| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Research Abstract |
Hepatitis C virus(HCV) infection causes chronic liver diseases and is a worldwide health problem. Despite the increasing demand for the knowledge of viral replication and pathogenesis, detail analysis of viral life cycle has been hampered by the lack of efficient viral culture system. In this study, we aimed to establish genotype1b HCV culture system since genotype1b infection is most prevalent and resistant for interferon therapy. We isolate a new genotype1b HCV clone and constructed full-length cDNA of viral genome. By introduction of adaptive mutants, we could observe viral replication and infectious virus particle formation and secretion. We also constructed chimeric virus using genotype 1b and 2a. With this chimeric virus constructs, we analyzed the anti-viral sensitivity profiles. In conclusion, genotype1b HCV culture system is useful and important for anti-viral development and analysis of drug resistant profile of HCV.
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