Comprehensive analysis of Bordetella type III effectors

• Project/Area Number: 21390133
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Kitasato University
• Principal Investigator: ABE Akio 北里大学, 大学院・感染制御科学府, 教授 (50184205)
• Co-Investigator(Kenkyū-buntansha): NAGAMATSU Kanna 北里大学, 北里生命科学研究所, 助手 (90458745)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: 百日咳 / ボルデテラ属細菌 / 気管支敗血症菌 / III型分泌装置 / エフェクター / 百日咳菌 / iTRAQ / 分泌タンパク質 / 転写調節

Research Abstract

Bordetella species including Bordetella pertussis deliver effectors into host cells via a type III secretion system(T3SS). Effector-host factor interaction alters host physiological conditions, leading to disease process. In this study, we identified and analyzed new effector(designated BspR) in Bordetella bronchiseptica. BspR effector was translocated into the nucleus, and then interacted with hnRNP H. Interestingly, BspR was a global transcriptional regulator for T3SS and other virulence factors in bacterial cytosol.

Research Products

• [Journal Article] The type III secreted protein BspR regulates the virulence genes in Bordetella bronchiseptica (2012)
  • Author(s): Jun Kurushima, Asaomi Kuwae, Akio Abe
  • Journal Title: PLoS ONE Volume: 7 Issue: 6 Pages: e38925-e38925
  • DOI: 10.1371/journal.pone.0038925
  • Peer Reviewed
• [Journal Article] Btc22 chaperone is required for secretion and stability of the type III secreted protein Bsp22 in Bordetella bronchiseptica (2012)
  • Author(s): Jun Kurushima, Asaomi Kuwae, Akio Abe
  • Journal Title: FEMS Microbiology letters Volume: (印刷中) Issue: 2 Pages: 144-151
  • DOI: 10.1111/j.1574-6968.2012.02561.x
  • Peer Reviewed
• [Journal Article] Iron starvation regulates the type III secretion system in Bordetella bronchiseptica (2012)
  • Author(s): Jun Kurushima, Asaomi Kuwae, Akio Abe
  • Journal Title: Microbiology and Immunology Volume: (印刷中) Issue: 6 Pages: 356-362
  • DOI: 10.1111/j.1348-0421.2012.00442.x
  • Peer Reviewed
• [Journal Article] Solid-state NMR spectroscopy reveals anomer specific transport of galactose in the milk yeast Kluyveromyces lactis (2012)
  • Author(s): Toshio Fukasawa, Akio Abe, Atsusi Nakamura, Miyako Horigome, Akira Naito
  • Journal Title: FEMS Yeast Res Volume: 12 Issue: 4 Pages: 415-422
  • DOI: 10.1111/j.1567-1364.2012.00788.x
  • Peer Reviewed
• [Journal Article] 病原細菌のエフェクターの分泌機構と病原性発揮の分子機構 (2011)
  • Author(s): 阿部章夫, 桑江朝臣
  • Journal Title: 感染炎症免疫 Volume: 41 Pages: 2-11
  • URL: http://jglobal.jst.go.jp/public/20090422/201102263796229560
• [Journal Article] Differential expression of type III effector BteA protein due to IS481 Insertion in Bordetella pertussis (2011)
  • Author(s): Hyun-Ja Han, Asaomi Kuwae, Akio Abe, Yoshichika Arakawa, Kazunari Kamachi
  • Journal Title: PLoS ONE Volume: 6 Issue: 3 Pages: e17797-e17797
  • DOI: 10.1371/journal.pone.0017797
• [Journal Article] A small-molecule inhibitor of the bacterial type III secretion system protects against in vivo infection with Citrobacter rodentium (2011)
  • Author(s): Kyota Kimura, Masato Iwatsuki, Takeshi Nagai, Atsuko Matsumoto, Yoko Takahashi, Kazuro Shiomi, Satoshi Omura and Akio Abe
  • Journal Title: The Journal of Antibiotics Volume: 64 Issue: 2 Pages: 197-203
  • DOI: 10.1038/ja.2010.155
  • NAID: 10030638108
• [Journal Article] Differential expression of type III effector BteA protein due to IS481 insertion in Bordetella pertussis (2011)
  • Author(s): Hyun-Ja Han
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Synergic effect of genotype changes in pertussis toxin and pertactin on adaptation to an acellular pertussis vaccine in the murine intranasal challenge model (2010)
  • Author(s): Eiji Komatsu, Fuminori Yamaguchi, Akio Abe, Alison A. Weiss, and Mineo Watanabe
  • Journal Title: Clin Vaccine Immunol Volume: 17 Issue: 5 Pages: 807-12
  • DOI: 10.1128/cvi.00449-09
• [Journal Article] EspJ effector in enterohemorrhagic E. coli translocates into host mitochondria via an atypical mitochondrial targeting signal (2010)
  • Author(s): Jun Kurushima, Takeshi Nagai, Kanna Nagamatsu, Akio Abe
  • Journal Title: Microbiology and Immunology Volume: 54 Pages: 371-379
  • DOI: 10.1111/j.1348-0421.2010.00218.x
  • NAID: 10027847815
• [Journal Article] Synergic effect of genotype changes in pertussis toxin and pertactin on adaptation to an acellular pertussis vaccine in the murine intranasal challenge model (2010)
  • Author(s): Eiji Komatsu
  • Journal Title: Clinical and Vaccine Immunology Volume: 17 Pages: 807-812
