| Project/Area Number |
21390283
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Research Institute, International Medical Center of Japan |
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Principal Investigator |
YASUDA Kazuki 独立行政法人国立国際医療研究センター, 研究所 糖尿病研究センター 代謝疾患研究部, 部長 (80311611)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMURA Tadashi 国立国際医療研究センター, 研究所, 室長 (00333790)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2009: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | 糖尿病 / 遺伝子 / 発現調節 / RNA / 膵β細胞 |
|---|
| Research Abstract |
Insulin producing pancreatic β-cells are highly differentiated tissue, In order to elucidate molecular mechanisms of transcriptional regulation in β-cells, we constructed a novel full-length cDNA library by Vector-capping method from rat β-cell line INS-1D cells, and performed a 5'-one pass shotgun sequencing with 26,976 clones, resulting in 6,042 clusters. We identified novel transcriptional start sites (TSSs) and possible transcriptional regulatory regions for some genes. We also obtained several functional non-coding RNA (ncRNA) candidates, which may be involved in insulin secretion. Thus, our system should serve as a novel tool for investigating 'RNA world' of pancreatic β-cells.
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