Development of gene therapy using bone-marrow-derived mesenchymal stem cells

• Project/Area Number: 21390296
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Jichi Medical University
• Principal Investigator: OZAWA Keiya 自治医科大学, 医学部, 教授 (30137707)
• Co-Investigator(Kenkyū-buntansha): URABE Masashi 自治医科大学, 医学部, 講師 (40213516) ; UCHIBORI Ryosuke 自治医科大学, 医学部, 助教 (20458285)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 間葉系幹細胞 / 癌 / 遺伝子治療 / 移植再生医療 / ゲノム

Research Abstract

The development of gene therapy technologies using mesenchymal stem cells(MSCs) was conducted. First, MSCs have the ability to accumulate at tumor sites, and we investigated the mechanisms using tumor-bearing nude mice. As a result, important information was obtained that will be useful when MSCs are utilized as a platform for cancer-targeted gene therapy. Second, in order to ensure the safety of genetic manipulation of MSCs, we conducted basic studies on the site-specific integration of transgenes using adeno-associated virus integration machinery for the AAVS1 locus on chromosome 19.

Research Products

• [Journal Article] Interleukin-10 expression induced by adeno-associated virus vector suppresses proteinuria in Zucker obese rats (2012)
  • Author(s): Ogura M, Urabe M, Akimoto T, Onishi A, Ito C, Ito T, Tsukahara T, Mizukami H, Kume A, Muto S, Kusano E, and Ozawa K.
  • Journal Title: Gene Ther Volume: 19巻 Issue: 5 Pages: 476-82
  • DOI: 10.1038/gt.2011.183
  • Peer Reviewed
• [Journal Article] Recovery of neurogenic amines in phenylketonuria mice after liver-targeted gene therapy (2012)
  • Author(s): Yagi H, Sanechika S, Ichinose H, Sumi-Ichinose C, Mizukami H, Urabe M, Ozawa K, Kume A
  • Journal Title: Neuroreport Volume: 4巻 Pages: 30-4
  • Peer Reviewed
• [Journal Article] Indoleamine-2,3-dioxygenase, an immunosuppressive enzyme that inhibits natural killer cell function, as a useful target for ovarian cancer therapy (2012)
  • Author(s): Wang, D.
  • Journal Title: Int J Oncol Volume: 40 Issue: 4 Pages: 929-34
  • DOI: 10.3892/ijo.2011.1295
  • Peer Reviewed
• [Journal Article] Morphologic analysis in myelodysplastic syndromes with del(5q) treated with lenalidomide. A Japanese multiinstitutional study (2012)
  • Author(s): Matsuda, A.
  • Journal Title: Leuk Res Volume: 36 Pages: 575-80
  • Peer Reviewed
• [Journal Article] Recovery of neurogenic amines in phenylketonuria mice after liver-targeted gene therapy (2012)
  • Author(s): Yagi, H.
  • Journal Title: Neuroreport Volume: 23 Pages: 30-4
  • Peer Reviewed
• [Journal Article] Development of a mouse model for lymph node metastasis with endometrial cancer (2011)
  • Author(s): Takahashi K, Saga Y, Mizukami H, Takei Y, Urabe M, Kume A, Suzuki M, and Ozawa K
  • Journal Title: Cancer Sci Volume: 102巻 Issue: 12 Pages: 2272-7
  • DOI: 10.1111/j.1349-7006.2011.02099.x
  • NAID: 10030246395
  • Peer Reviewed
• [Journal Article] Lack of IL-21 signal attenuates graft-versus-leukemia effect in the absence of CD8 T-cells (2011)
  • Author(s): Meguro A, Ozaki K, Hatanaka K, Oh I, Sudo K, Ohmori T, Matsu H, Tatara., Sato K, Sakata Y, Nakae S, Leonard W J, and Ozawa K
  • Journal Title: Bone Marrow Transplant Volume: 46巻 Issue: 12 Pages: 1557-1565
  • DOI: 10.1038/bmt.2010.342
  • Peer Reviewed
• [Journal Article] Mesenchymal stromal cells inhibit Th17 but not regulatory T-cell differentiation (2011)
  • Author(s): Tatara R, Ozak K., Kikuchi Y, Hatanaka K, Oh I, Meguro A, Matsu H, Sato K, and Ozawa K
  • Journal Title: Cytotherapy Volume: 13巻 Pages: 686-694
  • Peer Reviewed
• [Journal Article] Complete restoration of phenylalanine oxidation in phenylketonuria mouse by a self-complementary adeno-associated virus vector (2011)
  • Author(s): Yagi H, Ogura T, Mizukami H, Urabe M, Hamada H, Yoshikawa H, Ozawa K and Kume A
  • Journal Title: J. Gene Med Volume: 13巻 Issue: 2 Pages: 114-122
  • DOI: 10.1002/jgm.1543
  • Peer Reviewed
• [Journal Article] Vincristine potentiates the anti-proliferative effect of an aurora kinase inhibitor, VE-465, in myeloid leukemia cells (2011)
  • Author(s): Yoshida, K.
