Detection and measurement of transthyretin with oxidative modification as a biomarker for disease prevention

• Project/Area Number: 21390184
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: YOSHIKAWA Toshikazu 京都府立医科大学, 医学(系)研究科, 学長 (60128725)
• Co-Investigator(Kenkyū-buntansha): NAITO Yuji 京都府立医科大学, 医学(系)研究科, 准教授 (00305575)
• Co-Investigator(Renkei-kenkyūsha): OSAWA Toshihiko 名古屋大学, 生命農学科, 教授 (00115536) ; AKAIKE Takaaki 熊本大学, 医学(系)研究科, 教授 (20231798)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: トランスサイレチン / メタボリックシンドローム / 血清蛋白質プロファイル / バイオマーカー / 糖尿病 / 疾患バイオマーカー / ELISA法 / プロテインチップ

Research Abstract

Clinical significance of serum transthyretin with oxidative modification was investigated in order to use it as a molecular biomarker for the disease prevention study.

Research Products

• [Journal Article] Regular exercise prevents high-sucrose diet-induced fatty liver via improvement of hepatic lipid metabolism (2011)
  • Author(s): Aoi W, Naito Y, Hang LP, Uchiyama K, Akagiri S, Mizushima K, Yoshikawa T
  • Journal Title: Biochem Biophys Res Commun Volume: 413 Issue: 2 Pages: 330-5
  • DOI: 10.1016/j.bbrc.2011.08.097
• [Journal Article] Dietary whey hydrolysate with exercise alters the plasma protein profile: A comprehensive protein analysis (2011)
  • Author(s): Aoi W, Takanami Y, Kawai Y, Morifuji M, Koga J, Kanegae M, Mihara K, Yanohara T, Mukai J, Naito Y, Yoshikawa T
  • Journal Title: Nutrition Volume: 27 Issue: 6 Pages: 687
  • DOI: 10.1016/j.nut.2010.06.004
• [Journal Article] Oxidative stress-induced posttranslational modification of TRPV1 expressed in esophageal epithelial cells (2011)
  • Author(s): Kishimoto E, Naito Y, Handa O, Okada H, Mizushima K, Hirai Y, Nakabe N, Uchiyama K, Ishikawa T, Takagi T, Yagi N, Kokura S, Yoshida N, Yoshikawa T
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 301 Issue: 2 Pages: G230-G238
  • DOI: 10.1152/ajpgi.00436.2009
• [Journal Article] Heat-shock protein 27(Hsp27) as a target of methylglyoxal in gastrointestinal cancer (2011)
  • Author(s): Oya-Ito T, Naito Y, Takagi T, Handa O, Matsui H, Yamada M, Shima K, Yoshikawa T
  • Journal Title: Biochim Biophys Acta Volume: 1812 Issue: 7 Pages: 769-81
  • DOI: 10.1016/j.bbadis.2011.03.017
  • Peer Reviewed
• [Journal Article] Identification of dihalogenated proteins in rat intestinal mucosa injured by indomethacin (2011)
  • Author(s): Takagi T, Naito Y, Okada H, Okayama T, Mizushima K, Yamada S, Fukumoto K, Inoue K, Takaoka M, Oya-Ito T, Uchiyama K, Ishikawa T, Handa O, Kokura S, Yagi N, Ichikawa H, Kato Y, Osawa T, Yoshikawa T
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 48 Issue: 2 Pages: 178-182
  • DOI: 10.3164/jcbn.10-93
  • NAID: 130004466635
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Carbon monoxide liberated from carbon monoxide-releasing molecule exerts an anti-inflammatory effect on dextran sulfate sodium-induced colitis in mice (2011)
  • Author(s): Takagi T, Naito Y, Uchiyama K, Suzuki T, Hirata I, Mizushima K, Tsuboi H, Hayashi N, Handa O, Ishikawa T, Yagi N, Kokura S, Ichikawa H, Yoshikawa T
