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Cancer prevention study on the relationship of cancer stem cells biomarker and 8-nitroguanine in cancer tissues

Research Project

Project/Area Number 21390195
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionSuzuka University of Medical Science

Principal Investigator

KAWANISHI Shosuke  鈴鹿医療科学大学, 薬学部, 教授 (10025637)

Co-Investigator(Kenkyū-buntansha) OIKAWA Shinji  三重大学, 大学院・医学系研究科, 准教授 (10277006)
OHNISHI Shiho  鈴鹿医療科学大学, 薬学部, 助手 (80511914)
MA Ning  鈴鹿医療科学大学, 保健衛生学部, 教授 (30263015)
Co-Investigator(Renkei-kenkyūsha) INOUE Sumiko  鈴鹿医療科学大学, 薬学部, 准教授 (20378657)
TABATA Tsutomu  三重大学, 大学院・医学系研究科, 准教授 (40252358)
KOBAYASHI Yoshinao  三重大学, 大学院・医学系研究科, 講師 (70378298)
HORIKI Noriyuki  三重大学, 医学部・付属病院, 講師
IIJIMA Katsunori  東北大学病院, 消化器内科, 助教 (60375003)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords8-ニトログアニン / 8-oxodG / DNA損傷 / 炎症 / 感染 / 活性窒素種 / バイオマーカー / がん予防 / 発がん機構 / 幹細胞 / 一酸化窒素 / プロテオミクス
Research Abstract

8-Nitroguanine was formed in Oct3/4-positive stem cells in S. haematobium-associated cystitis and cancer tissues. 8-Nitroguanine and stem cell marker glypican-3 was observed in Barrett's esophagus tissues. In conclusion, inflammation by various facters may increase the number of mutant stem cells, in which nitrative DNA damage occurs, leading to tumor development.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (57 results)

All 2012 2011 2010 2009 Other

All Journal Article (31 results) (of which Peer Reviewed: 27 results) Presentation (20 results) Book (6 results)

  • [Journal Article] Proton pump inhibitors suppress iNOS-dependent DNA damage in Barrett's esophagus by increasing Mn-SOD expression2012

    • Author(s)
      Thanan R, Ma N, Iijima K, Abe Y, Koike T, Shimosegawa T, Pinlaor S, Hiraku Y, Oikawa S, Murata M, Kawanishi S
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 421(2) Pages: 280-5

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nitrative DNA damage induced by multi-walled carbon nanotube via endocytosis in human lung epithelial cells2012

    • Author(s)
      Guo F, Ma N, Horibe Y, Kawanishi S, Murata M, Hiraku Y
    • Journal Title

      Toxicol Appl Pharmacol

      Volume: 260(2) Pages: 183-92

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of nitrative and oxidative DNA damage in inflammation-related carcinogenesis2012

    • Author(s)
      Murata M, Thanan R, Ma N, Kawanishi S
    • Journal Title

      J Biomed Biotechnol

      Volume: 2012 Pages: 623019-623019

    • DOI

      10.1155/2012/623019

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nuclear Localization of COX-2 in relation to the Expression of Stemness Markers in Urinary Bladder Cancer2012

    • Author(s)
      Thanan R, Murata M, Ma N, Hammam O, Wishahi M, El Leithy T, Hiraku Y, Oikawa S, Kawanishi S
    • Journal Title

      Mediators Inflamm

      Volume: 2012 Pages: 165879-165879

    • DOI

      10.1155/2012/165879

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proton pump inhibitors suppress iNOS-dependent DNA damage in Barrett's esophagus by increasing Mn-SOD expression2012

    • Author(s)
      Thanan R
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 421(2) Pages: 280-285

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inflammation-induced protein carbonylation contributes to poor prognosis of cholangiocarcinoma2012

    • Author(s)
      Thanan, R.
    • Journal Title

      Free Radic.Biol.Med.

      Volume: (印刷中) Issue: 8 Pages: 1465-1472

    • DOI

      10.1016/j.freeradbiomed.2012.01.018

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitrative DNA damage induced by multi-walled carbon nanotube via endocytosis in human lung epithelial cells2012

    • Author(s)
      Guo F
    • Journal Title

      Toxicol Appl Pharmacol

      Volume: 260(2) Pages: 183-192

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitrative DNA damage and Oct3/4 expression in urinary bladder cancer with Schistosoma haematobium infection2011

    • Author(s)
      Ma N, et al
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 414 Issue: 2 Pages: 44-349

    • DOI

      10.1016/j.bbrc.2011.09.073

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nitrative and oxidative DNA damage caused by K-ras mutation in mice2011

    • Author(s)
      Ohnishi, S.
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 413 Issue: 2 Pages: 236-240