  • Journal Title: Biochem Pharmacol Volume: 82 Pages: 1884-1890
  • Peer Reviewed
• [Journal Article] Selective optical control of synaptic transmission in the subcortical visual pathway by activation of viral vector-expressed halorhodopsin (2011)
  • Author(s): Kaneda, K.
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] Mesenchymal stromal cells inhibit Th17 but not regulatory T-cell differentiation (2011)
  • Author(s): Tatara, R.
  • Journal Title: Cytotherapy Volume: 13 Pages: 686-694
  • Peer Reviewed
• [Journal Article] Altered effector CD4+T cell function in IL-21R-/-CD4+T cell-mediated graft-versus-host disease (2010)
  • Author(s): Oh I, Ozaki K, Meguro A, Hatanaka K, Kadowaki M, Matsu H, Tatara R, Sato K, Iwakura Y, Nakae S, Sudo K, Teshima T, Leonard W J and Ozawa K
  • Journal Title: J. Immunol Volume: 185巻 Pages: 1920-1926
  • Peer Reviewed
• [Journal Article] IL-21 is critical for GVHD in a mouse model (2010)
  • Author(s): Meguro A, Ozaki K, Oh I, Hatanaka K, Matsu H, Tatara R, Sato. K, Leonard W J and Ozawa K
  • Journal Title: Bone Marrow Transplant Volume: 45巻 Pages: 723-729
  • Peer Reviewed
• [Journal Article] Mutant Macaque Factor IX T262A : A Tool for Hemophilia B Gene Therapy Studies in Macaques (2010)
  • Author(s): Ishiwata A, Mimuro J, Mizukami H, Kashiwakura Y, Yasumoto A, Sakata A, Ohmori T, Madoiwa S, Ono F, Shima M, Yoshioka A, Ozawa K, Sakata Y
  • Journal Title: Thromb Res Volume: 125巻 Pages: 533-7
  • Peer Reviewed
• [Journal Article] Characterization of a Recombinant Adeno-Associated Virus Type 2 Reference Standard Material (2010)
  • Author(s): Lock M, McGorray S, Auricchio A, Ayuso E, Beecham E J, Blouin V, Bosch F, Bose M, Byrne B, Caton T, Chiorini J, Chtarto A, Clark K R, Conlon T, Darmon C, Doria M, Douar A M, Flotte T R, Francis J, Francois A, Giacca M., Korn M., Korytov I, Leon X, Leuchs
  • Journal Title: Human Gene Therapy Volume: 21巻 Pages: 1723-85
  • Peer Reviewed
• [Journal Article] A phase I study of aromatic L-amino acid decarboxylase gene therapy for Parkinson's disease (2010)
  • Author(s): Muramatsu S, Fujimoto K, Kato S, Mizukami H, Asari S, Ikeguchi K, Kawakami T, Urabe M, Kume A, Sato T, Watanabe E, Ozawa K, Nakano I
  • Journal Title: Mol Ther Volume: 18巻 Pages: 1731-5
  • Peer Reviewed
• [Journal Article] Gene therapy for Parkinson's disease. Strategies for the local production of dopamine (2010)
  • Author(s): Muramatsu S, Asari S, Fujimoto K, Ozawa K and Nakamo I.
  • Journal Title: Gene Therapy & Regulation Volume: 5巻 Pages: 57-65
  • Peer Reviewed
• [Journal Article] Establishment and characterization of transplantable, luminescence labeled rat renal cell carcinoma cell lines (2010)
  • Author(s): Kobayashi M, Murakami T, Uchibori R, Chun NA, Kobayashi E, Morita T, Ozawa K.
  • Journal Title: J Urol Volume: 183巻 Pages: 2029-2035
  • Peer Reviewed
• [Journal Article] Altered effector CD4+ T cell function in IL-21R-/- CD4+ T cell-mediated graft-versus-host disease. (2010)
  • Author(s): Oh, I.
  • Journal Title: J.Immunol. Volume: 185 Pages: 1920-1926
  • Peer Reviewed
• [Journal Article] Histone deacetylases are critical targets of bortezomib-induced cytotoxicity in multiple myeloma. (2010)
  • Author(s): Kikuchi, J.
