Intracellularsignals regulating cytokine production in basophils

• Project/Area Number: 21390149
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Shinshu University
• Principal Investigator: TAKI Shinsuke 信州大学, 医学部, 教授 (50262027)
• Co-Investigator(Renkei-kenkyūsha): HIDA Shigeaki 信州大学, 医学系研究科, 准教授 (10345762) ; SANJO Hideki 信州大学, 医学部, 助教 (50391967)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2009: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: アレルギー / サイトカイン / 好塩基球 / 免疫学 / Th2 / シグナル伝達

Research Abstract

Mechanisms regulating interleukin-4 (IL-4) production in murine basophils were studied. Basophils, when resting,produce IL-4 in response to IL-3 but not F_RI crosslinkage, whereas activated basophils produce the cytokine upon FcεRI crosslinkage but not IL-3 stimulation. The transcriptional repressor Bcl-6 and the suppressive cell surface molecule PIR-B were identified to negatively regulate F_RI signals in resting basophils and IL-3 signals in activated basophils, respectively.

Research Products

• [Journal Article] IL-15 とNK細胞の発生・分化・ホメオスタシス (2013)
  • Author(s): 瀧伸介
  • Journal Title: 臨床免疫・アレルギー科 Volume: 59 Pages: 140-146
  • NAID: 40019567778
• [Journal Article] IRF-2 regulates B cell proliferation and antibody production through distinctmechanisms. (2012)
  • Author(s): Minamoto K, Takahara K, Adachi T, Nagaoka K, Iyoda T, Taki S,Inaba K
  • Journal Title: Int Immunol Volume: 24 Issue: 9 Pages: 573-581
  • DOI: 10.1093/intimm/dxs060
  • NAID: 120005302075
  • Peer Reviewed
• [Journal Article] Differential requirements for IFN regulatory factor-2 in generation of CD1d-independent T cells bearing NK cell receptors (2012)
  • Author(s): Notake T, Horisawa S, Sanjo H, Miyagawa S, Hida S, Taki S.
  • Journal Title: J. Immunol Volume: 188 Issue: 10 Pages: 4838-4845
  • DOI: 10.4049/jimmunol.1200210
  • Peer Reviewed
• [Journal Article] IL-15-high-responder developing NK cells bearing Ly49 receptors in IL-15-/- mice (2011)
  • Author(s): o sh izawa K, Nakajima S, Notake S, Miyagawa S, Hida S, Taki, S
  • Journal Title: J. Immunol Volume: 187 Issue: 10 Pages: 5162-5169
  • DOI: 10.4049/jimmunol.1101561
  • NAID: 120007108237
  • Peer Reviewed
• [Journal Article] Critical role of Th17 cells in inflammation and neovascularization after ischaemia. (2011)
  • Author(s): Hata T, Takahashi M, Hida S, Kawaguchi M, Kashima Y, Usui F, Morimoto H, Nishiyama A, Izawa A, Koyama J, Iwakura Y, Taki S, Ikeda U
  • Journal Title: Cardiovasc Res Volume: 90 Issue: 2 Pages: 362-372
  • DOI: 10.1093/cvr/cvq397
  • Peer Reviewed
• [Journal Article] 活性化T 細胞におけるNKレセプターの発現 (2011)
  • Author(s): 野竹剛、瀧伸介
  • Journal Title: 臨床免疫・アレルギー科 Volume: 55 Pages: 475-482
  • NAID: 40018833143
• [Journal Article] Critical role of Th17 cells in inflammation and neovascularization after ischaemia (2011)
  • Author(s): Hata, T., ほか
  • Journal Title: Cardiovasc Res. Volume: 90 Pages: 362-372
  • Peer Reviewed
• [Journal Article] Potentiation of TLR9 responses for human naive B-cell growth through RP105 signaling (2010)
  • Author(s): Yamazaki K, Yamazaki T, Taki S, Miyake K, Hayashi T, Ochs HD, Agematsu K
  • Journal Title: J. Clin. Immunol. Volume: 135 Issue: 1 Pages: 125-136
  • DOI: 10.1016/j.clim.2009.12.013
  • Peer Reviewed
• [Journal Article] 好塩基球におけるIL-4産生誘導シグナル伝達機構とTh2分化 (2010)
  • Author(s): 小田朗永、瀧伸介
  • Journal Title: 臨床免疫・アレルギー科 Volume: 54 Pages: 546-553
• [Journal Article] Th2 分化と好塩基球 (2010)
  • Author(s): 肥田重明、瀧伸介
  • Journal Title: 実験医学 Volume: 28 Pages: 1879-1884
• [Journal Article] 好塩基球からのIL-4産生誘導機序 (2010)
  • Author(s): 肥田重明、瀧伸介
  • Journal Title: 炎症と免疫 Volume: 18 Pages: 9-13
