Cellular signaling system in Psoriatic epidermis

• Project/Area Number: 21390323
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Asahikawa Medical College
• Principal Investigator: IIZUKA Hajime 旭川医科大学, 医学部, 教授 (90113513)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Akemi 旭川医科大学, 医学部, 准教授 (30241441) ; TAKAHASHI Hiswtoshi 旭川医科大学, 医学部, 講師 (00216748) ; HONMA Masaru 旭川医科大学, 医学部, 講師 (50344578)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
• Keywords: 乾癬 / 表皮細胞 / 細胞内情報伝達 / ポドプラニン / IL-22 / 乾癬表皮細胞 / MAP kinase / STAT3 / Raf / podoplanin / corneodesmosin / corneodesosin

Research Abstract

Podoplanin expression of keratinocytes was induced by IL-22 in a STAT-3-dependent manner. The podoplanin expression was highly upregulated in basal cells of granular layer-negative psoriatic epidermis compared with that of granular layer positive less hyperproliferative epidermis. Furthermore, podoplanin expression was more marked on basal cells of psoriatic rete ridges where stem cell-derived transit amplifying cells are assumed to be located. The finite proliferative potential of transit amplifying cells was confirmed in keratinocyte following podoplanin induction. These results indicate that podoplanin might be a novel marker of transit amplifying cells of epidermis.