    • DOI

      10.1016/j.bbrc.2011.08.076

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanisms of oxidative DNA damage induced by carcinogenic arylamines2011

    • Author(s)
      Murata M, Kawanishi S
    • Journal Title

      Front Biosci

      Volume: 16 Issue: 1 Pages: 1132-43

    • DOI

      10.2741/3739

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nitrative DNA damage and Oct3/4 expression in urinary bladder cancer with Schistosoma haematobium infection2011

    • Author(s)
      Ma N
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 414(2) Pages: 344-349

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Romidepsin(FK228), a potent histone deacetylase inhibitor, induces apoptosis through the generation of hydrogen peroxide2010

    • Author(s)
      Mizutani H, Hiraku Y, Tada-Oikawa S, Murata M, Ikemura K, Iwamoto T, Kagawa Y, Okuda M, Kawanishi S
    • Journal Title

      Cancer Sci

      Volume: 101(10) Issue: 10 Pages: 2214-9

    • DOI

      10.1111/j.1349-7006.2010.01645.x

    • NAID

      10027832563

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA damage and estrogenic activity induced by the environmental pollutant 2-nitrotoluene and its metabolite2010

    • Author(s)
      Watanabe C, Egami T, Midorikawa K, Hiraku Y, Oikawa S, Kawanishi S, Murata M
    • Journal Title

      Environ Health Prev Med

      Volume: 15(5) Issue: 5 Pages: 319-26

    • DOI

      10.1007/s12199-010-0146-1

    • NAID

      10029519330

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protection effect of taurine on nitrosative stress in the mice brain with chronic exposure to arsenic2010

    • Author(s)
      Ma N, Sasoh M, Kawanishi S, Sugiura H, Piao F
    • Journal Title

      J Biomed Sci

      Volume: 17 Suppl 1 Issue: Suppl 1 Pages: S7-S7

    • DOI

      10.1186/1423-0127-17-s1-s7

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The role of iNOS-mediated DNA damage in infection-and asbestos-induced carcinogenesis2010

    • Author(s)
      Hiraku Y, Kawanishi S, Ichinose T, Murata M
    • Journal Title

      Ann N Y Acad Sci

      Volume: 1203 Issue: 1 Pages: 15-22

    • DOI

      10.1111/j.1749-6632.2010.05602.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of aging brain in SAMP10 mouse : a model of age-related cerebral degeneration.2010

    • Author(s)
      Furukawa A.
    • Journal Title

      Mech.Ageing Dev.

      Volume: 131(6) Pages: 379-388

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Romidepsin (FK228), a potent histone deacetylase inhibitor, induces apoptosis through the generation of hydrogen peroxide.2010

    • Author(s)
      Mizutani H.
    • Journal Title

      Cancer Sci.

      Volume: 101(10) Pages: 2214-2219

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The role of iNOS-mediated DNA damage in infection- and asbestos- induced carcinogenesis.2010

    • Author(s)
      Hiraku Y.
    • Journal Title

      Ann N Y Acad Sci.

      Volume: 1203 Pages: 15-22

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oxidatively generated DNA damage induced by 3-amino-5-mercapto-1,2,4-triazole, a metabolite of carcinogenic amitrole.2010

    • Author(s)
      Furukawa A.
    • Journal Title

      Mutat Res.

      Volume: 694(1-2) Pages: 7-12

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protection effect of taurine on nitrosative stress in the mice brain with chronic exposure to arsenic.2010

    • Author(s)
      Ma N.
    • Journal Title

      J Biomed Sci.

      Volume: 17(Suppl 1):S7 Pages: 1-6

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA damage and estrogenic activity induced by the environmental pollutant 2-nitrotoluene and its metabolite.2010

    • Author(s)
      Watanabe C.
    • Journal Title

      Environ Health Prev Med.

      Volume: 15(5) Pages: 319-326

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2010

    • Author(s)
      平工雄介
    • Journal Title

      第7章 生体有害金属、7.3鉛、食品安全ハンドブック(食品安全ハンドブック編集委員会 編)(丸善株式会社)

      Pages: 122-125

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2010

    • Author(s)
      Kawanishi S.
    • Journal Title

      DNA Adducts : Formation, Detection and Mutagenesis(DNA, Properties and Modifications, Functions and Interactions, Recombination and Applications)(Nova Science Pub Inc)

      Pages: 169-181

    • Related Report
      2009 Annual Research Report
  • [Journal Article] DNA damage and apoptosis induced by photosensitization of 5, 10, 15, 20-tetrakis(N-methyl-4-pyridyl)-21H, 23H-porphyrin via singlet oxygen generation2009