  • Journal Title: Blood Volume: 116 Pages: 406-417
  • Peer Reviewed
• [Journal Article] FOXO3A as a key molecule for all-trans retinoic acid-induced granulocytic differentiation and apoptosis in acute promyelocytic leukemia. (2010)
  • Author(s): Sakoe, Y.
  • Journal Title: Blood Volume: 115 Pages: 3787-3795
  • Peer Reviewed
• [Journal Article] IL-21 is critical for GVHD in a mouse model. (2010)
  • Author(s): Meguro, A.
  • Journal Title: Bone Marrow Transplant. Volume: 45 Pages: 723-729
  • Peer Reviewed
• [Journal Article] Mutant macaque factor IX T262A : a tool for hemophilia B gene therapy studies in macaques (2010)
  • Author(s): Ishiwata, A.
  • Journal Title: Thromb.Res. Volume: 125 Pages: 533-537
  • Peer Reviewed
• [Journal Article] Characterization of a recombinant adeno-associated virus type 2 Reference Standard Material (2010)
  • Author(s): Lock, M.
  • Journal Title: Hum.Gene Ther. Volume: 21 Pages: 1273-1285
  • Peer Reviewed
• [Journal Article] A phase I study of aromatic L-amino acid decarboxylase gene therapy for Parkinson's disease. (2010)
  • Author(s): Muramatsu, S.
  • Journal Title: Mol.Ther. Volume: 12 Pages: 1731-1735
  • Peer Reviewed
• [Journal Article] Gene therapy for Parkinson's disease. Strategies for the local production of dopamine. (2010)
  • Author(s): Muramatsu, S.
  • Journal Title: Gene Therapy & Regulation Volume: 5 Pages: 57-65
  • Peer Reviewed
• [Journal Article] Complete restoration of phenylalanine oxidation in phenylketonuria mouse by a self-complementary adeno-associated virus vector. (2010)
  • Author(s): Yagi, H.
  • Journal Title: J.Gene Med. Volume: 13 Pages: 114-122
  • Peer Reviewed
• [Journal Article] 間葉系幹細胞を用いたがん標的化療法 (2010)
  • Author(s): Uchibori, R.
  • Journal Title: 臨床血液 Volume: 51 Pages: 1641-6
  • NAID: 10027751339
  • Peer Reviewed
• [Journal Article] Establishment and characterization of transplantable, luminescence labeled rat renal cell carcinoma cell lines. (2010)
  • Author(s): Kobayashi, M.
  • Journal Title: J Urol Volume: 183 Pages: 2029-2035
  • Peer Reviewed
• [Journal Article] Liver-restricted expression of the canine factor VIII gene facilitates prevention of inhibitor formation in factor VIII-deficient mice (2009)
  • Author(s): Ishiwata A, Mimuro J, Mizukami H, Kashiwakura Y, Takano K, Ohmori T, Madoiwa S, Ozawa K and Sakata Y
  • Journal Title: J. Gene Med Volume: 11巻 Pages: 1020-1029
  • Peer Reviewed
• [Journal Article] Generation of adeno-associated virus vector enabling functional expression of oxytocin receptor and fluorescence marker genes using the human eIF4G internal ribosome entry site element (2009)
  • Author(s): Sato K, Date S, Aoyagi Y, Kasahara Y, Nawa A, Mizukami H, Hidema S, Ozawa K, Nishimori K
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 73巻 Pages: 2145-2148
  • NAID: 10027546295
  • Peer Reviewed
• [Journal Article] A convenient enzyme-linked immunosorbent assay for rapid screeningof anti-adeno-associated virus neutralizing antibodies (2009)
  • Author(s): Ito T, Yamamoto S, Hayashi T, Kodera M, Mizukami H, Ozawa K, Muramatsu S.