Research Products

• [Journal Article] Podoplanin expression is inversely correlated with granular layer/filaggrin formation in psoriatic epidermis. (2013)
  • Author(s): Honma M, Fujii M, Iinuma S, Minami-Hori M, Takahashi H, Ishida-Yamamoto A., Iizuka H
  • Journal Title: J Dermatol Volume: 40 Pages: 296-7
• [Journal Article] Podoplanin expression in wound and hyperproliferative psoriatic epidermis: Regulation by TGF-beta and STAT-3 activating cytokines (2012)
  • Author(s): Honma M, Minami-Hori M, Takahashi H, Iizuka H
  • Journal Title: J Dermatol Sci Volume: 65 Pages: 134-140
• [Journal Article] Lamellar granule secretion starts before the estqablishment of tight junction barrier for paracellular tracers in mammalian epidermis (2012)
  • Author(s): Ishida-Yamamoto A, Kishibe M, Murakami M, Honma M, Takahashi H, Iizuka H
  • Journal Title: PloS One Volume: 7 Pages: 1-7
• [Journal Article] Kallikrein-Related Peptidase 8.Dependent Skin Wound Healing Is Associated with Upregulation of Kallikrein-Related Peptidase 6 and PAR2 (2012)
  • Author(s): Kishibe M, Bando Y, Tanaka T, Ishida-Yamamoto A, Iizuka H, Yoshida S
  • Journal Title: J Invest Dermatol Volume: 132 Pages: 1717-24
• [Journal Article] Over-expression of kallikrein related peptidases in palmoplantar pustulosis. (2012)
  • Author(s): Kaneko T
  • Journal Title: J Dermatol Sci Volume: 67 Pages: 73-76
  • Peer Reviewed
• [Journal Article] Podoplanin expression inwound and hyperproliferative psoriatic epidermis : Eegulation by TGF-β and STAT-3 activating cytokines (2012)
  • Author(s): Honma, Minami-Hori, Takahashi, Iizuka
  • Journal Title: Journal of Dermatological Science Volume: 65 Pages: 134-140
  • Peer Reviewed
• [Journal Article] Tight junction-related multilayered network structures in mammalian epidermis. (2012)
  • Author(s): Ishida-Yamamoto A, Kishibe M, Murakami M, Honma M, Takahashi H, Iizuka H.
  • Journal Title: PLoS ONE Volume: 7 Issue: 2 Pages: 1-7
  • DOI: 10.1371/journal.pone.0031641
  • Peer Reviewed
• [Journal Article] Kallikrein-related peptidase 8-dependent skin wound healing is associated with upregulation of kallikrein-related peptidase 6 and PAR2 (2012)
  • Author(s): Kishibe M, Bando Y, Tanaka T, Ishida-Yamamoto A, Iizuka H, Yoshida S
  • Journal Title: J Invest Dermatol Volume: (印刷中) Issue: 6 Pages: 1717-1724
  • DOI: 10.1038/jid.2012.18
  • Peer Reviewed
• [Journal Article] Analysis of psoriasis patients registered in Japanese Society for Psoriasis Research from 2002 to 2008 (2011)
  • Author(s): Takahashi H, Nakamura K, Kaneko F, Nakagawa H, Iizuka H
  • Journal Title: J Dermatol Volume: 38 Pages: 1125-9
• [Journal Article] Management of patients with psoriasis in Japan (2011)
  • Author(s): Iizuka H
  • Journal Title: Adv Psorisasis & Inflammatory skin diseases Pages: 126-132
• [Journal Article] Effects of serine palmitoyltransferase inhibitor ISP-1 on the stratum corneum of intact mouse skin (2011)
  • Author(s): Mizukoshi K, Matsumoto K, Hirose R, Fujita T, Ishida-Yamamoto A, Iizuka H
  • Journal Title: Biol Pharm Bull Volume: 34 Pages: 1383-9
• [Journal Article] Podoplanin expression in wound and hyperproliferative psoriatic epidermis : regulation by TGF-beta and STAT-3 activating cytokines, IFN-eanma, IL-6, and IL22 (2011)
  • Author(s): Honma, Minami-Hori, Iizuka, et al
  • Journal Title: Journal of Investigative Der,atology Volume: (投稿中)
  • Peer Reviewed
• [Journal Article] Serum cytokines and growth factor levels in Japanese patients with psoriasis (2010)
  • Author(s): Takahashi H, Tsuji H, Hashimoto Y, Ishida-Yamamoto A, Iizuka H
  • Journal Title: Clin Exp Dermatol Volume: 35 Pages: 645-9
• [Journal Article] Tight junctions in the stratum corneum explain spacial differences in corneodesmosome degradation (2010)
  • Author(s): Igawa S, Kishibe M, Murakami M Honma M, Takahashi H, Iizuka H, Ishida-Yamamoto A
  • Journal Title: Exp Dermatol Volume: 20 Pages: 53-57
• [Journal Article] Adiponectin and leptin modulate cell proliferation and cytokine secretion of normal human keratinocytes and T lymphocytes (2010)
  • Author(s): Takahashi H, Honma M, Ishida-Yamamoto A, Iizuka H
  • Journal Title: J Dermatol Sci Volume: 59 Pages: 143-145
  • NAID: 10027402055
• [Journal Article] Tight junctions in the stratum corneum explain spacial differences in corneodesmosome degradation. (2010)
  • Author(s): Igawa, Kisibe, Ishida-Yamamoto, Iizuka, et al
  • Journal Title: Experimental Dermatology Volume: 20 Pages: 53-57
  • Peer Reviewed
• [Journal Article] Serum cytokines and growth factor levels in Japanese patients with psoriasis (2010)
  • Author(s): Takahashi, Iizuka, et al