  • Journal Title: Dig Dis Sci Volume: 56 Issue: 6 Pages: 1663-71
  • DOI: 10.1007/s10620-010-1484-y
  • Peer Reviewed
• [Journal Article] FGF19 protects colonic epithelial cells against hydrogen peroxide (2011)
  • Author(s): Uchiyama K, Naito Y, Takagi T, Mizushima K, Hayashi N, Handa O, Ishikawa T, Yagi N, Kokura S, Yoshikawa T
  • Journal Title: Digestion Volume: 83 Issue: 3 Pages: 180-3
  • DOI: 10.1159/000321809
  • Peer Reviewed
• [Journal Article] Identification of treatment efficacy-related host factors in chronic hepatitis C by proteinchip serum analysis (2011)
  • Author(s): Fujita N, Naito Y, Yoshikawa T, et al
  • Journal Title: Mol Med Volume: 17 Issue: 1-2 Pages: 70-78
  • DOI: 10.2119/molmed.2010.00124
  • Peer Reviewed
• [Journal Article] The microRNA miR-696 regulates PGC-1{alpha} in mouse skeletal muscle in response to physical activity (2010)
  • Author(s): Aoi W, Naito Y, Mizushima K, Takanami Y, Kawai Y, Ichikawa H, Yoshikawa T
  • Journal Title: Am J Physiol Endocrinol Metab Volume: 298 Issue: 4 Pages: 799-806
  • DOI: 10.1152/ajpendo.00448.2009
• [Journal Article] Regular exercise reduces colon tumorigenesis associated with suppression of iNOS (2010)
  • Author(s): Aoi W, Naito Y, Takagi T, Kokura S, Mizushima K, Takanami Y, Kawai Y, Tanimura Y, Hung LP, Koyama R, Ichikawa H, Yoshikawa T
  • Journal Title: Biochem Biophys Res Commun Volume: 399 Issue: 1 Pages: 14-9
  • DOI: 10.1016/j.bbrc.2010.07.023
• [Journal Article] Reactive oxygen species-quenching and anti-apoptotic effect of polaprezinc on indomethacin-induced small intestinal epithelial cell injury (2010)
  • Author(s): Omatsu T, Naito Y, Handa O, Mizushima K, Hayashi N, Qin Y, Harusato A, Hirata I, Kishimoto E, Okada H, Uchiyama K, Ishikawa T, Takagi T, Yagi N, Kokura S, Ichikawa H, Yoshikawa T
  • Journal Title: J Gastroenterol Volume: 45 Issue: 7 Pages: 692-702
  • DOI: 10.1007/s00535-010-0213-9
  • NAID: 10026706864
• [Journal Article] Ecabet sodium promotes the healing of trinitrobenzene-sulfonic-acid-induced ulceration by enhanced restitution of intestinal epithelial cells (2010)
  • Author(s): Takagi T, Naito Y, Okuda T, Uchiyama K, Adachi S, Mizushima K, Handa O, Kokura S, Ichikawa H, Yoshikawa T
  • Journal Title: J Gastroenterol Hepatol Volume: 25 Issue: 7 Pages: 1259-65
  • DOI: 10.1111/j.1440-1746.2010.06263.x
• [Journal Article] Carbon monoxide enhance colonic epithelial restitution via FGF15 derived from colonic myofibroblasts (2010)
  • Author(s): Uchiyama K, Naito Y, Takagi T, Mizushima K, Hayashi N, Harusato A, Hirata I, Omatsu T, Handa O, Ishikawa T, Yagi N, Kokura S, Yoshikawa T
  • Journal Title: Biochem Biophys Res Commun Volume: 391 Issue: 1 Pages: 1122-6
  • DOI: 10.1016/j.bbrc.2009.12.035
  • Peer Reviewed
• [Journal Article] Oxidative stress markers. (2010)
  • Author(s): Naito Y, Lee M-C, Kato Y, Nagai R, Yonei Y
  • Journal Title: Anti-Aging Medicine Volume: 7 Pages: 36-44
  • NAID: 130000248905
  • Peer Reviewed
• [Journal Article] 医学と医療の最前線ストレス応答プロテオーム解析と消化管疾患 (2010)
  • Author(s): 吉川敏一
  • Journal Title: 日本内科学会雑誌 Volume: 99 Pages: 2548-2557