  • Journal Title: Ann. Clin. Biochem Volume: 46巻 Pages: 508-510
  • Peer Reviewed
• [Journal Article] Cotransplantation with MSCs improves engraftment of HSCs after autologous intra-bone marrow transplantation in nonhuman primates (2009)
  • Author(s): Masuda S, Ageyama N, Shibata H, Obara Y, Ikeda T, Takeuchi K, Ueda Y, Ozawa K., Hanazono Y
  • Journal Title: Exp. Hematol Volume: 37巻 Pages: 1250-1257
  • Peer Reviewed
• [Journal Article] Scalable purification of adeno-associated virus serotype 1(AAV1) and AAV8 vectors, using dual ion-exchange adsorptive membranes (2009)
  • Author(s): Okada T, Nonaka-Sarukawa M, Uchibori R, Kinoshita K, Hayashita-Kinoh H, Nitahara-Kasahara Y, Takeda S, and Ozawa K
  • Journal Title: Hum. Gene Ther Volume: 20巻 Pages: 1013-1021
  • Peer Reviewed
• [Journal Article] Retroviral vector-producing mesenchymal stem cells for targeted suicide cancer gene therapy (2009)
  • Author(s): Uchibori R, Okada T, Ito T, Mizukami H, Kume A and Ozawa, K
  • Journal Title: J. Gene Med Volume: 11巻 Pages: 373-381
  • Peer Reviewed
• [Journal Article] Systemic delivery of IL-10 by an AAV vector prevents vascular remodeling and end-organ damage in stroke-prone spontaneously hypertensive rat (2009)
  • Author(s): Nomoto T, Okada T, Shimazaki K, Yoshioka T, Nonaka-Sarukawa M, Ito T, Takeuchi K, Katsura K I, Mizukami H, Kume A, Ookawara S, Ikeda U, Katayama Y, and Ozawa K
  • Journal Title: Gene Ther Volume: 16巻 Pages: 383-91
  • Peer Reviewed
• [Journal Article] Liver-restricted expression of the canine factor VIII gene facilitates prevention of inhibitor formation in factor VIII-deficient mice. (2009)
  • Author(s): Ishiwata, A.
  • Journal Title: J.Gene Med. 11 Pages: 1020-1029
  • Peer Reviewed
• [Journal Article] Generation of adeno-associated virus vector enabling functional expression of oxytocin receptor and fluorescence marker genes using the human eIF4G internal ribosome entry site element. (2009)
  • Author(s): Sato, K.
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 2145-2148
  • Peer Reviewed
• [Journal Article] A convenient enzyme-linked immunosorbent assay for rapid screening of anti-adeno-associated virus neutralizing antibodies. (2009)
  • Author(s): Ito, T.
  • Journal Title: Ann.Clin.Biochem. 46 Pages: 508-510
  • Peer Reviewed
• [Journal Article] Cotransplantation with MSCs improves engraftment of HSCs after autologous intra-bone marrow transplantation in nonhuman primates. (2009)
  • Author(s): Masuda, S
  • Journal Title: Exp.Hematol. 37 Pages: 1250-1257
  • Peer Reviewed
• [Journal Article] Scalable purification of adeno-associated virus serotype 1(AAV1) and AAV8 vectors, using dual ion-exchange adsorptive membranes. (2009)
  • Author(s): Okada, T.
  • Journal Title: Hum.Gene Ther. 20 Pages: 1013-1021
  • Peer Reviewed
• [Journal Article] Retroviral vector-producing mesenchymal stem cells for targeted suicide cancer gene therapy. (2009)
  • Author(s): Uchibori, R.
  • Journal Title: J.Gene Med. 46 Pages: 373-381
  • Peer Reviewed
• [Journal Article] Systemic delivery of IL-10 by an AAV vector prevents vascular remodeling and end-organ damage in stroke-Drone spontaneously hypertensive rat. (2009)
  • Author(s): Nomoto, T.
  • Journal Title: Gene Ther. 16 Pages: 383-391
  • Peer Reviewed
• [Journal Article] Bortezomib overcomes cell adhesion-mediated drug resistance through downregulation of VLA-4 expression in multiple myeloma. (2009)
  • Author(s): Hatano, K.
  • Journal Title: Oncogene 28 Pages: 231-242
  • Peer Reviewed
• [Journal Article] Histone deacetylases are critical targets of bortezomib-induced cytotoxicity in multiple myeloma.
  • Author(s): Kikuchi, J.
  • Journal Title: Blood (in press)
  • Peer Reviewed
• [Journal Article] FOXO3A as a key molecule for all-trans retinoic acid-induced granulocytic differentiation and apoptosis in acute promyelocytic leukemia.
  • Author(s): Sakoe, Y.
  • Journal Title: Blood (in press)
  • Peer Reviewed
• [Journal Article] Mutant macaque factor IX T262A : A tool for hemophilia B gene therapy studies in macaques.
  • Author(s): Ishiwata, A.
  • Journal Title: Thromb.Res. (in press)
  • Peer Reviewed
• [Journal Article] Combination of tipifarnib and rapamycin synergistically inhibits the growth of leukemia cells and overcomes resistance to tipifarnib via alteration of cellular signaling pathways.
  • Author(s): Nagai, T.
  • Journal Title: Leuk.Res. (in press)
  • Peer Reviewed
• [Journal Article] IL-21 is critical for GVHD in a mouse model. Bone Marrow Transplant.
  • Author(s): Meguro, A.
