| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Research Abstract |
The cultured CD34-positive cells of juvenile myelomonocytic leukemia(JMML) patient were transfected the Yamanaka 4 genes, and cultured on mouse embryonic fibroblasts. Two-3 weeks later, many cell clusters with the RAS mutation were formed and negative for CD34. These cells were compatible with induced pluripotent(iPS) stem cells. We succeeded the establishment of iPS cells with myeloid differentiation potencial from the cultured JMML CD34-positive cells. We are going to elucidate pathogenesis of JMML and properties of the leukemic stem cells.
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