Metabolome analysis in the gastric carcinogenesis induced by the cross-talk between Helicobacter pylori and host.

• Project/Area Number: 21390228
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kobe University
• Principal Investigator: AZUMA Takeshi 神戸大学, 医学研究科, 教授 (60221040)
• Co-Investigator(Kenkyū-buntansha): YOSHINO Ken-ichi 神戸大学, バイオシグナル研究センター, 助教 (90280792)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: H. pylori / プロテオミクス解析 / メタボロミクス解析 / H.pylori

Research Abstract

The complete genome sequences of four Helicobacter pylori strains isolated from Japan were determined. A phylogenetic tree of concatenated well-defined core genes supported divergence of the East Asian lineage from the European lineage ancestor, and then from the Amerind lineage ancestor. Phylogenetic profiling revealed a large difference in the repertoire of outer membrane proteins. The results define H. pylori East Asian lineages and provide essential information for understanding their pathogenesis.
We investigated metabolome analysis using liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry. Lactic acid was upregulated in H. pylori infected AGS(gastric cancer cell line) cells and serum of gastric cancer patients. In contrast, malic acid was downregulated in H. pylori infected AGS cells and serum of gastric cancer patients.

Research Products

• [Journal Article] IFN-γplays an essential role in the pathogenesis of gastric lymphoid follicles formation caused by Helicobacter suis infection (2011)
  • Author(s): Mimura T, Yoshida M, Nishiumi S, Tanaka H, Nobutani K, Takenaka M, Suleiman YB, Yamamoto K, Ota H, Takahashi S, Matsui H, Nakamura M, Miki I, Azuma T
  • Journal Title: FEMS Immunol Med Microbiol、FEMS Immunol Med Microbiol Volume: 63(1) Pages: 25-34
  • Peer Reviewed
• [Journal Article] Evolution in an oncogenic bacterial species with extreme genome plasticity : Helicobacter pylori East Asian genomes (2011)
  • Author(s): Kawai M, Furuta Y, Yahara K, Tsuru T, Oshima K, Handa N, Takahashi N, Yoshida M, Azuma T, Hattori M, Uchiyama I, Kobayashi I
  • Journal Title: BMC Microbiol Volume: 11 Pages: 104-104
  • Peer Reviewed
• [Journal Article] Helicobacter suis KB1 derived from pig gastric lymphoid follicles induces the formation of gastric lymphoid follicles in mice through the activation of B cells and CD4 positive cells (2011)
  • Author(s): Yamamoto K, Tanaka H, Nishitani Y, Nishiumi S, Miki I, Takenaka M, Nobutani K, Mimura T, Ben Suleiman Y, Mizuno S, Kawai M, Uchiyama I, Yoshida M, Azuma T
  • Journal Title: Microbes Infect Volume: 13(7) Pages: 697-708
  • Peer Reviewed
• [Journal Article] Birth and death of genes linked to chromosomal inversion (2011)
  • Author(s): Furuta Y, Kawai M, Yahara K, Takahashi N, Handa N, Tsuru T, Oshima K, Yoshida M, Azuma T, Hattori M, Uchiyama I, Kobayashi I
  • Journal Title: Proc Natl Acad Sci USA Volume: 108(4) Pages: 1501-1506
  • Peer Reviewed
• [Journal Article] INF-γ plays an essential role in the pathogenesis of gastric lymphoid follicles formation caused by Helicobacter suis infection (2011)
  • Author(s): Mimura T, et al
  • Journal Title: FEMS Immunol Med Microbiol Volume: 63 Pages: 25-34
  • Peer Reviewed
• [Journal Article] Evolution in an oncogenic bacterial species with extreme genome plasticity : Helicobacter pylori East asian genomes (2011)
  • Author(s): Kawai M, et al
  • Journal Title: BMC Microbiol Volume: 11 Pages: 104-104
  • Peer Reviewed
• [Journal Article] Helicobacter suis KB1 derived from pig gastric lymphoid follicles induces the formation of gastric lymphoid follicles in mice through the activation of B cells and CD4 positive cells (2011)
  • Author(s): Yamamoto K, et al
  • Journal Title: Microbes Infect Volume: 13 Pages: 697-708
  • Peer Reviewed
• [Journal Article] Birth and death of genes linked to chromosomal inversion. (2011)
  • Author(s): Furuta K, et al.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 108(4) Pages: 1501-1506
  • Peer Reviewed