  • Journal Title: Bone Marrow Transplant. (in press)
  • Peer Reviewed
• [Presentation] The adeno-associated virus p5 promoter sequence mediated integration of transgene into the AAVS1 site (2011)
  • Author(s): Urabe M
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-12-13
• [Presentation] Synergistic effect on the tumor-killing activity of soluble TRAIL-expressing MSCs (2011)
  • Author(s): Uchibori R
  • Organizer: 第70回に本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2011-10-04
• [Presentation] Synergistic effect on the tumor-killing activity of soluble TRAIL-expressing MSCs (2011)
  • Author(s): Uchibori, R.
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2011-10-04
• [Presentation] The p5 promoter of adeno-associated virus for AAVS1-specific integration (2011)
  • Author(s): Urabe M
  • Organizer: The XVth International Congress of Virology
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-13
• [Presentation] Relationship between neutralizing antibody and transgene expression in non-human primates following administration of AAV8 vectors (2011)
  • Author(s): Mizukami, H.
  • Organizer: The XVth International Congress of Virology
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-13
• [Presentation] The role of the p5 promoter of adeno-associated virus in AAVS1-directed integration (2011)
  • Author(s): Urabe M
  • Organizer: The 17th Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
  • Year and Date: 2011-07-17
• [Presentation] The role of the p5 promoter of adeno-associated virus in AAVS1-directed integration (2011)
  • Author(s): Urabe, M.
  • Organizer: The 17^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
  • Year and Date: 2011-07-17
• [Presentation] Consistent and robust factor IX expression in Nab-negative macaques by IV administration of AAV8 vectors (2011)
  • Author(s): Mizukami, H.
  • Organizer: The 17^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
  • Year and Date: 2011-07-17
• [Presentation] Trichostatin A sensitize apoptosis induced by soluble TRAIL-expressing MSCs (2011)
  • Author(s): Uchibori R
  • Organizer: The 17th Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
  • Year and Date: 2011-07-16
• [Presentation] Trichostatin A sensitize apoptosis induced by soluble TRAIL-expressing MSCs (2011)
  • Author(s): Uchibori, R.
  • Organizer: The 17^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
  • Year and Date: 2011-07-16
• [Presentation] Interleukin-10 prevents the progression of peritoneal fibrosis (2011)
  • Author(s): Onishi, A.
  • Organizer: The 17^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
  • Year and Date: 2011-07-15
• [Presentation] AADC gene therapy for Parkinson's disease : four years of follow-up (2011)
  • Author(s): Muramatsu, S.
  • Organizer: The 17^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
  • Year and Date: 2011-07-15
• [Presentation] The p5 promoter sequence of AAV genome regulates the direction of transgene insertion into the AAVS1 site (2011)
  • Author(s): Urabe M
  • Organizer: The 14th Annual Meeting of the American Society of Gene and Cell Therapy
  • Place of Presentation: The Washington State Convention Center(Seattle)
  • Year and Date: 2011-05-21
• [Presentation] The p5 promoter sequence of AAV genome regulates the direction of transgene insertion into the AAVS1 site (2011)
  • Author(s): Urabe, M.
  • Organizer: The 14^<th> Annual Meeting of the American Society of Gene and Cell Therapy
  • Place of Presentation: The Washington State Convention Center (Seattle)
  • Year and Date: 2011-05-21
• [Presentation] Enhancement of Tumor-Killing Activity of TRAIL-Expressing MSCs by Using Trichostatin A (2011)
  • Author(s): Uchibori R
  • Organizer: The 14th Annual Meeting of the American Society of Gene and Cell Therapy
  • Place of Presentation: The Washington State Convention Center(Seattle)
  • Year and Date: 2011-05-20
• [Presentation] Enhancement of Tumor-Killing Activity of TRAIL-Expressing MSCs by Using Trichostatin A (2011)
  • Author(s): Uchibori, R.
  • Organizer: The 14^<th> Annual Meeting of the American Society of Gene and Cell Therapy
  • Place of Presentation: The Washington State Convention Center (Seattle)
  • Year and Date: 2011-05-20
• [Presentation] Relationship between neutralizing antibody and factor IX expression in non-human primates following iv administration of AAV8 vectors (2011)
  • Author(s): Mizukami, H.
  • Organizer: The 14^<th> Annual Meeting of the American Society of Gene and Cell Therapy
  • Place of Presentation: The Washington State Convention Center (Seattle)
  • Year and Date: 2011-05-19
• [Presentation] 再生医療におけるゲノム改変技術 (2011)
  • Author(s): Urabe, M.
