| Project/Area Number |
21390230
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kumamoto University |
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Principal Investigator |
SASAKI Yutaka 熊本大学, 大学院・生命科学研究部, 教授 (70235282)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKI Norie 熊本大学, 大学院・生命科学研究部, 准教授 (80253722)
NAGAHAMA Hiroyasu 熊本大学, 医学部附属病院, 助教 (60381000)
FUJIMOTO Jiro 兵庫医科大学, 医学部, 教授 (90199373)
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| Research Collaborator |
NAOE Hideaki 熊本大学, 医学部附属病院, 助教 (30599246)
WATANABE Takehisa 熊本大学, 大学院・生命科学研究部, 助教 (20634843)
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| Project Period (FY) |
2009 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2009: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Keywords | プロテオミクス / 翻訳後修飾 / 肝癌 / 治療抵抗性 / ﾌﾟﾛﾃｵﾐｸｽ / 細胞死抵抗性 / 遺伝子解析 / 遺伝子解 |
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| Research Abstract |
Although curative treatment, including surgical resection and radio frequency ablation (RFA), isperformed against hepatocellular carcinoma (HCC) in the clinical setting, annual recurrence rate hasbeen reported to be 15-10%, indicating that HCC might be categorized into cancers with worst prognosis.To overcome this issue, this study is aimed to identify key-molecules responsible for treatmentresistance and develop a new therapeutic strategy for HCC. In this context, we have performedcomprehensive analysis of gene and protein expression in hepatoma cells under apoptosis stimulation. Inaddition, post-translational modification is analyzed comprehensively . Consequently , around thirtyproteins are narrowed down, because phosphorylation status, one of the post-translational modifications,of these proteins is significantly changed under apoptosis stimulation. These proteins are includingchaperon proteins, cytoskeleton -related molecules. Functional analysis is currently being performed onthese proteins in order to identify key-molecules which are responsible for treatment resistance ofHCCs.
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