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Screening, identification and forensic application of age-related biomolecules

Research Project

Project/Area Number 21390215
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionUniversity of Fukui

Principal Investigator

IIDA REIKO  福井大学, 医学部, 准教授 (40139788)

Co-Investigator(Renkei-kenkyūsha) TAKESHITA Haruo  島根大学, 医学部, 教授 (90292599)
Project Period (FY) 2009-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords法医学 / 遺伝子発現 / 年齢推定 / 老化
Research Abstract

1. To search for age-dependently expressed genes, cells were transfected with siRNA duplexs targeting either Rhit or M-LP. Statistically significant alteration was observed in the expression of sixty four genes. 2. RhitH was confirmed to be involved in transcriptional regulation of M-LPH. 3. Cis-regulatory elements in the RhitH gene were searched, and two transcription factors were identified: FOXD3 as a negative regulatory element, and GABP, one of the key regulators of the mitochondrial electron transport system, as a positive regulatory element. 4. M-LPH functions to protect cells from oxidative stress and/or initiation of the mitochondrial apoptotic cascade under stressed conditions. 5. Simple methods for genotyping of SNPs in the Gpx and RhitH genes were established. 6. The mRNA levels of RhitH and M-LPH in adult and fetal tissues were analyzed. The M-LPH levels in adult tissues were much higher than those in fetal tissues.

Report

(7 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Products Report
  • Research Products

    (29 results)

All 2014 2013 2012 2011 2010 2009

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (17 results)

  • [Journal Article] Three non- synonymous single nucleotide polymorphisms in the RhitH gene cause reduction of the repression activity that leads to up-regulation of M-LPH, a participant in mitochondrial function2014

    • Author(s)
      R. Iida, M. Ueki, J. Fujiwara, H. Takeshita, K. Kimura-Kataoka, T. Yasuda
    • Journal Title

      BioResearch Open Access

      Volume: 2 Pages: 440-447

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Three non-synonymous single nucleotide polymorphisms in the RhitH gene cause reduction of the repression activity that leads to up-regulation of M-LPH, a participant in mitochondrial function2013

    • Author(s)
      R. Iida, M. Ueki, J.Fujiwara, H. Takeshita, K. Kimura-Kataoka, T. Yasuda
    • Journal Title

      BioResearch Open Access

      Volume: 2 Issue: 6 Pages: 440-447

    • DOI

      10.1089/biores.2013.0042

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of RhitH as a novel transcriptional repressor of human Mpv17- like protein with a mitigating effect on mitochondrial dysfunction, and its transcriptional regulation by FOXD3 and GABP2012

    • Author(s)
      R. Iida, M. Ueki, T. Yasuda
    • Journal Title

      Free Rad Biol Med

      Volume: 52 Pages: 1413-1422

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of Rhit as a novel transcriptional repressor of human Mpv17-like protein with a mitigating effect on mitochondrial dysfunction, and its transcriptional regulation by FOX D3 and GABP2012

    • Author(s)
      R.Iida, et al
    • Journal Title

      Free Radical Biology and Medicine

      Volume: 52 Issue: 8 Pages: 1413-1422

    • DOI

      10.1016/j.freeradbiomed.2012.01.003

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nonsynonymous single-nucleotide polymorphisms of the human apoptosis-related endonuclease-DNA fragmentation factor beta polypeptide, endonuclease G, and Flap endonuclease 1-genes show a low degree of genetic heterogeneity2012

    • Author(s)
      H.Takeshita
    • Journal Title

      DNA and Cell Biology

      Volume: 31 Issue: 1 Pages: 36-42

    • DOI

      10.1089/dna.2011.1293

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ヒトSOD1, SOD2 およびSOD3遺伝子内の非同義置換型SNPの同時解析-血清SOD活性と多型性SNP との相関について2011

    • Author(s)
      飯田 礼子, 安田 年博, 植木 美鈴, 竹下 治男
    • Journal Title

      DNA多型

      Volume: 19 Pages: 275-280

    • NAID

      10029669039

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ヒトSOD1、SOD2およびSOD3遺伝子内の非同義置換型SNPの同時解析2011

    • Author(s)
      飯田礼子, 他
    • Journal Title

      DNA多型

      Volume: 19 Pages: 275-280

    • Related Report
      2011 Annual Research Report Products Report
    • Peer Reviewed
  • [Journal Article] A novel transcriptional repressor, Rhit, is involved in heat-inducible and age-dependent expression of Mpv17-like protein, a participant in reactive oxygen species metabolism2010

    • Author(s)
      R. Iida, M. Ueki, T. Yasuda
    • Journal Title

      Mol Cell Biol

      Volume: 30 Pages: 2306-2315

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Transcriptional Repressor,Rhit,Is Involved inHeat-Inducible and Age-Dependent Expression of Mpv17-Like Protein,a Participant in Reactive Oxygen Species Metabolism.2010

    • Author(s)
      R.Iida, et al.
    • Journal Title

      Mol.Cell.Biol.