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2011-03-01
• [Presentation] Analysis of cis elements required for AAVS1-specific integration of adeno-associated virus (2010)
  • Author(s): Urabe M
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2010-12-07
• [Presentation] Analysis of cis elements required for AAVS1-specific integration of adeno-associated virus. (2010)
  • Author(s): Urabe, M.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2010-12-07
• [Presentation] アデノ随伴ウイルスの部位特異的組込み機構の解析 (2010)
  • Author(s): Urabe M
  • Organizer: 第58回日本ウイルス学会学術総会
  • Place of Presentation: あわぎんホ-ル(徳島)
  • Year and Date: 2010-11-09
• [Presentation] AAVに対する中和抗体検査法の改良とカニクイザルコロニーにおける陽性率 (2010)
  • Author(s): Mizukami, H.
  • Organizer: 第58回日本ウイルス学会学術総会
  • Place of Presentation: あわぎんホール(徳島)
  • Year and Date: 2010-11-09
• [Presentation] アデノ随伴ウイルスの部位特異的組込み機構の解析 (2010)
  • Author(s): Urabe, M.
  • Organizer: 第58回日本ウイルス学会学術総会
  • Place of Presentation: あわぎんホール(徳島)
  • Year and Date: 2010-11-09
• [Presentation] The effect of DMXAA(TNF-α inducer) on MSC accumulation at tumor sites (2010)
  • Author(s): Uchibori R
  • Organizer: 第16回日本癌学会総会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2010-09-24
• [Presentation] The effect of DMXAA (TNF-α inducer) on MSC accumulation at tumor sites. (2010)
  • Author(s): Uchibori, R.
  • Organizer: 第16回日本癌学会総会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2010-09-24
• [Presentation] Positron emission tomography assessment of aromatic L-amino acid decarboxylase gene transfer in Parkinson's disease. (2010)
  • Author(s): Asari, S.
  • Organizer: The 16^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 栃木県総合文化センター(宇都宮)
  • Year and Date: 2010-07-03
• [Presentation] Liver-restricted expression of the canine factor VIII gene facilitates prevention of inhibitor formation in factor VIII-deficient mice. (2010)
  • Author(s): Mimuro, J.
  • Organizer: The 16^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 栃木県総合文化センター(宇都宮)
  • Year and Date: 2010-07-03
• [Presentation] Successful factor IX expression by IV administration of AAV8 vectors in macaques. (2010)
  • Author(s): Mizukami, H.
  • Organizer: The 16^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 栃木県総合文化センター(宇都宮)
  • Year and Date: 2010-07-03
• [Presentation] Mesenchymal stem cells as a cellular vehicle for cancer-targeted gene therapy (2010)
  • Author(s): Uchibori R
  • Organizer: The 16th Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 栃木県総合文化センター(宇都宮)
  • Year and Date: 2010-07-02
• [Presentation] Mesenchymal stem cells as a cellular vehicle for cancer-targeted gene therapy. (2010)
  • Author(s): Uchibori, R.
  • Organizer: The 16^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 栃木県総合文化センター(宇都宮)
  • Year and Date: 2010-07-02
• [Presentation] Editing cis elements required for AAV integration to enhance AAVS1-targeted integration (2010)
  • Author(s): Urabe M
  • Organizer: The 16th Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 栃木県総合文化センター(宇都宮)
  • Year and Date: 2010-07-01
• [Presentation] The effect of DMXAA(56-dimethylxanthenone-4-acetic acid) on MSC accumulation in tumor sites (2010)
  • Author(s): Uchibori R
  • Organizer: The 16th Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 栃木県総合文化センター(宇都宮)
  • Year and Date: 2010-07-01
• [Presentation] Development of an enzyme-linked immunosorbent assay method for rapid screening of anti-AAV-2 antibodies. (2010)
  • Author(s): Ito, T.
  • Organizer: The 16^<th> Annual Meeting of the Japan Societyof Gene Therapy
  • Place of Presentation: 栃木県総合文化センター(宇都宮)
  • Year and Date: 2010-07-01
• [Presentation] Choice of small-sized promoter for AAV-mediated factor IX expression in skeletal muscle. (2010)
  • Author(s): Kume, A.
  • Organizer: The 16^<th> Amual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 栃木県総合文化センター(宇都宮)
  • Year and Date: 2010-07-01
• [Presentation] AAV vector-mediated expression of interleukin-10 suppresses proteinuria in Zucker fatty rats through the improvement of glomerular hypertrophy and podocyte injuy. (2010)
  • Author(s): Ogura, M.
  • Organizer: The 16^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 栃木県総合文化センター(宇都宮)
  • Year and Date: 2010-07-01
• [Presentation] Prevalence of neutralizing antibodies against adeno-associated virus 2, 8 and 9 in non-human primate colonies using sensitive assay system (2010)
  • Author(s): Yagi, H.