• [Journal Article] Potentiation of TLR9 responses for human naive B-cell growth through RP105 signaling (2010)
  • Author(s): Yamazaki, K., ほか
  • Journal Title: Clini Immunol. Volume: 135 Pages: 125-136
  • Peer Reviewed
• [Journal Article] Potentiation of TLR9 responses for human naive B-cell growth through RP105 signaling. (2010)
  • Author(s): Yamazaki, K., Yamazaki, T., Taki, S., Miyake, K., Hayashi, T., Ochs, H.D., Agematsu, K.
  • Journal Title: Clini Immunol. 135 Pages: 125-136
  • Peer Reviewed
• [Journal Article] STAT6 signalling is important in CD8+T-cell activation and defence against Toxoplasma gondii infection in the brain. (2009)
  • Author(s): Jin D, Takamoto M, Hu T, Taki S, Sugane K
  • Journal Title: Immunology Volume: 127 Issue: 2 Pages: 187-195
  • DOI: 10.1111/j.1365-2567.2008.02935.x
  • Peer Reviewed
• [Journal Article] Distinct response in maintenance of human naive and memory B cells via IL-21 receptor and TCL1/Akt pathways (2009)
  • Author(s): Nagumo H, Abe J, Kano H, Taki S, Yamazaki K, Yamazaki T, Kobayashi N, Koike K, Sugane K, Saito H, Agematsu K
  • Journal Title: Cell. Immunol. Volume: 256 Issue: 1-2 Pages: 56-63
  • DOI: 10.1016/j.cellimm.2009.01.005
  • Peer Reviewed
• [Journal Article] Fc receptor γ-chain, a constitutive component of the IL-3 receptor, is required for IL-3-induced IL-4 production in basophils. (2009)
  • Author(s): Hida S. Yamasaki S, Sakamoto Y, Takamoto M, Obata K, Takai T, Karasuyama H, Sugane K, Saito T, Taki S
  • Journal Title: Nature Immunol Volume: 10 Issue: 2 Pages: 214-222
  • DOI: 10.1038/ni.1686
  • Peer Reviewed
• [Journal Article] Distinct response in maintenance of human naive and memory B cells via IL-21 receptor and TCL1/Akt pathways. (2009)
  • Author(s): Nagumo H, Abe J, Kano H, Taki S, Yamazaki K, Yamazaki T, Kobayashi N, Koike K, Sugane K, Saito H, Agematsu K.
  • Journal Title: Cell.Immunol. 256 Pages: 56-63
  • Peer Reviewed
• [Journal Article] STAT6 signalling is important in CD8^+ T-cell activation and defence against Toxoplasma gondii infection in the brain. (2009)
  • Author(s): Jin, D., Takamoto, M., Hu, T., Taki, S., Sugane, K.
  • Journal Title: Immunology 127 Pages: 187-195
  • Peer Reviewed
• [Journal Article] 好塩基球のIL-4産生における細胞内シグナル伝達機構 (2009)
  • Author(s): 瀧伸介、肥田重明
  • Journal Title: 実験医学 27 Pages: 3271-3278
• [Journal Article] 好塩基球によるIL-4産生とTh2分化の制御. (2009)
  • Author(s): 瀧伸介
  • Journal Title: アレルギー・免疫 16 Pages: 314-321
• [Presentation] Generation of CD1d-independent T cells bearing various NK receptors requires interferon regulatory factor-2 differentially (2010)
  • Author(s): 野竹剛, ほか
  • Organizer: 14th International Congress of Immuology
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-25
• [Presentation] Paired immunoglobulin-like receptor(PIR)-B attenuates IL-3-induced IL-4 production in a novel ITIM-independent manner in murine basophils (2010)
  • Author(s): 小田朗永, ほか
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-24
• [Presentation] Basophils sense various external signals with their FcR_γ-associated cell surface molecule(s)to produce IL-4 in eliciting Th2 responses (2010)
  • Author(s): 肥田重明, ほか
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-23
• [Presentation] The ITAM-Syk signaling pathway common for divergent IL-4-inducing basophil stimulants (2009)
  • Author(s): 瀧伸介
  • Organizer: 日本免疫学会シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-03
• [Presentation] マウス好塩基球におけるIL-4産生誘導シグナル伝達機構とTh2分化 (2009)
  • Author(s): 瀧伸介
  • Organizer: 日本アレルギー学会終期学術大会
  • Place of Presentation: 秋田
  • Year and Date: 2009-10-28
• [Remarks]
  • URL: http://www.shinshu-u.ac.jp/faculty/medicine/department/doctor/zouki/immunol/main_frame.htm