| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Research Abstract |
A liver-specific microRNA, miR-122, has been shown to participate in the efficient replication of hepatitis C virus(HCV). In this study, we examined the possibility of establishing a new permissive cell line for HCV propagation by the expression of miR-122. Among the cell lines we examined, Hep3B cells stably expressing miR-122(Hep3B/ miR-122) exhibited a significant enhancement of HCVcc propagation. Furthermore, a line of" cured" cells established by elimination of HCV RNA from the Hep3B/ miR-122 replicon cells exhibited an enhanced expression of miR-122.
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