| Project/Area Number |
21390336
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
門前 暁 弘前大学, 大学院・保健学研究科, 助教 (20514136)
吉野 浩教 弘前大学, 大学院・保健学研究科, 助教 (10583734)
高橋 賢次 弘前大学, 大学院・保健学研究科, 助教 (00400143)
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| Co-Investigator(Renkei-kenkyūsha) |
ABE Yoshinao 弘前大学, 大学院・医学研究科, 教授 (10167950)
KONDO Takashi 富山大学, 医学部, 教授 (40143937)
TAKAHASHI Kenji 鳥取大学, 農学部・獣医学科, 准教授 (00400143)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Keywords | 造血幹細胞 / 放射線 / 遺伝子 / 個体差 |
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| Research Abstract |
The purpose of this study was to clarify the individual radiosensitivity of human hematopoietic stem/progenitor cells and the relation between the characters of cell surface antigens, proliferative potentials and gene expressions, thus identifying what is the factor (s) to regulate the individual radiosensitivity of human hematopoietic stem cells. The following points were revealed.
1. It suggest that the radiosensitivity of individual hematopoietic stem/progenitor cells (HSPCs) is related to the number of progenitor cells in the CD34^+ cells especially dependent on the presence of immature HSPCs such as Tie-2^+ cells.
2. The mRNA expression of Nrf2 target genes such as HO-1, FTH1, NQO1, GSR and TXNRD1 was significantly increased after 2 Gy X irradiation. A statistically significant negative correlation was observed between the surviving fraction of HSPCs and the intrinsic NQO1 mRNA expression, indicating that HSPCs in which NQO1 mRNA levels are low may also be radioresistant. The present results suggest that the antioxidant system associated with Nrf2 is involved in the radiosensitivity of HSPCs.
3. A significant correlation was observed between the surviving fraction of hematopoietic progenitor cells obtained after 0.5 Gy irradiation and the age of the blood donors. In particular, this correlation was more evident in males, with a correlation coefficient of-0.318 (P=0.043). A correlation between the individual responsiveness of hematopoietic progenitor cells to ionizing irradiation, particularly to a low-dose of irradiation (such as 0.5 Gy), and aging has been established.
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