舞台国際会議場
• [Presentation] Metastable primordial germ cell-like state induced from mouse embryonic stem cells by Akt activation. (2010)
  • Author(s): Kimura T.
  • Organizer: 第8回 幹細胞シンポジウム
  • Place of Presentation: 淡路夢舞台国際会議場
• [Presentation] Induction of the primordial germ cell-like states from mouse embryonic stem cells by manipulation of signaling pathways. (2010)
  • Author(s): Kimura T.
  • Organizer: International symposium on "Epigenome Network, Development and Reprogramming of Germ Cells"
  • Place of Presentation: 九州大学 医学部100年講堂
• [Presentation] PI3K/ Aktシグナルによる生殖細胞分化と幹細胞システムの制御 (2009)
  • Author(s): 木村透
  • Organizer: 国立成育医療センター研究所セミナー
  • Place of Presentation: 国立成育医療センター研究所
  • Year and Date: 2009-09-04
• [Presentation] An Akt-signal dependent metastable pluripotency state showing primordial germ cell-like gene expression (2009)
  • Author(s): Kimura. T, Yamano N, and Nakano T
  • Organizer: Cold Spring Harbor Symposium Stem Cell Biology Meeting
  • Place of Presentation: Cold Spring Harbor Laboratory, USA
• [Presentation] マウス始原生殖細胞とその幹細胞性の制御 (2009)
  • Author(s): 木村透、山野範子、仲野徹
  • Organizer: 第27回日本受精着床学会総会・学術講演会シンポジウム「生殖医療におけるstem cell biologyとその可能性」
  • Place of Presentation: 国立京都国際会館
• [Presentation] マウス始原生殖細胞とその幹細胞性の制御 (2009)
  • Author(s): 木村透
  • Organizer: 日本受精着床学会
  • Place of Presentation: 国立京都国際会館
• [Presentation] An Akt-signal dependent metastable pluripotency stare showing primordial germ cell-like gene expression. (2009)
  • Author(s): 木村透
  • Organizer: Cold Spring Harbor Symposium
  • Place of Presentation: Cold Spring Harbor Lab (NY, USA)
• [Book] Regulatory Networks in Stem Cells (2009)
  • Author(s): Kimura, Nakano
  • Total Pages: 9
  • Publisher: Humana/Springer