| Project/Area Number |
21390241
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Yasuhiro 山口大学, 大学院・医学系研究科, 助教 (00260349)
YANO Masafumi 山口大学, 医学部附属病院, 講師 (90294628)
YAMAMOTO Takeshi 山口大学, 大学院・医学系研究科, 助教 (50363122)
UEYAMA Tsuyoshi 山口大学, 医学部附属病院, 助教 (60380010)
河村 修二 山口大学, 医学部附属病院, 助教 (10380007)
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| Research Collaborator |
宮崎 要介 山口大学, 大学院, 学生
青山 英和 山口大学, 大学院, 学生
白石 宏造 山口大学, 大学院, 学生
西野 静香 山口大学, 技術, 補佐員
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2009: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | protein phosphatase 1β / adeno-associated virus vector / gene therapy / RNAi / B-type natriuretic peptide promoter / AAVベクター / 心不全 / RNA干渉 / 遺伝子導入 / アデノ随伴ウイルスベクター / RNAi干渉 |
|---|
| Research Abstract |
The targeting of Ca^<2+> cycling has emerged as a potential therapy for the treatment of severe heart failure. Here, we developed a novel heart-failure-inducible gene transfer system that inhibits PP1 by adeno-associated virus vector 9(AAV9)-mediated shRNA. AAV9 mediated gene transfer mitigated progressive left ventricular remodeling and ameliorated heart failure progression in muscle LIM proteindeficient(MLPKO) mice cardiomyopathy. Heart failure inducible gene therapy system provides new potential as a strategy of treatment of severe heart failure and intractable fetal arrhythmia.
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