Analysis of infection mechanisms of hepatitis C virus and establishment of the indicator cell lines for viral infection
| Project/Area Number |
21390138
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | HCV / 複製 / 宿主因子 / マイクロRNA / 感染 / 指示細胞 / リポーター / ヒト不死化肝細胞 |
|---|
| Research Abstract |
A liver-specific microRNA, miR-122, has been shown to participate in the efficient replication of hepatitis C virus(HCV). In this study, we examined the possibility of establishing a new permissive cell line for HCV propagation by the expression of miR-122. Among the cell lines we examined, Hep3B cells stably expressing miR-122(Hep3B/ miR-122) exhibited a significant enhancement of HCVcc propagation. Furthermore, a line of" cured" cells established by elimination of HCV RNA from the Hep3B/ miR-122 replicon cells exhibited an enhanced expression of miR-122.
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Report
(4 results)
Research Products
(107 results)
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[Journal Article] Elimination of hepatitis C virus from hepatocytes by a selective activation of therapeutic molecules2011
Author(s)
Wen X., Abe T., Kukihara H., Taguwa S., Mori Y., Tani H., Kato N., Suzuki T., Tatsumi M., Moriishi M., and Matsuura Y.
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Journal Title
PLoS One
Volume: 6
Issue: 1
Pages: e15967-e15967
DOI
Related Report
Peer Reviewed
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[Journal Article] Biological and immunological characteristics of hepatitis E virus-like particles based on the crystal structure2009
Author(s)
Yamashita T., Mori Y., Miyazaki N., Cheng H. R., Yoshimura M., Unno H., Shima R., Moriishi K., Tsukihara T., Li T. C., Takeda N., Miyamura T., and Matsuura Y.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 106
Issue: 31
Pages: 12986-12991
DOI
Related Report
Peer Reviewed
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[Journal Article] Human VAP-C negatively regulates hepatitis C virus propagation2009
Author(s)
Kukihara H., Moriishi K., Taguwa S., Tani H., Abe T., Mori Y., Suzuki T., Fukuhara T., Taketomi A., Maehara Y., and Matsuura Y.
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Journal Title
J. Virol
Volume: 83
Issue: 16
Pages: 7959-7969
DOI
Related Report
Peer Reviewed
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[Presentation] Structure-based analysis of hepatitis E virus-like particle. The American Society for Virology2009
Author(s)
Yoshio Mori, Tetsuo Yamashita, Naoyuki Miyazaki, Masato Yoshimura, Hideaki Unno, Kohji Moriishi, Tian-Cheng Li, Naokazu Takeda, R. Holland Cheng, Tomitake Tsukihara, and Yoshiharu Matsuura
Organizer
28th Annual Meeting, The University of British Columbia
Place of Presentation
Vancouver
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