Functional analysis of cellular membrane proteins for generation of viral envelope and transmission
Project/Area Number |
21390137
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Virology
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Research Institution | Kyoto University |
Principal Investigator |
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2009: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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Keywords | BST2 / HIV / エンベロープ / テザリン / 宿主因子 / BiFC / アクセサリー蛋白質 / 膜蛋白質 / BST-2 / Vpu / Bst-2 |
Research Abstract |
We performed a series of virological investigation of cellular membrane proteins on generation of HIV-1 envelope and release of HIV-1 particles. From functional examination of human BST2 that inhibits the release of HIV-1 particles from infected cells on release of viral particles, it was suggested that BST2 acts directly to HIV-1 because human BST2 has ability to inhibit HIV-1 from variety species of cells. Next, we found HIV-1 viral protein U(VPU), a specific antagonist of human BST2, that three amino acid(AA) residues(I34, L37, and L41) in the transmembrane(TM) domain of human BST2 are critical for the interaction with VPUand VPU-mediated antagonism of human BST2. Moreover, computer-assisted structural modeling suggests that an alignment of these four AA residues(I34, L37, L41, and T45) on the same helical face in the TM domain is crucial for the VPU-mediated antagonism of human BST2. These results contribute to the molecular understanding of human BST2 specific antagonism by HIV-1 VPU. On the other hand, we are unable to repeat to show contribution of TIP47 on incorporation of HIV-1 ENV in the viral particles.
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Report
(4 results)
Research Products
(55 results)
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[Journal Article] Huwel, a novel cellular interactor of Gag-Pol through integrase binding, negatively influences HIV-1 infectivity2011
Author(s)
Yamamoto SP, Okawa K, Nakano T, Sano K, Ogawa K, Masuda T, Morikawa Y, Koyanagi Y, Suzuki Y
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Journal Title
Microbes and Infection
Volume: 13
Pages: 339-349
Related Report
Peer Reviewed
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[Journal Article] A novel animal model of Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis in humanized mice2011
Author(s)
Sato K, Misawa N, Nie C, Satou Y, Iwakiri D, Matsuoka M, Takahashi R, Kuzushima K, Ito M, Takada K, Koyanagi Y
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Journal Title
Blood
Volume: 117
Issue: 21
Pages: 5663-5673
DOI
Related Report
Peer Reviewed
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[Journal Article] HIV-1 Vif interacts with TP53 to induce G2 cell cycle arrest and positively regulate viral replication2010
Author(s)
Izumi T, Io K, Matsui M, Shirakawa K, Shinohara M, Nagai Y, Kawahara M, Kobayashi M, Kondoh H, Misawa N, Koyanagi Y, Uchiyama T, Takaori-Kondo A
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Journal Title
Proceedings of the National Academy of Sciences, USA
Volume: 107
Pages: 20798-20803
Related Report
Peer Reviewed
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[Journal Article] Small intestine CD4^+ cell reduction and enteropathy in simian/human immunodeficiency virus KS661-infected rhesus macaques in the presence of low viral load2010
Author(s)
Inaba K, Fukazawa Y, Matsuda K, Himeno A, Matsuyama M, Ibuki K, Miura Y, Koyanagi Y, Nakajima A, Blumberg RS, Takahashi H, Hayami M, Igarashi T, Miura T
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Journal Title
Journal of General Virology 91
Pages: 773-781
Related Report
Peer Reviewed
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[Journal Article] MDM2 is a novel E3 ligase for HIV-1 Vif2009
Author(s)
Izumi T, Takaori-Kondo A, Shirakawa K, Higashitsuji H, Itoh K, Io K, Matsui M, Iwai K, Kondoh H, Sato T, Tomonaga M, Ikeda S, Akari H, Koyanagi Y, Fujita J, Uchiyama T
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Journal Title
NAID
Related Report
Peer Reviewed
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[Journal Article] HIV-1 productive infection in CD4+ effecter memory T lymphocytes and CD4+ T lymphocyte depletion in humanized NOD/SCID/IL2Rγnull mice2009
Author(s)
Nie C, Sato K, Misawa N, Kitayama H, Fujino H, Hiramatsu H, Heike T, Nakahata T, Tanaka Y, Ito M, Koyanagi Y
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Journal Title
Virology 394
Pages: 64-72
Related Report
Peer Reviewed
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