Analysis of regulation of inflammation on the skin : Toward development of platform for new anti-inflammatory drug

• Project/Area Number: 21390324
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Chiba University
• Principal Investigator: MATSUE Hiroyuki 千葉大学, 大学院・医学研究院, 教授 (10250424)
• Co-Investigator(Kenkyū-buntansha): YAO Jian 山梨大学, 医学工学総合研究部, 准教授 (50303128) ; KAMADA Noriaki 千葉大学, 医学部附属病院, 講師 (00334186) ; KAMBE Naotomo 千葉大学, 大学院・医学研究院, 准教授 (50335254)
• Co-Investigator(Renkei-kenkyūsha): KAMADA Noriaki 千葉大学, 医学部附属病院, 講師 (00334186) ; KAMBE Naotomo 千葉大学, 大学院・医学研究院, 准教授 (50335254)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2009: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 内科 / 免疫学 / 生体分子 / 薬剤反応性 / 臨床 / 樹状細胞 / ギャップ結合 / 免疫抑制 / 抗炎症剤 / 接触皮膚炎

Research Abstract

Our research revealed a new role of Gap junction(formed by connexins)-mediated cell-cell communication, which is involved in drug-mediated cell injury. Furthermore, we found that hemichannel (also formed by connexins) mediated heavy metal-induced cell injury. Finally, our data suggested that skin inflammation is also mediated in part by Gap junction-mediated signal transductions, and further studies are required to definitely demonstrate such signaling pathways in skin inflammation.