      Volume: 30 Pages: 2306-2315

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Transcriptional Repressor, Rhit, Is Involved in Heat-Inducible and Age-Dependent Expression of Mpv17-Like Protein, a Participant in Reactive Oxygen Species Metabolism2010

    • Author(s)
      R.Iida, et al.
    • Journal Title

      Mol.Cell.Biol. (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous genotyping of 11 non-synonymous SNPs in the 4 glutathione peroxidase genes using the multiplex single base extension method2009

    • Author(s)
      R. Iida, E. Tsubota, I. Yuasa, H. Takeshita, T. Yasuda
    • Journal Title

      Clin Chim Acta

      Volume: 402 Pages: 79-82

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous genotyping of eleven non-synonymous SNPs in the four glutathione peroxidase genes using the multiplex single base extension method2009

    • Author(s)
      R.Iida, et al.
    • Journal Title

      Clin.Chim.Acta 402

      Pages: 79-82

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 転写因子RhitH 遺伝子における非同義置換型SNPの遺伝的解析と転写抑制に及ぼす効果2013

    • Author(s)
      飯田 礼子, 他
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2013-09-12
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト年齢依存性転写抑制因子RhitH の同定とその転写調節機構2013

    • Author(s)
      安田 年博, 飯田 礼子, 他
    • Organizer
      第97次日本法医学会学術全国集会
    • Place of Presentation
      札幌
    • Year and Date
      2013-06-27
    • Related Report
      2013 Final Research Report
  • [Presentation] 転写因子RhitH遺伝子における非同義置換型SNPの遺伝的解析と転写抑制に及ぼす効果2013

    • Author(s)
      飯田 礼子, 安田 年博, 竹下 治男
    • Organizer
      第86回日本生化学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒト年齢依存性転写抑制因子RhitHの同定とその転写調節機構2013

    • Author(s)
      安田 年博, 飯田 礼子, 竹下 治男, 小湊 慶彦
    • Organizer
      第97次日本法医学会学術全国集会
    • Place of Presentation
      ロイトン札幌(札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of a novel transcriptional repressor, RhitH, involved in expression of human Mpv17-like protein, and its transcriptional regulation by FOXD3 and GABP2012

    • Author(s)
      R. Iida et al
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of a novel transcriptional repressor, RhitH, involved in expression of human Mpv17-like protein, and its transcriptional regulation by FOXD3 and GABP2012

    • Author(s)
      R.Iida
    • Organizer
      日本生化学会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Identification of a novel transcriptional repressor, Rhit, involved in heat-inducible and age-dependent expression of Mpv 17-like protein2011

    • Author(s)
      R. Iida et al
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of a novel transcriptional repressor, Rhit, involved in heat-inducible and age-dependent expression of Mpv17-like protein2011

    • Author(s)
      R.Iida, et al
    • Organizer
      日本生化学会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 年齢依存性を示す新規なマウス転写抑制因子Rhitの同定2011

    • Author(s)
      安田 年博, 飯田 礼子, 他
    • Organizer
      第95次日本法医学会学術全国集会
    • Place of Presentation
      福島
    • Year and Date
      2011-06-17
    • Related Report
      2013 Final Research Report
  • [Presentation] 年齢依存性を示す新規な転写抑制因子Rhitの同定2011

    • Author(s)
      飯田礼子, 他
    • Organizer
      日本法医学会
    • Place of Presentation
      コラッセ福島(福島県)
    • Year and Date
      2011-06-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Multiplex single base extension method for simultaneous genotyping of non-sy nonymous SNP in the four human glutathione peroxidase genes2010

    • Author(s)
      R. Iida et al
    • Organizer
      日本分子生物学会・日本生化学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2013 Final Research Report
  • [Presentation] Multiplex single base extension methodfor simultaneous genotyping of non-sy nonymous SNP in the fbur humang glutathione peroxidase genes2010

    • Author(s)
      飯田礼子, 他
    • Organizer
      日本分子生物学会・日本生化学会合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒトSOD1, SOD2およびSOD3遺伝子内の非同義置換型SNPの同時解析2010

    • Author(s)
      安田 年博, 飯田 礼子, 他
    • Organizer
      日本DNA多型学会第19回学術集会
    • Place of Presentation
      三島
    • Year and Date
      2010-11-18
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒトSOD1、SOD2およびSOD3遺伝子内の非同義置換型SNPの同時解析2010

    • Author(s)
      安田年博, 他
    • Organizer
      日本DNA多型学会
    • Place of Presentation
      三島市文化会館(静岡県)
    • Year and Date
      2010-11-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Simultaneous genotyping of ten non-synonymous SNPs in the three human SOD genes2009

    • Author(s)
      R. Iida et al
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Simultaneous genotyping of ten non-synonymous SNPs in the three human SOD genes2009

    • Author(s)
      飯田礼子, 他
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] DNASE I exon内SNP検索:アジア人においてDNase I Gln222Argのみが多型性を有する2009

    • Author(s)
      藤原純子, 他
    • Organizer
      日本法医学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2009-05-15
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2019-07-29  

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