Application of iPS cells to the investigation of the pathogenesis of human retroviral infectious diseases and to the establishment of their prevention and therapy

• Project/Area Number: 21390144
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: SAKAI Koji (2010-2011) 国立感染症研究所, エイズ研究センター, 主任研究官 (60260270); 山本 直樹 (2009) National Institute of Infectious Diseases, エイズ研究センター, センター長 (00094053)
• Co-Investigator(Kenkyū-buntansha): RYO Akihide 横浜市立大学, 医学部, 教授 (20363814) ; TAKEDA Satoshi 国立感染症研究所, エイズ研究センター, 研究員 (50396959) ; 阪井 弘治 国立感染症研究所, エイズ研究センター, 主任研究官 (60260270)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: エイズ / HIV / 移植・再生医療 / ウイルス / 感染症 / AIDS

Research Abstract

We carried out this study in order to obtain basic information on the prevention of virus infection by novel therapies and on the development of therapeutic drugs against human retroviral infectious diseases utilizing human and macaque iPS cells. We introduced the HIV-1 gp160 membrane glycoprotein gene into human iPS cells, and obtained high expression of the gene. Remarkable cellular immunity was induced in HIV-1 gp160-iPS cells-inoculated mice. Thus we showed the feasibility of human iPS cells as a vector with superior performance for novel AIDS vaccines. We introduced into human iPS cells modified cellular factors engineered to inhibit the propagation of HIV, and succeeded to establish cell lines constitutively expressing either of the genes. We established iPS cells from rhesus monkey dermal fibroblasts, and succeeded to differentiate them to stem cells of blood lineage.

Research Products

• [Journal Article] Effects of DNA Binding of Zinc Finger and Linkers for Domain Fusion on Catalytic Activity of Sequence-Specific Chimeric Recombinases Determined by a Facile Fluorescent System (2012)
  • Author(s): Nomura, W., Masuda, A., Ohba, K., Urabe, A., Ito, N., Ryo, A., Yamamoto, N., Tamamura, H.
  • Journal Title: Biochemistry Volume: 51 Pages: 1510-1517
  • DOI: 10.1021/bi201878x
  • Peer Reviewed
• [Journal Article] Molecular epidemiological study of human rhinovirus species A, B and C from patients with acute respiratory illnesses in Japan (2012)
  • Author(s): Mika Arakawa, Reiko Okamoto-Nakagawa, Shoichi Toda, Hiroyuki Tsukagoshi, Miho Kobayashi, Akihide Ryo, Katsumi Mizuta, Shunji Hasegawa, Reiji Hirano, Hiroyuki Wakiguchi, Keiko Kudo, Ryota Tanaka, Yukio Morita, Masahiro Noda, Kunihisa Kozawa, Takashi Ichiyama, Komei Shirabe, Hirokazu Kimura
  • Journal Title: Journal of Medical Microbiology Volume: Vol.61 Pages: 410-419
  • DOI: 10.1099/jmm.0.035006-0
  • Peer Reviewed
• [Journal Article] Identification of phosphorylated proteins involved in the oncogenesis of prostate cancer via Pin1-proteomic analysis (2012)
  • Author(s): Kanji Endoh, Mayuko Nishi, Hitoshi Ishiguro, Hiroji Uemura, Yohei Miyagi, Ichiro Aoki, Hisashi Hirano, Yoshinobu Kubota, Akihide Ryo
  • Journal Title: Prostate. Volume: Vol.72 Pages: 626-637
  • DOI: 10.1002/pros.21466
  • Peer Reviewed
• [Journal Article] Molecular epidemiology of attachment glycoprotein(G) gene in respiratory syncytial virus in children with acute respiratory infection in Japan in 2009/2010 (2012)
  • Author(s): Ayako Yoshida1, Naoko Kiyota, Miho Kobayashi, Koichi Nishimura, Rika Tsutsui, Hiroyuki Tsukagoshi, Eiko Hirano, Norio Yamamoto, Akihide Ryo, Mika Saitoh, Seiya Harada, Osamu Inoue, Kunihisa Kozawa, Ryota Tanaka, Masahiro Noda, Nobuhiko Okabe, Masato Tashiro, Katsumi Mizuta, Hirokazu Kimura
  • Journal Title: J Med Microbiol Volume: Vol.6 Pages: 820-829
  • DOI: 10.1099/jmm.0.041137-0
  • Peer Reviewed
• [Journal Article] Vaccination with human induced pluripotent stem cells creates an antigen-specific immune response against HIV-1 gp160 (2011)
  • Author(s): Shinji Yoshizaki, Mayuko Nishi, Asami Kondo, Yoshitsugu Kojima, Naoki Yamamoto, Akihide Ryo
  • Journal Title: Frontiers in Microbiology Volume: Vol.2 Pages: 1-8
  • DOI: 10.3389/fmicb.2011.00027
  • Peer Reviewed
• [Journal Article] Vaccination with human induced pluripotent stem cells creates an antigen-specific immune response against HIV-1 gp160 (2011)
  • Author(s): Shinji Yoshizaki, Mayuko Nishi, Asami Kondo, Yoshitsugu Kojima, Naoki Yamamoto, Akihide Ryo
  • Journal Title: Frontiers in Virology Volume: 2(online journal)
  • Peer Reviewed