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Application of iPS cells to the investigation of the pathogenesis of human retroviral infectious diseases and to the establishment of their prevention and therapy

Research Project

Project/Area Number 21390144
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

SAKAI Koji (2010-2011)  国立感染症研究所, エイズ研究センター, 主任研究官 (60260270)

山本 直樹 (2009)  National Institute of Infectious Diseases, エイズ研究センター, センター長 (00094053)

Co-Investigator(Kenkyū-buntansha) RYO Akihide  横浜市立大学, 医学部, 教授 (20363814)
TAKEDA Satoshi  国立感染症研究所, エイズ研究センター, 研究員 (50396959)
阪井 弘治  国立感染症研究所, エイズ研究センター, 主任研究官 (60260270)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywordsエイズ / HIV / 移植・再生医療 / ウイルス / 感染症 / AIDS
Research Abstract

We carried out this study in order to obtain basic information on the prevention of virus infection by novel therapies and on the development of therapeutic drugs against human retroviral infectious diseases utilizing human and macaque iPS cells. We introduced the HIV-1 gp160 membrane glycoprotein gene into human iPS cells, and obtained high expression of the gene. Remarkable cellular immunity was induced in HIV-1 gp160-iPS cells-inoculated mice. Thus we showed the feasibility of human iPS cells as a vector with superior performance for novel AIDS vaccines. We introduced into human iPS cells modified cellular factors engineered to inhibit the propagation of HIV, and succeeded to establish cell lines constitutively expressing either of the genes. We established iPS cells from rhesus monkey dermal fibroblasts, and succeeded to differentiate them to stem cells of blood lineage.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (6 results)

All 2012 2011

All Journal Article (6 results) (of which Peer Reviewed: 6 results)

  • [Journal Article] Effects of DNA Binding of Zinc Finger and Linkers for Domain Fusion on Catalytic Activity of Sequence-Specific Chimeric Recombinases Determined by a Facile Fluorescent System2012

    • Author(s)
      Nomura, W., Masuda, A., Ohba, K., Urabe, A., Ito, N., Ryo, A., Yamamoto, N., Tamamura, H.
    • Journal Title

      Biochemistry

      Volume: 51 Issue: 7 Pages: 1510-1517

    • DOI

      10.1021/bi201878x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular epidemiological study of human rhinovirus species A, B and C from patients with acute respiratory illnesses in Japan2012

    • Author(s)
      Mika Arakawa, Reiko Okamoto-Nakagawa, Shoichi Toda, Hiroyuki Tsukagoshi, Miho Kobayashi, Akihide Ryo, Katsumi Mizuta, Shunji Hasegawa, Reiji Hirano, Hiroyuki Wakiguchi, Keiko Kudo, Ryota Tanaka, Yukio Morita, Masahiro Noda, Kunihisa Kozawa, Takashi Ichiyama, Komei Shirabe, Hirokazu Kimura
    • Journal Title

      Journal of Medical Microbiology

      Volume: Vol.61 Issue: 3 Pages: 410-419

    • DOI

      10.1099/jmm.0.035006-0

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of phosphorylated proteins involved in the oncogenesis of prostate cancer via Pin1-proteomic analysis2012

    • Author(s)
      Kanji Endoh, Mayuko Nishi, Hitoshi Ishiguro, Hiroji Uemura, Yohei Miyagi, Ichiro Aoki, Hisashi Hirano, Yoshinobu Kubota, Akihide Ryo
    • Journal Title

      Prostate

      Volume: Vol.72 Issue: 6 Pages: 626-637

    • DOI

      10.1002/pros.21466

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular epidemiology of attachment glycoprotein(G) gene in respiratory syncytial virus in children with acute respiratory infection in Japan in 2009/20102012

    • Author(s)
      Ayako Yoshida1, Naoko Kiyota, Miho Kobayashi, Koichi Nishimura, Rika Tsutsui, Hiroyuki Tsukagoshi, Eiko Hirano, Norio Yamamoto, Akihide Ryo, Mika Saitoh, Seiya Harada, Osamu Inoue, Kunihisa Kozawa, Ryota Tanaka, Masahiro Noda, Nobuhiko Okabe, Masato Tashiro, Katsumi Mizuta, Hirokazu Kimura
    • Journal Title

      Journal of Medical Microbiology

      Volume: Vol.6 Issue: 6 Pages: 820-829

    • DOI

      10.1099/jmm.0.041137-0

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vaccination with human induced pluripotent stem cells creates an antigen-specific immune response against HIV-1 gp1602011

    • Author(s)
      Shinji Yoshizaki, Mayuko Nishi, Asami Kondo, Yoshitsugu Kojima, Naoki Yamamoto, Akihide Ryo
    • Journal Title

      Frontiers in Microbiology

      Volume: Vol.2 Pages: 1-8

    • DOI

      10.3389/fmicb.2011.00027

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vaccination with human induced pluripotent stem cells creates an antigen-specific immune response against HIV-1 gp1602011

    • Author(s)
      Shinji Yoshizaki, Mayuko Nishi, Asami Kondo, Yoshitsugu Kojima, Naoki Yamamoto, Akihide Ryo
    • Journal Title

      Frontiers in Virology

      Volume: 2(online journal)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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