  • Organizer: The 16^<th> Amual Meeting of the Japan Societyof Gene Therapy
  • Place of Presentation: 栃木県総合文化センター(宇都宮)
  • Year and Date: 2010-07-01
• [Presentation] Editing cis elements required for AAV integration to enhance AAVS1-targeted integration (2010)
  • Author(s): Urabe, M.
  • Organizer: The 16^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 栃木県総合文化センター(宇都宮)
  • Year and Date: 2010-07-01
• [Presentation] The effect of DMXAA (56-dimethylxanthenone-4-acetic acid) on MSC accumulation in tumor sites. (2010)
  • Author(s): Uchibori, R.
  • Organizer: The 16^<th> Annual Meeting of the Japan Societyof Gene Therapy
  • Place of Presentation: 栃木県総合文化センター(宇都宮)
  • Year and Date: 2010-07-01
• [Presentation] Promoter selection for AAV vector-mediated factor IX expression in skeletal muscles. (2010)
  • Author(s): Kume, A.
  • Organizer: The 13^<th> Annual Meeting of the American Society of Gene Therapy
  • Place of Presentation: Marriott Wardman Park Hotel (Washington, D.C.)
  • Year and Date: 2010-05-21
• [Presentation] Robust and sustained factor IX expression by IV administration of AAV8-based vectors in macaques. (2010)
  • Author(s): Mizukami, H.
  • Organizer: The 13^<th> Annual Meeting of the American Society of Gene Therapy
  • Place of Presentation: Marriott Wardman Park Hotel (Washington, D.C.)
  • Year and Date: 2010-05-21
• [Presentation] Systemic delivery of interleukin-10 by an AAV vector attenuates obesity-related glomerulopathy in Zucker fatty rats. (2010)
  • Author(s): Ogura, M.
  • Organizer: The 13^<th> Annual Meeting of the American Society of Gene Therapy
  • Place of Presentation: Marriott Wardman Park Hotel (Washington, D.C.)
  • Year and Date: 2010-05-21
• [Presentation] Prevalence of neutralizing antibodies against adeno-associated virus 2, 8 and 9 in non-human primate colonies. (2010)
  • Author(s): Yagi, H.
  • Organizer: The 13^<th> Annual Meeting of the American Society of Gene Therapy
  • Place of Presentation: Marriott Wardman Park Hotel (Washington, D.C.)
  • Year and Date: 2010-05-21
• [Presentation] NF-κB activity regulates MSC accumulation at tumor sites (2010)
  • Author(s): Uchibori R
  • Organizer: The 13th Annual Meeting of the American Society of Gene Therapy
  • Place of Presentation: Marriott Wardman Park Hotel(Washington, D. C.)
  • Year and Date: 2010-05-20
• [Presentation] NF-κB activity regulates MSC accumulation at tumor sites. (2010)
  • Author(s): Uchibori, R.
  • Organizer: The 13^<th>Annual Meeting of the American Society of Gene Therapy
  • Place of Presentation: Marriott Wardman Park Hotel (Washington, D.C.)
  • Year and Date: 2010-05-20
• [Presentation] AAV-vector mediated expression of interleukin-10 in skeletal muscle suppresses progressive renal disease in a rat model of metabolic syndrome. (2009)
  • Author(s): Ogura, M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-12-09
• [Presentation] Mesenchymal stem cells(MSCs) as a platform for cancer gene therapy (2009)
  • Author(s): Uchibori R
  • Organizer: 第71回日本血液学会学術集会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2009-10-23
• [Presentation] Mesenchymal stem cells(MSCs)as a platform for cancer gene therapy (2009)
  • Author(s): Uchibori, R.
  • Organizer: 第71回日本血液学会学術集会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2009-10-23
• [Presentation] VCAM-1 expression following TNF-alpha stimulation is required, for tumor tropism of mesenchymal stem cells (2009)
  • Author(s): Uchibori R
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-10-03
• [Presentation] VCAM-1 expression following TNF-alpha stimulation is required for tumor tropism of mesenchymal stem cells (2009)
  • Author(s): Uchibori, R.
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-10-03
• [Presentation] Cancer-targeted gene therapy using MSCs as a platform (2009)
  • Author(s): Uchibori R
  • Organizer: Annual Meeting of the International Society for Cell and Gene Therapy of Cancer
  • Place of Presentation: Ireland
  • Year and Date: 2009-09-03
• [Presentation] Cancer-targeted gene therapy using MSCs as a platform. (2009)
  • Author(s): Uchibori, R.