  • Peer Reviewed
• [Journal Article] 酸化ストレスプロテオミクスにもとづくIBDの診断 (2010)
  • Author(s): 内藤裕二、高木智久、吉川敏一
  • Journal Title: IBD Research Volume: 4 Pages: 294-301
  • Peer Reviewed
• [Journal Article] Relaxant effect of microtitan via regulation of autonomic nerve activity in mice (2009)
  • Author(s): Aoi W, Takanami Y, Kawai Y, Otsuki T, Kawake T, Naito Y, Yoshikawa T
  • Journal Title: Life Sci Volume: 85 Issue: 9-10 Pages: 408-11
  • DOI: 10.1016/j.lfs.2009.07.007
• [Journal Article] Inhibition of Bach1 ameliorates indomethacin-induced intestinal injury in mice (2009)
  • Author(s): Harusato A, Naito Y, Takagi T, Yamada S, Mizushima K, Hirai Y, Horie R, Inoue K, Fukumoto K, Hirata I, Omatsu T, Kishimoto E, Uchiyama K, Handa O, Ishikawa T, Kokura S, Ichikawa H, Muto A, Igarashi K, Yoshikawa T
  • Journal Title: J Physiol Pharmacol Volume: 60 Suppl 7 Pages: 149-54
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20388958
• [Journal Article] Impaired gastric ulcer healing in diabetic mice : role of methylglyoxal (2009)
  • Author(s): Naito Y, Takagi T, Oya-Ito T, Okada H, Suzuki T, Hirata I, Hirai M, Uchiyama K, Handa O, Uchida K, Yoshikawa T
  • Journal Title: J Physiol Pharmacol Volume: 60 Suppl 7 Pages: 123-30
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20388955
• [Journal Article] Oxidative stress-induced posttranslational modification of proteins as a target of functional food (2009)
  • Author(s): Naito Y, Yoshikawa T
  • Journal Title: Forum Nutr Volume: 61 Pages: 39-54
  • DOI: 10.1159/000212737
  • ISBN: 9783805590976, 9783805590983
• [Journal Article] Involvement of reactive oxygen species in indomethacin-induced apoptosis of small intestinal epithelial cells (2009)
  • Author(s): Omatsu T, Naito Y, Handa O, Hayashi N, Mizushima K, Qin Y, Hirata I, Adachi S, Okayama T, Kishimoto E, Takagi T, Kokura S, Ichikawa H, Yoshikawa T
  • Journal Title: J Gastroenterol Volume: 44 Suppl 19 Issue: S19 Pages: 30-4
  • DOI: 10.1007/s00535-008-2293-3
  • NAID: 10024364274
• [Journal Article] Inhalation of carbon monoxide ameliorates collagen-induced arthritis in mice and regulates the articular expression of IL-1beta and MCP-1 (2009)
  • Author(s): Takagi T, Naito Y, Inoue M, Akagiri S, Mizushima K, Handa O, Kokura S, Ichikawa H, Yoshikawa T
  • Journal Title: Inflammation Volume: 32 Issue: 2 Pages: 83-8
  • DOI: 10.1007/s10753-009-9106-6
• [Journal Article] Lansoprazole, a proton pump inhibitor, mediates anti-inflammatory effect in gastric mucosal cells through the induction of heme oxygenase-1 via activation of NF-E2-related factor 2 and oxidation of kelch-like ECH-associating protein 1 (2009)
  • Author(s): Takagi T, Naito Y, Okada H, Ishii T, Mizushima K, Akagiri S, Adachi S, Handa O, Kokura S, Ichikawa H, Itoh K, Yamamoto M, Matsui H, Yoshikawa T
  • Journal Title: J Pharmacol Exp Ther Volume: 331 Issue: 1 Pages: 255-264
  • DOI: 10.1124/jpet.109.152702
  • Peer Reviewed
• [Journal Article] Oxidative Stress-Induced Posttranslational Modification of Proteins asa Target of Functional Food (2009)
  • Author(s): Naito Y, Yoshikawa T.