• [Journal Article] Helicobacter heilmannii can induce gastric lymphoid follicles in mice via a Peyer's patch-independent pathway (2010)
  • Author(s): Nobutani K, Yoshida M, Nishiumi S, Nishitani Y, Takagawa T, Tanaka H, Yamamoto K, Mimura T, Bensuleiman Y, Ota H, Takahashi S, Matsui H, Nakamura M, Azuma T
  • Journal Title: FEMS Immunol Med Microbiol Volume: 60(2) Pages: 156-164
  • Peer Reviewed
• [Journal Article] Diversity of the cagA gene of Helicobacter pylori strains from patients with gastroduodenal diseases in the Philippines (2010)
  • Author(s): Cortes MC, Yamakawa A, Casingal CR, Fajardo LS, Juan ML, De Guzman BB, Bondoc EM ; St. Mahachai V, Yamazaki Y, Yoshida M, Kutsumi H, Natividad FF, Azuma T
  • Journal Title: FEMS Immunol Med Microbiol Volume: 60(1) Pages: 90-97
  • Peer Reviewed
• [Journal Article] The CagA protein of Helicobacter pylori suppresses the functions of dendritic cell in mice (2010)
  • Author(s): Tanaka H, Yoshida M, Nishiumi S, Ohnishi N, Kobayashi K, Yamamoto K, Fujita T, Hatakeyama M, Azuma T
  • Journal Title: Arch Biochem Biophys Volume: 498(1) Pages: 35-42
  • NAID: 120003307586
  • Peer Reviewed
• [Journal Article] Prescreening of a high-risk group for gastric cancer by serologically determined Helicobacter pylori infection and atrophic gastritis (2010)
  • Author(s): Mizuno S, Miki I, Ishida T, Yoshida M, Onoyama M, Azuma T, Habu Y, Inokuchi H, Ozasa K, Miki K, Watanabe Y
  • Journal Title: Dig Dis Sci Volume: 55(11) Pages: 3132-3137
  • Peer Reviewed
• [Journal Article] Infection of less virulent Helicobacter pylori strains in asymptomatic healthy individuals in Thailand as a potential contributing factor to the Asian enigma (2010)
  • Author(s): Hirai I, Sasaki T, Kimoto A, Yamamoto Y, Azuma T, Mahachai V, Hansomburana P, Lertkupinit C, Luangjaru S, Noophan P, Chanatrirattanapan R, Piyanirandr V, Sappajit T, Suthivarakom K, Sangsuk L, Wangroongsarb P
  • Journal Title: Microbes Infect Volume: 12(3) Pages: 227-230
  • Peer Reviewed
• [Journal Article] The CagA protein of Helicobacter pylori suppresses the functions of dendritic cell in mice. (2010)
  • Author(s): Tanaka H, et al.
  • Journal Title: Arch Biochem Biophys. Volume: 498(1) Pages: 35-42
  • Peer Reviewed
• [Journal Article] Diversity of the cagA gene of Helicobacter pylori strains from patients 1 with gastroduodenal diseases in the Philippines. (2010)
  • Author(s): Cortes MCC, et al.
  • Journal Title: FEMS Immunol Med Microbiol. Volume: 60(1) Pages: 90-7
  • Peer Reviewed
• [Journal Article] Prescreening of a High-Risk Group for Gastric Cancer by Serologically Determined Helicobacter pylori Infection and Atrophic Gastritis. (2010)
  • Author(s): Mizuno S, et al.
  • Journal Title: Dig Dis Sci. Volume: 55(11) Pages: 3132-7
  • Peer Reviewed
• [Journal Article] Helicobacter heilmannii can induce gastric lymphoid follicles in mice via a Peyer's patch-independent pathway. (2010)
  • Author(s): Nobutani K, et al.
  • Journal Title: FEMS Immunol Med Microbiol. Volume: 60(2) Pages: 156-164
  • Peer Reviewed
• [Journal Article] Diverse characteristics of the CagA gene of Helicobacter pylori strains collected from patients from southern vietnam with gastric cancer and peptic ulcer (2009)
  • Author(s): Truong BX, et al.
  • Journal Title: J Clin Microbiol 47(12) Pages: 4021-4028
  • Peer Reviewed
• [Journal Article] Enhanced expression of CCL20 in human Helicobacter pylori-associated gastritis (2009)
  • Author(s): Yoshida A, et al.
  • Journal Title: Clin Immunol 130(3) Pages: 290-297
  • Peer Reviewed
• [Journal Article] Infection of less virulent Helicobacter pylori strains in asymptomatic healthy individuals in Thailand as a potential contributing factor to the Asian enigma
  • Author(s): Hirai I, et al.
  • Journal Title: Microbes Infect (in press, 掲載確定)
  • Peer Reviewed
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/gi/index.htm
• [Patent(Industrial Property Rights)] メタボローム解析手法を用いた特定疾患の検査方法 (2010)
  • Inventor(s): 吉田優、西海信、東健
  • Industrial Property Rights Holder: 神戸大学
  • Industrial Property Number: 2010-010164
  • Filing Date: 2010-04-27