    • Author(s)
      Tada-Oikawa S, Oikawa S, Hirayama J, Hirakawa K, Kawanishi S
    • Journal Title

      Photochem Photobiol

      Volume: 85(6) Issue: 6 Pages: 1391-9

    • DOI

      10.1111/j.1751-1097.2009.00600.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Damage to cellular and isolated DNA induced by a metabolite of aspirin2009

    • Author(s)
      Oikawa S, Kobayashi H, Tada-Oikawa S, Isono Y, Kawanishi S
    • Journal Title

      Mutat Res

      Volume: 661(1-2) Issue: 1-2 Pages: 93-100

    • DOI

      10.1016/j.mrfmmm.2008.11.016

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protective effect of taurine against nitrosative stress in the stomach of rat with water immersion restraint stress2009

    • Author(s)
      Ma N, Sasaki T, Sakata-Haga H, Ohta K, Gao M, Kawanishi S, Fukui Y
    • Journal Title

      Adv Exp Med Biol

      Volume: 643 Pages: 273-83

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical analysis of 8-nitroguanine, a nitrative DNA lesion, in relation to inflammation-associated carcinogenesis2009

    • Author(s)
      Hiraku Y, Kawanishi S
    • Journal Title

      Methods Mol Biol

      Volume: 512 Pages: 3-13

    • DOI

      10.1007/978-1-60327-530-9_1

    • ISBN
      9781603275293, 9781603275309
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA damage and apoptosis induced by photosensitization of 5, 10, 15, 20-tetrakis(N-methyl-4-pyridyl)-21H, 23H-porphyrin via singlet oxygen generation.2009

    • Author(s)
      Tada-Oikawa S
    • Journal Title

      Photochem Photobiol. 85(6)

      Pages: 1391-1399

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protective effect of taurine against nitrosative stress in the stomach of rat with water immersion restraint stress.2009

    • Author(s)
      Ma N.
    • Journal Title

      Adv Exp Med Biol. 643

      Pages: 273-283

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      Hiraku, Y.
    • Journal Title

      Immunohistochemical analysis of 8-nitroguanine, a nitrative DNA lesion, in relation to inflammation-associated carcinogenesis, In : Methods in Molecular Biology. vol.512, Inflammation and Cancer, Methods and Protocols : Volume 2. Molecular Analysis and Pathways(Kozlov, S.V., Editor)(Humana Press, New York, NY, USA)

      Pages: 3-13

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      川西正祐
    • Journal Title

      炎症・再生医学事典(朝倉書店)

      Pages: 328-331

    • Related Report
      2009 Annual Research Report
  • [Presentation] 神経変性疾患における酸化損傷タンパク質の同定2012

    • Author(s)
      及川伸二
    • Organizer
      日本酸化ストレス学会東海支部設立シンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2012-02-18
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] RRAD with tumor suppressive function is aberrantly methylated in nasopharyngeal carcinoma2012

    • Author(s)
      Mo Yingxi
    • Organizer
      第82回日本衛生学会総会
    • Place of Presentation
      京都
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 上咽頭癌におけるTFPI-2プロ及川伸二,虚血再灌流後のサル黒質におけるカルボニル化タンパク質の同定2012

    • Author(s)
      王淑民
    • Organizer
      第82回日本衛生学会総会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] Protein carbonylation in liver fluke-induced cholangiocarcinoma in relation to poor prognosis2012

    • Author(s)
      Thanan Raynoo
    • Organizer
      Mahidol International Conference on Infection and Cancer
    • Place of Presentation
      タイ国バンコク市
    • Related Report
      2011 Annual Research Report
  • [Presentation] 虚血再灌流後のサル黒質におけるカルボニル化タンパク質の同定2012

    • Author(s)
      及川伸二
    • Organizer
      第82回日本衛生学会総会
    • Place of Presentation
      京都
    • Related Report
      2011 Annual Research Report
  • [Presentation] 二酸化チタンナノ粒子による細胞毒性作用の解析2012

    • Author(s)
      及川(多田)佐枝子
    • Organizer
      第82回日本衛生学会総会
    • Place of Presentation
      京都
    • Related Report
      2011 Annual Research Report
  • [Presentation] 上咽頭癌におけるTFPI-2プロモーター領域のメチル化によるがん抑制機能の不活化2012

    • Author(s)
      王淑民
    • Organizer
      第82回日本衛生学会総会
    • Place of Presentation
      京都
    • Related Report
      2011 Annual Research Report
  • [Presentation] 多層カーボンナノチューブで処理したヒト肺上皮細胞におけるニトロ化DNA損傷2011

    • Author(s)
      Guo Feiye
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 寄生虫感染による幹様細胞における酸化・ニトロ化DNA損傷2011