  • Journal Title: Forum of Nutrition 61 Pages: 39-54
  • Peer Reviewed
• [Journal Article] プロテオミクスによる生活習慣病リスクマーカーの探索 (2009)
  • Author(s): 内藤裕二、吉川敏一
  • Journal Title: 日本体質医学会雑誌 71 Pages: 14-18
  • NAID: 40016569376
  • Peer Reviewed
• [Journal Article] どのようにして統合医療を評価するのか (2009)
  • Author(s): 吉川敏一、内藤裕二
  • Journal Title: 医工学治療 21 Pages: 207-212
  • Peer Reviewed
• [Presentation] Hsp27, a new molecular target for the prevention of GI cancer (2012)
  • Author(s): Naito Y
  • Organizer: Gachon International Symposium on Gastroenterology 2012
  • Place of Presentation: Incheon, Korea
  • Year and Date: 2012-03-31
• [Presentation] Invited Plenary Lecture. Hsp27, a new molecular target for the prevention of Gl cancer (2012)
  • Author(s): Naito Y
  • Organizer: Gachon International Symposium on Gastroenterology 2012
  • Place of Presentation: Incheon
  • Year and Date: 2012-03-31
• [Presentation] シンポジウム「酸化ストレス研究.新しいバイオマーカーを求めて」消化管粘膜におけるPeroxiredoxin-6の役割 (2012)
  • Author(s): 内藤裕二、高木智久、吉川敏一
  • Organizer: 第82回日本衛生学会学術総会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-25
• [Presentation] 炎症性腸疾患診療の最前線 (2012)
  • Author(s): 内藤裕二
  • Organizer: 日本消化器病学会近畿支部会第38回教育講演会
  • Place of Presentation: 大阪
  • Year and Date: 2012-01-28
• [Presentation] Pleiotropic effects of astaxanthin on metabolic diseases (2011)
  • Author(s): Yoshikawa T
  • Organizer: The 3rd World Congress on Ningen Dock
  • Place of Presentation: Taipei
  • Year and Date: 2011-11-27
• [Presentation] Inivited Lecture : Pleiotropic Effects of Exercise and Food Factors on Life-Style Related Diseases.. 2011.11.21 ; Taipei (2011)
  • Author(s): Naito Y
  • Organizer: 2011 International Conference on Food Factors
  • Place of Presentation: Taipei
  • Year and Date: 2011-11-21
• [Presentation] アンチエイジングの実践 (2011)
  • Author(s): 吉川敏一
  • Organizer: 第13回日本医療マネジメント学会学術総会
  • Place of Presentation: 京都
  • Year and Date: 2011-06-24
• [Presentation] シンポジウム「何をどう食べるかの科学」運動と食品因子による大腸癌予防 (2011)
  • Author(s): 内藤裕二, 青井渉, 吉川敏一
  • Organizer: 第11回日本抗加齢医学会総会
  • Place of Presentation: 京都
  • Year and Date: 2011-05-27
• [Presentation] シンポジウム「消化器疾患と微小循環・酸化ストレス」翻訳後修飾タンパク質の解析で見えてきた酸化ストレス研究の新展開 (2011)
  • Author(s): 内藤裕二, 高木智久, 吉川敏一
  • Organizer: 第97回日本消化器病学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-13
• [Presentation] シンポジウム「酸化ストレス研究.臨床から基礎まで」翻訳後修飾タンパク質の解析で見えてきた酸化ストレス研究の新展開 (2011)
  • Author(s): 内藤裕二, 高木智久, 吉川敏一
  • Organizer: 第81回日本衛生医学会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-27
• [Presentation] 先端科学を駆使した食品機能性研究による医農革命 (2010)
  • Author(s): 吉川敏一
  • Organizer: 第8回日本機能性食品医用学会総会
  • Place of Presentation: 大津
  • Year and Date: 2010-12-11
• [Presentation] メタボ時代における機能水の役割 (2010)
  • Author(s): 吉川敏一
  • Organizer: 日本機能水学会第9回学術大会
  • Place of Presentation: 大津
  • Year and Date: 2010-10-30
• [Presentation] 炎症性腸疾患:診断と治療の最前線 (2010)
  • Author(s): 内藤裕二
  • Organizer: 第24回日本消化器病学会甲信越支部教育講演会
  • Place of Presentation: 甲府
  • Year and Date: 2010-10-23