  • Journal Title: Clinical and Experimental Dermatology Volume: 35 Pages: 645-649
  • Peer Reviewed
• [Journal Article] Prevalence of metabolic syndrome in Japanese psoriasis patients. (2010)
  • Author(s): Takahashi, Honma, Iizuka, et al
  • Journal Title: Journal of Dermatolical Science 57 Pages: 143-144
  • NAID: 10027399756
  • Peer Reviewed
• [Journal Article] Acrosyringium is the main site of the vesicle/pustule formation in palm oplantar pustulosis. (2010)
  • Author(s): Murakami, Ishida-Yamamoto, Iizuka, et al
  • Journal Title: Journal of Investigative Dermatology (In press, on line publication)
  • NAID: 120004606781
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Hajime Iizuka
  • Journal Title: Therapy of Skin Diseases 3.1 Psoriasis(Springer) Pages: 193-206
• [Journal Article] Prevalence of obesityadiposity in Japanese psoriasis patients ; adiposity is correlated with the severity of psoriasis. (2009)
  • Author(s): Takahashi, Iizuka, et al
  • Journal Title: Journal of Dermatological Science 54 Pages: 61-63
  • Peer Reviewed
• [Presentation] Podoplanin alters beta1-integrin-mediated cell adhesion and initiates terminal differentiation in human epidermal keratinocytes (2012)
  • Author(s): Honma M, Fujii M, Takahashi H, Iizuka H
  • Organizer: The 37^<th> annual meetiThe 37th annual meeting of the Japanese Society for Investigative Dermatology
• [Presentation] A desquamation paradox of atopic dermatitis: markedly remaining corneodesmosomes despite the increased serine protease activity in vitro (2012)
  • Author(s): Igawa S, Iimuma S, Kishibe M, Minami-Hori M, Honma M, Murakami M, Ishida-Yamamoto A, Iizuka H
  • Organizer: The 37^<th> annual meeting of the Japanese Society for Investigative Dermatology
• [Presentation] Cell density-dependent induction of podoplanin through EGFR-STAT3 signaling pathway contributes to increased cell invasion of SCC cells (2012)
  • Author(s): Fujii M, Honma M, Takahashi H, Ishida-Yamamoto A, Iizuka H
  • Organizer: The 37^<th> annual meeting of the Japanese Society for Investigative Dermatology
• [Presentation] 乾癬の治療：生物学製剤の時代におけるピラミッド計画 (2012)
  • Author(s): 飯塚 一
  • Organizer: 第２７回日本乾癬学会学術大会
  • Place of Presentation: 新潟市 朱鷺メッセ
  • Invited
• [Presentation] 乾癬表皮における組織構築の変動と角化異常の再評価 (2011)
  • Author(s): 飯塚一
  • Organizer: 第26回日本乾癬学会学術大会
  • Place of Presentation: 大阪国際会議場(特別(招待)講演)
  • Year and Date: 2011-09-09
• [Presentation] Kallikrein-related peptidase 8 is involved in skin wound healing the rough upregulation of KLK6 and protease activated receptor 2 (2011)
  • Author(s): Kishibe M, Igawa S, Ishida-Yamamoto A, Iizuka H
  • Organizer: The 36^<th> annual meeting of the Japanese Society for Investigative Dermatology
• [Presentation] Epidermal lamellar granule secretion starts before establishment of paracellular permeability barrier (2011)
  • Author(s): Ishida-Yamamoto A, Kishibe M, Murakami M, Iizuka H
  • Organizer: The 36^<th> annual meeting of the Japanese Society for Investigative Dermatology
• [Presentation] Podoplanin induces upregulated cell mortility through activation of coffilin and matrix metalloproteinase-9 in HaCat cells (2011)
  • Author(s): Fujii M, Honma M, Takahashi H, Ishida-Yamamoto A, Iizuka H
  • Organizer: The 36^<th> annual meeting of the Japanese Society for Investigative Dermatology
• [Presentation] A new era of the pathophysiology of psoriasis: anti-TNF-alfa agents as a smart bomb with multiple targets (2010)
  • Author(s): Iizuka H
  • Organizer: The 35^<th> annual meeting of the Japanese Society for Investigative Dermatology
• [Presentation] Tight junctions in the stratum crneum explain special differences in corneodesmosome degradation (2010)
  • Author(s): Ishida-Yamamoto A, Igawa S, Kishibe M, Murakami M, Honma M, Iizuka H
  • Organizer: The 35th annual meeting of the Japanese Society for Investigative Dermatology
• [Presentation] Podoplanin upregulates cell mortility through the activation of cofilin and matrix metalloproteinase in immortalized epidermal keratinocytes (2010)
  • Author(s): Honma M, Fujii M, Iizuka H
  • Organizer: The 35^<th> annual meeting of the Japanese Society for Investigative Dermatology
• [Presentation] Adiponectin and leptin modulte cell proliferation and cytokine expressions of normal human keratinocytes and T lymphocytes (2010)
  • Author(s): Takahashi H, Tsuji H, Iizuka H
  • Organizer: The 35^<th> annual meeting of the Japanese Society for Investigative Dermatology
• [Presentation] TIP-DC-Th17細胞学説からみた乾癬の病態と治療 (2009)
  • Author(s): 飯塚一
  • Organizer: 第60回日本皮膚科学会中部支部学術大会:特別講演
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-10-10