Research Products

• [Journal Article] Kerion celsi due to Arthroderma incurvatum infection in a Sri Lankan child: species identification and analysis of area-dependent genetic polymorphism (2012)
  • Author(s): Iwasawa M. T., Togawa Y, Akita F., Kambe N., Matsue H., Yaguchi T, Nishimura K
  • Journal Title: Medical Mycology Volume: 39 Issue: 7 Pages: 205-206
  • DOI: 10.3109/13693786.2012.671968
  • Peer Reviewed
• [Journal Article] Maximum growth temperature test for cutaneous mycobacterium chelonae predicts the efficacy of thermal therapy (2012)
  • Author(s): Ota R., Iwasawa M. T., Ohkusu K. , Kambe N., Matsue H
  • Journal Title: J Dermatol Volume: 39 Issue: 2 Pages: 205-206
  • DOI: 10.1111/j.1346-8138.2011.01246.x
  • NAID: 10031184871
  • Peer Reviewed
• [Journal Article] Reciprocal regulation between proinflammatory cytokine-induced iNOS and connexin43 in bladder smooth muscle cells (2011)
  • Author(s): Li K, Yao J, Shi L, Sawada N, Chi Y, Yan Q, Fukayama M, Zakohji H, Araki I, Kitamura Mand Takeda M
  • Journal Title: J Biol Chem Volume: 286 Issue: 48 Pages: 41552-41562
  • DOI: 10.1074/jbc.m111.274449
  • Peer Reviewed
• [Journal Article] Connexin43 hemichannels contribute to cadmium- induced oxidative stress and cell injury (2011)
  • Author(s): Fang X, Huang T, ZhuY, Jiang JX, Wang P, Kitamura Mand Yao J
  • Journal Title: Antioxid Redox Signal Volume: 14 Issue: 12 Pages: 2427-2439
  • DOI: 10.1089/ars.2010.3150
  • Peer Reviewed
• [Journal Article] Nonsteroidal anti-inflammatory drug flufenamic acid is a potent activator of AMPK (2011)
  • Author(s): Chi Y, Li K, Yan Q, Koizumi S, Shi L, Takahashi S, Zhu Y, Matsue H, Takeda M, Kitamura M, Yao J
  • Journal Title: J Pharmacol Exp Ther Volume: 339 Issue: 1 Pages: 257-266
  • DOI: 10.1124/jpet.111.183020
  • Peer Reviewed
• [Journal Article] Follicular keratosis and bullous formation are typical signs of extragenital lichen sclerosus (2011)
  • Author(s): Kimura A., Kambe N. , Satoh T., Togawa Y., Suehiro K., Matsue, H
  • Journal Title: J Dermatol Volume: 38 Issue: 8 Pages: 834-836
  • DOI: 10.1111/j.1346-8138.2010.01079.x
  • NAID: 10031147105
  • Peer Reviewed
• [Journal Article] Autoinflammatory diseases and the inflammasome : mechanisms of IL-1 beta activation leading to neutrophil-rich skin disorders (2011)
  • Author(s): Kambe N, Satoh T, Nakamura Y, Iwasawa M, Matsue H
  • Journal Title: Inflammation and Regeneration Volume: 31-1 Pages: 72-80
• [Journal Article] Lymphocutaneous Type of Nocardiosis Caused by Nocardia vinacea in a Patient with Polymyositis (2011)
  • Author(s): Iwasawa M. T., Togawa Y., Kamada N., Kambe N., Matsue H., Yazawa K., Yaguchi T., Mikami Y
  • Journal Title: Mycopathologia Volume: 172 Issue: 1 Pages: 47-53
  • DOI: 10.1007/s11046-011-9391-0
  • Peer Reviewed
• [Journal Article] Disruption of gap junction attenuates aminoglycoside- elicited renal tubular cell injury (2010)
  • Author(s): Yao J, Huang T, Fang X, Chi Y, Zhu Y, Wan Y, Matsue H, and Kitamura M
  • Journal Title: Br J Pharmacol Volume: 160 Issue: 8 Pages: 2055-2068
  • DOI: 10.1111/j.1476-5381.2010.00860.x
  • Peer Reviewed
• [Journal Article] Melanoma in association with acquired melanocytic nevus in Japan: A review of cases in the literature (2010)
  • Author(s): Togawa Y., Nakamura, Y., Kamada, N., Kambe, N., Takahashi, Y., Matsue, H
  • Journal Title: Int J Dermatol Volume: 49 Issue: 12 Pages: 1362-1367
  • DOI: 10.1111/j.1365-4632.2010.04602.x
  • Peer Reviewed
• [Journal Article] Toxic epidermal necrolysis with severe hyperbilirubinemia : Complete re-epithelialization after bilirubin reduction therapies (2010)
  • Author(s): Kamada N., Yoneyama K., Togawa Y., Suehiro K., Shinkai, H., Yokota, M., Matsuda K., Oda, S., Hirasawa, H., Matsue, H
  • Journal Title: J Dermatol Volume: 37 Pages: 534-536
  • NAID: 10026546593
  • Peer Reviewed
• [Journal Article] 皮膚の免疫学-肥満細胞と好塩基球 (2010)
  • Author(s): 松江弘之
  • Journal Title: 日本皮膚科学会雑誌 Volume: 120 Issue: 6 Pages: 2171-4
  • DOI: 10.1111/j.1346-8138.2009.00770.x
  • NAID: 130004708704
• [Journal Article] Mast cells mediate neutrophil recruitment and vascular leakage through the NLRP3 inflammasome in histamine-independent urticaria (2009)
  • Author(s): Nakamura, Y., Kambe, N., Saito, M., Nishikomori, R., Kim, Y. G., Murakami, M., Nunez, G. & Matsue, H.
  • Journal Title: J Exp Med Volume: 206 Issue: 5 Pages: 1037-1046
  • DOI: 10.1084/jem.20082179
  • Peer Reviewed
• [Journal Article] Murine fetal skin-derived cultured mast cells : A useful tool for discovering novel functions of skin mast cells (2009)
  • Author(s): Matsue H, Kambe N, Shimada S
  • Journal Title: J Invest Dermatol Volume: 129 Issue: 5 Pages: 1120-1125
  • DOI: 10.1038/jid.2009.44
  • Peer Reviewed
• [Journal Article] Mast cells mediate neutrophil recruitment and vascular leakage through the NLRP3 inflammasome in histamine-independent urticaria. (2009)
  • Author(s): Nakamura Y
  • Journal Title: J Exp Med. 206 Pages: 1037-1046
  • Peer Reviewed
• [Journal Article] Murine fetal skin-derived cultured mast cells : A useful tool for discovering functions of skin mast cells. (2009)
  • Author(s): Matsue H
  • Journal Title: J Invest Dermatol. 129 Pages: 1120-1125
  • Peer Reviewed
• [Presentation] Oligomerization of NLRP3 is induced dwonstream of cathepsin B in NLRP3-induced rapid programmed cell death (2011)
  • Author(s): Satoh T, Kambe N, Matsue H
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉市
• [Presentation] Homopolymerization of ASC using FKBP12 chimeric protein induced rapid cell death accompanied with IL-1 beta processing (2011)
  • Author(s): Satoh T, Kambe N, Matsue H
  • Organizer: 41st Annual ESDR Meeting
  • Place of Presentation: Barcelona, Spain
• [Presentation] Peripheral CD14+ monocytes from early-onset sarcoidosis associated with NOD2 mutation do not show the molecular signature of NF-kappa B signaling (2011)
  • Author(s): Kambe N, Satoh T, Matsue H
  • Organizer: 41st Annual ESDR Meeting
  • Place of Presentation: Barcelona, Spain
• [Presentation] Enhanced NF-kappaB activation with NLRP3 activator correlates with disease activities in cryopyin-associated periodic syndrome (2010)
  • Author(s): Satoh T, Kambe N, Matsue H
  • Organizer: 40th Annual ESDR Meeting
  • Place of Presentation: Helsinki, Finland
• [Presentation] Enhanced NF-kappaB activation after the stimulation with NLRP3 activator correlates with disease activities in cryopyin-associated periodic syndrome (2010)
  • Author(s): Satoh T, Kambe N, Saito M, Nishikomori R, Matsue H
  • Organizer: Autoinflammation
  • Place of Presentation: Amsterdam, The Netherlands
• [Book] 肥満細胞、好塩基球 (2010)
  • Author(s): 松江弘之, 大塚篤司
  • Publisher: 中外医学社