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Analysis of cancer metastasis using circulating tumor cells purified by micro-fluidics techniques

Research Project

Project/Area Number 21390257
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionNagoya University

Principal Investigator

HASEGAWA Yoshinori  名古屋大学, 大学院・医学系研究科, 教授 (20270986)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Maozumi  名古屋大学, 医学部附属病院, 助教 (30378020)
KONDO Masashi  名古屋大学, 医学部附属病院, 講師 (00378077)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2009: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords循環血液中癌細胞 / マイクロフルイディクス / 肺癌 / 上皮間葉系細胞転換 / EMT関連遺伝子 / ZEB1 / Twist遺伝子 / 癌転移 / 上皮細胞接着分子
Research Abstract

To investigate the mechanisms and the search of the molecular targets for lung cancer metastasis, we developed the methods for purifying the circulating tumor cells(CTC) from peripheral blood, and we created the prototype device using the nanopillar technique. We also investigated the genetic profiles of CTC, and selected the genes, which were characteristic for CTC. EMT regulatory molecules of ZEB1 and Twist will become molecular targets for lung cancer metastasis.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (36 results)

All 2011 2010 2009

All Journal Article (27 results) (of which Peer Reviewed: 26 results) Presentation (8 results) Book (1 results)

  • [Journal Article] Transient but not stable ZEB1knockdown dramatically in hibits growth of malignant pleural me sothelioma cells2011

    • Author(s)
      Horio M, Hasegawa Y, et al.(15人中15番目)
    • Journal Title

      Ann Surg Oncol

      Volume: Nov16

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clinical characteristics of Pneumocystis peneumonia in non-HIV patients and prognostic factors including microbiological genotypes BMC2011

    • Author(s)
      Matumura Y, Hasegawa Y, et al.(11人中10番目)
    • Journal Title

      Infectious Diseases

      Volume: 11 Pages: 76-76

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phase II study of S-1monotherapy as a first-line treatment for elderly patients with advanced nonsmall-cell lung cancer2011

    • Author(s)
      Nishiyama O, Hasegawa Y, et al.(14人中12番目)
    • Journal Title

      Anti-Cancer Drugs

      Volume: 22 Pages: 811-816

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] ARHGAP18, a GT Pase-activating protein for Rho A, controls cell shape, spreading, and motility2011

    • Author(s)
      Maeda M, Hasegawa Y, et al.(11人中9番目)
    • Journal Title

      Mol Biol Cell

      Volume: 22 Pages: 3840-52

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Attenuation of transforming growth factor-β-stimulated collagen production in fibroblasts by quercetin-induced hemeoxygenase-12011

    • Author(s)
      Nakamura T, Hasegawa Y, et al.(9人中8番目)
    • Journal Title

      Am J Respir Cell Mol Biol

      Volume: 44 Pages: 614-20

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Capsaicinoids regulate airway anion transporters through Rhokinase-and cyclic AMP-dependent mechanisms2011

    • Author(s)
      Hibino Y, Hasegawa Y, et al.(11人中11番目)
    • Journal Title

      Am J Respir Cell Mol Biol

      Volume: 45 Pages: 684-91

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mesenchymal stem cells stably transduced with a dominant-negative inhibitor of CCL2 greatly attenuate bleomycin-induced lung damage2011

    • Author(s)
      Saito S, Hasegawa Y, et al.(10人中9番目)
    • Journal Title

      Am J Pathol

      Volume: 179 Pages: 1088-94

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A clinical trial for the rapeuticdrug monitoring using microchip-basedfluo rescence polarizatio nimmunoassay2011

    • Author(s)
      Tachi T, Hasegawa Y, et al.(10人中9番目)
    • Journal Title

      Anal Bioanal Chem

      Volume: 401 Pages: 2301-5

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pivotal role of epithelial cell adhesion molecule in the survival of lung cancer cells2011

    • Author(s)
      Hase T, Hasegawa Y, et al.(14人中14番目)
    • Journal Title

      Cancer Sci

      Volume: 102 Pages: 1493-500

    • NAID

      10030242101

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pivotal role of epithelial cell adhesion molecule in the survival of lung cancer cells2011