  • Peer Reviewed
• [Journal Article] EspJ effector in enterohemorrhagic E.coli translocates into host mitochondria via an atypical mitochondrial targeting signal (2010)
  • Author(s): Jun Kurushima
  • Journal Title: Microbiology and Immunology 5巻 Pages: 371-379
  • Peer Reviewed
• [Journal Article] Bordetella evades the host immune system by inducing IL-10 through a type III effector (2009)
  • Author(s): Kanna Nagamatsu, Asaomi Kuwae, Tadashi Konaka, Shigenori Nagai, Sei Yoshida, Masahiro Eguchi, Mineo Watanabe, Hitomi Mimuro, Shigeo Koyasu, and Akio Abe
  • Journal Title: J. Exp. Med Volume: 206 Issue: 13 Pages: 3073-3088
  • DOI: 10.1084/jem.20090494
• [Journal Article] 感染現象その理解の深化から疾患制御への展望:細菌の病原因子分泌機構の新展開 (2009)
  • Author(s): 阿部章夫
  • Journal Title: 蛋白質核酸酵素 Volume: 54(8) Pages: 969-974
  • URL: http://search.jamas.or.jp/link/ui/2009214401
• [Journal Article] 特集感染症の現代的課題[細菌のエフェクター輸送システムはどれだけわかっているのか] (2009)
  • Author(s): 阿部章夫
  • Journal Title: 生体の科学 Volume: 60(2) Pages: 110-116
  • URL: http://www.bitway.ne.jp/ejournal/ocn/2425100833.html
• [Journal Article] 病原細菌の分泌装置:その機能と病原性発揮のメカニズム (2009)
  • Author(s): 阿部章夫
  • Journal Title: 感染症学雑誌 Volume: 83(2) Pages: 94-100
  • NAID: 10024803426
  • URL: http://journal.kansensho.or.jp/Disp?pdf=0830020094.pdf
• [Journal Article] Bordetella evades the host immune system by inducing IL-10 througha type III effector, BopN (2009)
  • Author(s): Kanna Nagamatsu
  • Journal Title: J.Exp.Med. 206 Pages: 3073-3088
  • Peer Reviewed
• [Journal Article] 感染現象 その理解の深化から疾患制御への展望:細菌の病原因子分泌機構の新展開 (2009)
  • Author(s): 阿部章夫
  • Journal Title: 蛋白質 核酸 酵素 54 Pages: 969-974
• [Presentation] Functional analysis of a stealth effector, BopN, in Bordetella (2011)
  • Author(s): Akio Abe
  • Organizer: IUMS 2011
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-08
• [Presentation] Functional analysis of a stealth effector, BopN, in Bordetella (2011)
  • Author(s): Akio Abe
  • Organizer: IUMS 2011
  • Place of Presentation: Sapporo Convention Center(北海道)(招待講演)
  • Year and Date: 2011-09-08
• [Presentation] ボルデテラ属細菌の自然免疫回避機構 (2010)
  • Author(s): 阿部章夫
  • Organizer: 第33回 日本分子生物学会年会
  • Place of Presentation: 神戸ポートピア(神奈川県)
  • Year and Date: 2010-12-10
• [Presentation] ボルデテラ属細菌の自然免疫回避機構 (2010)
  • Author(s): 阿部章夫
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸
  • Year and Date: 2010-12-09
• [Presentation] Bordetella immune evasion by type III effector (2010)
  • Author(s): Akio Abe
  • Organizer: The 10th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 兵庫県淡路島
  • Year and Date: 2010-09-08
• [Presentation] Bordetella immune evasion by type III effectors (2010)
  • Author(s): Akio Abe
  • Organizer: The 9th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2010-09-08
• [Presentation] Functional analysis of a stealth effector, BopN, in Bordetella (2010)
  • Author(s): Akio Abe, Kanna Nagamatsu
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-29
• [Presentation] Identification and characterization of a novel type III secreted effector in Bordetella (2010)
  • Author(s): Jun Kurushima, Akio Abe
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-29
• [Presentation] Functional analysis of a stealth effector, BopN, in Bordetella (2010)
  • Author(s): Akio Abe
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2010-03-28
• [Presentation] Bacterial secretion systems as potential targets for drug development (2009)
  • Author(s): 阿部章夫
  • Organizer: 日本放線菌学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-09
• [Presentation] Bordetella evades the most host immune system by inducing IL-10 through a type III effector (2009)
  • Author(s): Akio Abe, Kanna Nagamatsu
  • Organizer: The 9th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 兵庫県淡路島
  • Year and Date: 2009-09-10
• [Presentation] ボルデテラ属細菌の定着における宿主応答抑制機構の解析 (2009)
  • Author(s): 阿部章夫
  • Organizer: 第9回 あわじしま感染症・免疫フォーラム
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2009-09-10
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• [Patent(Industrial Property Rights)] ポリペプチド,ポリペプチドの生産方法および大腸粘膜組織の免疫制御組成物 (2011)
  • Inventor(s): 高橋一郎(10名)
  • Industrial Property Rights Holder: 国立大学法人広島大学(4大学)
  • Filing Date: 2011-02-18
• [Patent(Industrial Property Rights)] ポリペプチド, ポリペプチドの生産方法および大腸粘膜組織の免疫制御組成物 (2011)
  • Inventor(s): 高橋一郎, ら(10名)
  • Industrial Property Rights Holder: 高橋一郎, ら(10名)
  • Industrial Property Number: 2011-033867
  • Filing Date: 2011-02-18