|Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
GATA1 is a transcription factor essential for erythroid/megakaryocytic cell differentiation. We found that three elements within promoter-proximal enhancer differentially contribute to spatio-temporal expression of the Gata1 gene in vivo. In addition, we found that loss of the GATA1-FOG1 interaction causes a unique combination of membrane protein deficiency, leading to the hemolysis. We also found that GATA1 has two independent transactivation domains, N-TAD and C-TAD, and both retain redundant as well as specific activities for proper hematopoiesis in vivo.