    • Author(s)
      Thanan Raynoo
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] 上咽頭がんにおけるゲノムワイドなメチル化DNAの解析2011

    • Author(s)
      Mo Yingxi
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2011-10-03
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Epigenetic inactivation of paracrined-FSTL1 modulates macrophage functions in nasopharyngeal carcinoma2011

    • Author(s)
      Xiao Xue
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2011-10-03
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 発がん性クメンの光照射による酸化的DNA損傷2011

    • Author(s)
      大西志保
    • Organizer
      第33回日本光医学・光生物学会
    • Place of Presentation
      大阪府吹田市
    • Year and Date
      2011-07-22
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 紫外線誘発皮膚癌のモデルマウスにおける8-ニトログアニン生成2011

    • Author(s)
      馬寧
    • Organizer
      第33回日本光医学・光生物学会
    • Place of Presentation
      大阪府吹田市
    • Year and Date
      2011-07-22
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Genome-wide profiling of DNA methylation identifies epigenetic targets and aberrant methylation of TFPI2 in nasopharyngeal carcinoma2011

    • Author(s)
      Mo Yingxi
    • Organizer
      日本環境変異原学会第40回大会
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Role of mutagenic 8-nitroguanine in estrogen-dependent radiation-induced mammary tumorigenesis of rats2011

    • Author(s)
      S. Kawanishi, N. Ma, M. Onoda, H. Inano, S. Ohnishi, M. Murata
    • Organizer
      14th International Congress of Radiation Research
    • Place of Presentation
      Warsaw, Poland
    • Related Report
      2011 Final Research Report
  • [Presentation] 天然色素プルプリンによるDNA損傷機構の解明2011

    • Author(s)
      及川伸二
    • Organizer
      がん予防大会2011京都(第18回日本がん予防学会)
    • Place of Presentation
      京都
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Role of mutagenic 8-nitroguanine in estrogen-dependent radiation-induced mammary tumorigenesis of rats2011

    • Author(s)
      Kawanishi Shosuke
    • Organizer
      14th International Congress of Radiation Research
    • Place of Presentation
      ポーランド国ワルシャワ市
    • Related Report
      2011 Annual Research Report
  • [Presentation] K-ras activation and oxidative DNA damage2009

    • Author(s)
      S. Kawanishi, S. Ohnishi, H. Saito, N. Suzuki, Y. Hiraku, M. Murata
    • Organizer
      2009 Annual Meeting of the Society for Free Radical Research-Europe
    • Place of Presentation
      Rome, Italy
    • Related Report
      2011 Final Research Report
  • [Presentation] K-ras activation and oxidative DNA damage2009

    • Author(s)
      Kawanishi S.
    • Organizer
      2009 Annual Meeting of the Society for Free Radical Research-Europe
    • Place of Presentation
      イタリア国 ローマ市
    • Related Report
      2009 Annual Research Report
  • [Presentation] The Role of iNOS-Mediated DNA Damage in Inflammation-Related Care inogenesis2009

    • Author(s)
      Hiraku Y.
    • Organizer
      4th International Conference on Oxidative/Nitrosative Stress and Disease
    • Place of Presentation
      アメリカ合衆国 ニューヨーク市
    • Related Report
      2009 Annual Research Report
  • [Book] Potential Biomarker to Evaluate the Risk of Inflammation-Related Carcinogenesis2012

    • Author(s)
      N. Ma, M. Murata, S. Ohnishi, R. Thanan, Y. Hiraku, S. Kawanishi
    • Publisher
      InTech
    • Related Report
      2011 Final Research Report
  • [Book] BIOMARKER2012

    • Author(s)
      Ma Ning
    • Publisher
      InTech
    • Related Report
      2011 Annual Research Report
  • [Book] Oxidative DNA Damage and DNA Adducts Induced by Polycyclic Aromatic Hydrocarbons and Aromatic Amines2010

    • Author(s)
      S. Kawanishi, S. Ohnishi
    • Publisher
      Nova Science Pub Inc
    • Related Report
      2011 Final Research Report
  • [Book] K-ras activation and oxidative DNA damage2009

    • Author(s)
      S. Kawanishi, S. Ohnishi
    • Related Report
      2011 Final Research Report
  • [Book] 炎症・再生医学事典2009

    • Author(s)
      川西正祐、大西志保
    • Publisher
      朝倉書店
    • Related Report
      2011 Final Research Report
  • [Book] Prognostic significance of nitrative DNA damage in infection- and inflammation-related carcinogenesis, In : Nitric Oxide and Cancer : Prognosis, Prevention and Therapy

    • Author(s)
      Hiraku, Y.
    • Publisher
      Springer, New York, NY, USA(印刷中)
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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