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A study on regulatory genomic regions and functional RNAs in pancreatic β-cells

Research Project

Project/Area Number 21390283
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionResearch Institute, International Medical Center of Japan

Principal Investigator

YASUDA Kazuki  独立行政法人国立国際医療研究センター, 研究所 糖尿病研究センター 代謝疾患研究部, 部長 (80311611)

Co-Investigator(Kenkyū-buntansha) OKAMURA Tadashi  国立国際医療研究センター, 研究所, 室長 (00333790)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2009: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Keywords糖尿病 / 遺伝子 / 発現調節 / RNA / 膵β細胞
Research Abstract

Insulin producing pancreatic β-cells are highly differentiated tissue, In order to elucidate molecular mechanisms of transcriptional regulation in β-cells, we constructed a novel full-length cDNA library by Vector-capping method from rat β-cell line INS-1D cells, and performed a 5'-one pass shotgun sequencing with 26,976 clones, resulting in 6,042 clusters. We identified novel transcriptional start sites (TSSs) and possible transcriptional regulatory regions for some genes. We also obtained several functional non-coding RNA (ncRNA) candidates, which may be involved in insulin secretion. Thus, our system should serve as a novel tool for investigating 'RNA world' of pancreatic β-cells.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (4 results)

All 2012 2011

All Journal Article (1 results) Presentation (1 results) Book (2 results)

  • [Journal Article] Regulatory RNAと糖尿病2012

    • Author(s)
      安田和基
    • Journal Title

      最新医学

      Volume: 67巻1号

    • Related Report
      2011 Final Research Report
  • [Presentation] 遺伝因子からみた2型糖尿病の成因2011

    • Author(s)
      安田和基
    • Organizer
      第28回日本医学会総会
    • Place of Presentation
      (東日本大震災のためデータ登録)
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Book] 「Non-coding RNAによる代謝調節」糖尿病学イラストレイテッド2012

    • Author(s)
      南茂隆生、安田和基
    • Publisher
      羊土社
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Book] 「Regulatory RNAと糖尿病」最新医学67巻1号2012

    • Author(s)
      安田和基
    • Total Pages
      7
    • Publisher
      最新医学社
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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