    • Author(s)
      Hase T, et al
    • Journal Title

      Cancer Sci

      Volume: 102 Issue: 8 Pages: 1493-1500

    • DOI

      10.1111/j.1349-7006.2011.01973.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A clinical trial for therapeutic drug monitoring using microchip-based fluorescence polarization immunoassay2011

    • Author(s)
      Tachi T, et al
    • Journal Title

      Anal Bioanal Chem

      Volume: 401 Issue: 7 Pages: 2301-2305

    • DOI

      10.1007/s00216-011-5304-9

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ARHGAP18, a GTPase-activating protein for RhoA, controls cell shape, spreading, and motility2011

    • Author(s)
      Maeda M, et al
    • Journal Title

      Mol Biol Cell

      Volume: 22 Issue: 20 Pages: 3840-52

    • DOI

      10.1091/mbc.e11-04-0364

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pharma cokinetic analysis of carboplatin in patients with cancer who are undergoing hem dialysis]2010

    • Author(s)
      Oguri T, Hasegawa Y, et al.(7人中7番目)
    • Journal Title

      Cancer Chemoth Pharm

      Volume: 66 Pages: 813-7

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prospective evaluation of pharmacokinetic ally guided dosing of carboplatin in Japanese patients with cancer2010

    • Author(s)
      Shimokata T, Hasegawa Y, et al.(10人中10番目)
    • Journal Title

      Cancer Sci

      Volume: 101 Pages: 2601-5

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Central Japan Lung Study Group. Phase II study of carboplatin and gemcitabine as adjuvant chemotherapy in patients with completely resectednon-small cell lung cancer2010

    • Author(s)
      Usami N, Hasegawa Y, et al.(10人中3番目)
    • Journal Title

      CJLSG trial. Int J Clin Oncol

      Volume: 15 Pages: 583-7

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Heterogous regulation of anion transporters by menthol in human airway epithelial cells2010

    • Author(s)
      Morise M, Hasegawa Y, et al.(10人中10番目)
    • Journal Title

      Eur J Pharmacol

      Volume: 635 Pages: 204-11

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Endothelial-mesenchymal transition in bleomycin-inducedpulmonary fibrosis2010

    • Author(s)
      Hashimoto N, Hasegawa Y, et al.(8人中8番目)
    • Journal Title

      Am J Resp Cell Mol Biol

      Volume: 43 Pages: 161-72

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Actin cytoskeleton regulates stretch-activated Ca2+ influx inhuman pulmonary microvascular endothelial cells2010

    • Author(s)
      Ito S, Hasegawa Y, et al.(10人中9番目)
    • Journal Title

      Am J Resp Cell Mol Biol

      Volume: 43 Pages: 26-34

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serum KL-6 in fibrotic NSIP2010

    • Author(s)
      Sakamoto K, Hasegawa Y, et al.(12人中12番目)
    • Journal Title

      Resp Med

      Volume: 104 Pages: 127-133

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Knockdown of ZEB1, a master epithelial-to-mesenchymal transition(EMT) gene2010

    • Author(s)
      Takeyama Y, Hasegawa Y, et al.(13人中13番目)
    • Journal Title

      Cancer Letters

      Volume: 296 Pages: 216-24

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Knockdown of ZEB1, a master epithelial-to-mesenchymal transition (EMT) gene, suppresses anchorage-independent cell growth of lung cancer cells.2010

    • Author(s)
      Takeyama Y, Hasegawa Y, et al
    • Journal Title

      Cancer Letter

      Volume: 296 Pages: 216-24

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Endothelial-mesenchymal transition in bleomycin-induced pulmonary fibrosis.2010

    • Author(s)
      Hashimoto N, Hasegawa Y, et al.
    • Journal Title

      Am J Respir Cell Mol Biol

      Volume: 43 Pages: 161-72

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heterologous regulation of anion transporters by menthol in human airway epithelial cells.2010

    • Author(s)
      Morise M, Hasegawa Y, et al.
    • Journal Title

      Eur J Pharmacol

      Volume: 635 Pages: 204-11

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Up-regulation of surfactant protein production in a mouse model of secondary pulmonary alveolar proteinosis2009