  • Organizer: Annual Meeting of the International Society for Cell and Gene Therapy of Cancer
  • Place of Presentation: Ireland
  • Year and Date: 2009-09-03
• [Presentation] Mechanism of MSC(Mesenchymal stem/stromal cell) accumulation at tumor sites (2009)
  • Author(s): Uchibori R
  • Organizer: The 15th Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 大阪大学コンベンションセンター(大阪)
  • Year and Date: 2009-07-11
• [Presentation] Neutralizing antibody against vector capsid affects liver-mediated factor IX expression in non-human primates using AAV vectors. (2009)
  • Author(s): Mizukami, H.
  • Organizer: The 15^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 大阪大学コンベンションセンター(大阪)
  • Year and Date: 2009-07-11
• [Presentation] Phase I trial of AAV vector-mediated gene delivery of aromatic L-amino acid decarboxylase for Parkinson's disease. (2009)
  • Author(s): Muramatsu, S.
  • Organizer: The 15^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 大阪大学コンベンションセンター(大阪)
  • Year and Date: 2009-07-11
• [Presentation] Liver-targeted gene therapy with a self-complementary AAV ameliorated brain aminergic deficit in phenylketonuria mice. (2009)
  • Author(s): Yagi, H.
  • Organizer: The 15^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 大阪大学コンベンションセンター(大阪)
  • Year and Date: 2009-07-11
• [Presentation] Mechanism of MSC(Mesenchymal stem/stromal cell)accumulation at tumor sites. (2009)
  • Author(s): Uchibori, R.
  • Organizer: The 15^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 大阪大学コンベンションセンター(大阪)
  • Year and Date: 2009-07-11
• [Presentation] Systemic delivery of interleukin-10 by an AAV vector suppressed progressive renal disease in an animal model of metabolic syndrome. (2009)
  • Author(s): Ogura, M.
  • Organizer: The 15^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 大阪大学コンベンションセンター(大阪)
  • Year and Date: 2009-07-10
• [Presentation] shRNA-mediated gene silencing in non-human primates with AAV8. (2009)
  • Author(s): Kubodera, T.
  • Organizer: The 15^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 大阪大学コンベンションセンター(大阪)
  • Year and Date: 2009-07-09
• [Presentation] A sensitive assay for neutralizing antibody against AAV serotypes in cluding 8 and 9. (2009)
  • Author(s): Mizukami, H.
  • Organizer: The 15^<th> Annual Meeting of the Japan Society of Gene Therapy
  • Place of Presentation: 大阪大学コンベンションセンター(大阪)
  • Year and Date: 2009-07-09
• [Presentation] Neutralizing antibody affects liver-mediated factor IX- expression in non-human primates using AAV vectors. (2009)
  • Author(s): Mizukami, H.
  • Organizer: The 12^<th> Annual Meeting of the American Society of Gene Therapy
  • Place of Presentation: San Diego Convention Center(San Diego)
  • Year and Date: 2009-05-30
• [Presentation] Aromatic L-amino acid decarboxylase gene therapy for Parkinson's disease : results from an open-label, phase I trial. (2009)
  • Author(s): Muramatsu, S
  • Organizer: The 12^<th> Annual Meeting of the American Society of Gene Therapy
  • Place of Presentation: San Diego Convention Center(San Diego)
  • Year and Date: 2009-05-29
• [Presentation] Roles of inflammatory cytokines in tumor tropism of mesenchimal stromal cells (2009)
  • Author(s): Uchibori R
  • Organizer: The 12th Annual Meeting of the American Society of Gene Therapy
  • Place of Presentation: San Diego Convention Center(San Diego)
  • Year and Date: 2009-05-28
• [Presentation] Roles of inflammatory cytokines in tumor tropism of mesenchimal stromal cells. (2009)
  • Author(s): Uchibori, R.
  • Organizer: The 12^<th> Annual Meeting of the American Society of Gene Therapy
  • Place of Presentation: San Diego Convention Center(San Diego)
  • Year and Date: 2009-05-28
• [Presentation] Rloe of TNF-α in the MSC accumulation at tumor sites. (2009)
  • Author(s): Uchibori, R.
  • Organizer: The 7^<th> Stem Cell Research Symposium
  • Place of Presentation: Izumi Garden Gallery(Tokyo)
  • Year and Date: 2009-05-15
• [Presentation] Mechanism of MSC(mesenchymal stem/stromal cell) accumulation at the sites of tumors (2009)
  • Author(s): Uchibori R
  • Organizer: The 14th Congress of the European Hematology Association
  • Place of Presentation: Germany
• [Presentation] Mechanism of MSC(mesenchymal stem/stromal cell)accumulation at the sites of tumors. (2009)
  • Author(s): Uchibori R
  • Organizer: The 14^<th> Congress of the European Hematology Association
  • Place of Presentation: Germany