Research on functional analysis of circulating tumor cells in association with cancer metastasis.

• Project/Area Number: 21390181
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kyoto University
• Principal Investigator: TOI Masakazu 京都大学, 医学研究科, 教授 (10207516)
• Co-Investigator(Kenkyū-buntansha): ISHIGURO Hirosh 京都大学, 医学研究科, 講師 (20422925) ; OHNO Shinji 独立行政法人国立病院機構, 九州がんセンター臨床研究部乳腺科 (50203881) ; UENO Takayuki 京都大学, 医学研究科, 助教 (40452362) ; TSUJI Wakako 京都大学, 医学研究科, 助教 (00531815) ; OKABE Hiroshi 京都大学, 医学研究科, 准教授 (10335250) ; 岩田 広治 愛知がんセンター, 乳腺科, 部長 (90295600)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 腫瘍検査学 / 血中循環癌細胞 / 転移 / アポトーシス / M30 / EpCAM / P-cadherin / 上皮間葉転換 / CTC / EMT / ZEB1 / 乳癌 / サイトケラチン / 混合抗体 / CD44 / cytokeratin / 胃癌

Research Abstract

We investigated the way to improve sensitivity and reproducibility of CTC assay. Recovery rate of basal type phenotype was improved with additional staining with anti-EpCAM antibody and anti-P-cadherin antibody. CTC count and viability in patients with breast cancer who received neoajuvant chemotherapy was also evaluated. Evaluation of CTC viability using M30 may help to evaluate response of neoadjuvant chemotherapy.

Research Products

• [Journal Article] Multi-center study evaluating circulating tumor cells as a surrogate for response to treatment and overall survival in metastatic breast cancer (2010)
  • Author(s): Nakamura S, Yagata H, Ohno S, Yamaguchi H, Iwata H, Tsunoda N, Ito Y, Tokudome N, Toi M, Kuroi K, Suzuki E
  • Journal Title: Breast Cancer Volume: 17(3) Pages: 199-204
  • DOI: 10.1007/s12282-009-0139-3
  • NAID: 10027255780
  • Peer Reviewed
• [Presentation] Evaluation of CTC viability with M30 : Early predictive factor for response of neoadjuvant chemotherapy (2011)
  • Author(s): Noriyoshi Fujisawa, Sunao Tanaka, Takayuki Ueno, Masakazu Toi
  • Organizer: 8th International Symposium on Minimal Residual Cancer
  • Place of Presentation: Osaka
  • Year and Date: 2011-09-21
• [Presentation] Clinical significance of minimal residual cancer in the treatment of breast cancer (2011)
  • Author(s): Masakazu Toi
  • Organizer: 8th International Symposium on Minimal Residual Cancer
  • Place of Presentation: Osaka
  • Year and Date: 2011-09-21
• [Presentation] Clinical significance of minimal residual cancer in the treatment on breast cancer (2011)
  • Author(s): Masakazu Toi
  • Organizer: h International Symposium on Minimal Residual Canc
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2011-09-21
• [Presentation] Evalutaion of CTC viability with M30 : Early predictive factor for response of neoadjuvant chemotherapy (2011)
  • Author(s): Noriyoshi Fujisawa
  • Organizer: h International Symposium on Minimal Residual Canc
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2011-09-21
• [Presentation] 乳癌症例のCTC(循環血液中腫瘍細胞)数の検討 (2010)
  • Author(s): 藤澤憲良, 上野貴之, 竹内恵, 辻和香子, 山城大泰, 石黒洋, 杉江知治, 戸井雅和
  • Organizer: 18回日本乳癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-25
• [Presentation] Circulating tumor cells in primary breast cancer (2010)
  • Author(s): Takayuki Ueno, Tomoharu Sugie, Hiroyasu Yamashiro, Megumi Takeuchi, Wakako Tsuji, Hiroshi Ishiguro, Sunao Tanaka, Masakazu Toi
  • Organizer: Organizaion for Oncology and Translational Research
  • Place of Presentation: Kyoto
• [Presentation] Circulating tumor cells in primary breast cancer (2010)
  • Author(s): Takayuki Ueno
  • Organizer: Organisation for Oncology and Translational Research
  • Place of Presentation: Kyoto
• [Presentation] Circulating tumor cells in primary breast cancer (2009)
  • Author(s): Takayuki Ueno, Sunao Tanaka, Masakazu Toi
  • Organizer: American Society for Cell Biology
  • Place of Presentation: SanDiego
  • Year and Date: 2009-12-06
• [Presentation] Circulating tumor cells in primary breast cancer (2009)
  • Author(s): Takayuki Ueno
  • Organizer: American Society for Cell Biology
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-12-06