    • Author(s)
      Shibasaki M, 他
    • Journal Title

      Am J Respir Cell Mol Biol 40

      Pages: 536-542

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A phase II study of carboplatin and paclitacel with meloxicam2009

    • Author(s)
      Suzuki R, 他
    • Journal Title

      Lung Cancer 63

      Pages: 72-76

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Erythromycin-induced CXCR4 expression on microvascular endothelial cells2009

    • Author(s)
      Takagi Y, 他
    • Journal Title

      Am J Physiol Lung Cell Mol Physiol 297

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      長谷川好規
    • Journal Title

      フレイザー 呼吸器病学 エッセンス(清水英治、藤田次郎 監訳)(西村書店)

      Pages: 199-235

    • Related Report
      2009 Annual Research Report
  • [Presentation] The circadian clock gene BMAL1 asapossible therapeutic target for malignant pleural mesothelioma2011

    • Author(s)
      Momen A, Hasegawa Y, et al.(10人10番目)
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report
  • [Presentation] Transient But Not Stable ZEB1Knockdown Dramatically Inhibits Growth Of Malignant Pleural Mesothelioma Cells2011

    • Author(s)
      Horio M, Hasegawa Y, et al.(11人11番目)
    • Organizer
      Implication Of Epcam Up-Regulation Induced By ZEB1 Knockdown ILSLC 14TH WORLD CONFERENCEON LUNG CANCER. Amsterdom
    • Place of Presentation
      The Netherlands
    • Related Report
      2011 Final Research Report
  • [Presentation] The Functional Role Of Epithelial Cell Adhesion Molecule(EPCAM) IN The Non-Small Cell Lung Cancer Cell Lines ILSLC2011

    • Author(s)
      Kondo M, Hasegawa Y, et al.(10人10番目)
    • Organizer
      14TH WORLD CONFERENCE ON LUNGCANCER. Amsterdom
    • Place of Presentation
      The Netherlands
    • Related Report
      2011 Final Research Report
  • [Presentation] Inhibition Of TGF-Beta-InducedPhenotype Alterations ThroughEpithelial-MesenchymalTransition(EMT) In Lung Cancer By GeneModulation Of Phosphorylations SitesIn Tumor Suppressor Pten2011

    • Author(s)
      Aoyama D, Hasegawa Y, et al.(7人7番目)
    • Organizer
      American Thoracic Society International Conference 2011 Denver
    • Place of Presentation
      Colorado, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Gene Modulation Of Phosphorylation Sites In Tumor Suppressor PTEN Inhibits Hypoxia-Induced Phenotype ChangesThrough Epithelial-MesenchymalTransition(EMT) In Lung Cancer2011

    • Author(s)
      Kohnoh T, Hasegawa Y, et al.(7人7番目)
    • Organizer
      American Thoracic Society International Conference 2011(ATS・2011)
    • Place of Presentation
      Denver, Colorado, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Modulated Expression Of Surfactant Protein D In Acute Lung Injury Might Be Associated With Epithelial-Mesenchymal Transition In Epithelial Cells2011

    • Author(s)
      Sakamoto K, Hasegawa Y, et al.(9人9番目)
    • Organizer
      American Thoracic Society International Conference 2011(ATS・2011)
    • Place of Presentation
      Denver, Colorado, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Transient but not stable ZEB1 knockdown dramatically inhibits growth of malignant pleural mesothelioma cells : implication of Epcam up-regulation induced by ZEB1 knockdown2011

    • Author(s)
      Horio M
    • Organizer
      ILSLC 14TH WORLD CONFERENCE ON LUNG CANCER
    • Place of Presentation
      Amsterdom, Netherlands
    • Related Report
      2011 Annual Research Report
  • [Presentation] Knockdown of ZEB1, a master EMT gene, suppresses growth of pleural mesothelioma cell lines.2010

    • Author(s)
      Sato M, Hasegawa Y, et al.
    • Organizer
      The 10^<th> International Conference of the International Mesothelioma Interest Group
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2010 Annual Research Report
  • [Book] 抗悪性腫瘍薬コンサルトブック2010

    • Author(s)
      南博信編集
    • Total Pages
      34
    • Publisher
      南江堂
    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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