• [Presentation] パネルディスカッション「大腸がん研究の新たな展開と治療戦略」運動による大腸腫瘍抑制作用はSecreted protein acidic and rich cystein(SPARC)を介する (2010)
  • Author(s): 内藤裕二, 高木智久, 吉川敏一
  • Organizer: 第80回日本消化器内視鏡学会総会(JDDW2010)
  • Place of Presentation: 横浜
  • Year and Date: 2010-10-15
• [Presentation] Invited Lecture. New molecular targets for gastric carcinogenesis determined by proteomics. (2010)
  • Author(s): Naito Y, Ito-Oya T, Okada H, Takagi T, Handa O, Yoshikawa T
  • Organizer: Gachon International Gastric Cancer Symposium 2010
  • Place of Presentation: Incheon, Korea
  • Year and Date: 2010-10-09
• [Presentation] ハイパーサーミアの進歩ー原点から未来への展望ー (2010)
  • Author(s): 吉川敏一
  • Organizer: 日本ハイパーサーミア学会第28回総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-09-09
• [Presentation] サプリメントとアンチエイジング.シンポジウム「美は外から,内から」 (2010)
  • Author(s): 吉川敏一
  • Organizer: 第28回日本美容皮膚科学会・学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-08-07
• [Presentation] シンポジウム「抗酸化食品因子の新規な機能探索とその応用」蛋白質の酸化特異的修飾解析による機能性食品有効性評価標的分子の探索 (2010)
  • Author(s): 内藤裕二, 半田修, 青井渉, 高橋二郎, 吉川敏一
  • Organizer: 第63回日本酸化ストレス学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2010-06-25
• [Presentation] 食品による疾病予防とその評価 (2010)
  • Author(s): 吉川敏一
  • Organizer: 日本食品化学学会第16回総会・学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-06-09
• [Presentation] Free radicals and anti-aging (2010)
  • Author(s): Yoshikawa T
  • Organizer: 8th Anti-Aging Medicine World Congress
  • Place of Presentation: Monaco
  • Year and Date: 2010-04-08
• [Presentation] 特別講演医学からみた食品機能 (2010)
  • Author(s): 吉川敏一
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-26
• [Presentation] 胃癌・大腸癌の一次・二次予防に向けて (2010)
  • Author(s): 内藤裕二
  • Organizer: 第24回日本酸化ストレス学会関東支部会
  • Place of Presentation: つくば
  • Year and Date: 2010-01-09
• [Presentation] Pleiotropic effects of exercise on life-style related diseases (2010)
  • Author(s): Yoshikawa T
  • Organizer: The International Conference on Advances in Free Radical Research, Natural Products, Antioxidants and Radioprotectors in Health & Ningth Annual Meeting of the Society for Free Radical Research-India
  • Place of Presentation: India
• [Presentation] 高血糖と消化管疾患.シンポジウムIV・栄養と酸化ストレス (2010)
  • Author(s): 吉川敏一
  • Organizer: 第13回日本病態栄養学会年次学術集会
  • Place of Presentation: 京都
• [Presentation] Peliotropic effects of exercise in life-style related diseases. (2009)
  • Author(s): Naito Y, Aoi W, Takagi T, Yoshikawa T
  • Organizer: International Conference on Nutrition and Physical Activity in Aging, Obesity, and Cancer (NAPA2009)
  • Place of Presentation: Cheju.Korea
  • Year and Date: 2009-12-14
• [Presentation] 口腔運動・機能のアンチエイジングにおける重要性 (2009)
  • Author(s): 吉川敏一
  • Organizer: 第14回NPO法人日本咬合学会学術大会-福岡-
  • Place of Presentation: 福岡
  • Year and Date: 2009-07-04
• [Presentation] シンポジウム「メタボリック症候群と消化器癌」Hsp27タンパク質のメチルグリオキザールによる翻訳後修飾の解析とその細胞生物学的意義 (2009)
  • Author(s): 内藤裕二, 高木智久, 伊藤(大矢)友子
  • Organizer: 第95回日本消化器病学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-05-08
• [Remarks]
  • URL: http://www.kpu-m.ac.jp/k/sun/
• [Remarks]
  • URL: http://www.kpu-m.ac.jp/k/sun/