| Project/Area Number |
21390144
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
SAKAI Koji (2010-2011) 国立感染症研究所, エイズ研究センター, 主任研究官 (60260270)
山本 直樹 (2009) National Institute of Infectious Diseases, エイズ研究センター, センター長 (00094053)
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| Co-Investigator(Kenkyū-buntansha) |
RYO Akihide 横浜市立大学, 医学部, 教授 (20363814)
TAKEDA Satoshi 国立感染症研究所, エイズ研究センター, 研究員 (50396959)
阪井 弘治 国立感染症研究所, エイズ研究センター, 主任研究官 (60260270)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Keywords | エイズ / HIV / 移植・再生医療 / ウイルス / 感染症 / AIDS |
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| Research Abstract |
We carried out this study in order to obtain basic information on the prevention of virus infection by novel therapies and on the development of therapeutic drugs against human retroviral infectious diseases utilizing human and macaque iPS cells. We introduced the HIV-1 gp160 membrane glycoprotein gene into human iPS cells, and obtained high expression of the gene. Remarkable cellular immunity was induced in HIV-1 gp160-iPS cells-inoculated mice. Thus we showed the feasibility of human iPS cells as a vector with superior performance for novel AIDS vaccines. We introduced into human iPS cells modified cellular factors engineered to inhibit the propagation of HIV, and succeeded to establish cell lines constitutively expressing either of the genes. We established iPS cells from rhesus monkey dermal fibroblasts, and succeeded to differentiate them to stem cells of blood lineage.
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