| Project/Area Number |
21390379
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Yamaguchi University |
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Principal Investigator |
OKA Masaaki 山口大学, 大学院・医学系研究科, 教授 (70144946)
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| Co-Investigator(Kenkyū-buntansha) |
IIZUKA Norio 山口大学, 医学部附属病院, 准教授 (80332807)
HAMAMOTO Yoshihiko 山口大学, 大学院・医学系研究科, 教授 (90198820)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | 肝癌 / メチル化遺伝子 / セルフリーDNA / メチル化 |
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| Research Abstract |
There are few blood tests for an efficient detection of hepatocellular carcinoma(HCC) associated with hepatitis C virus(HCV) infection. The abilities of quantitative analyses of 7 genes hypermethylation in serum DNA, -fetoprotein(AFP) and prothrombin-induced vitamin K absence II(PIVKA-II), and various combinations to detect HCC were evaluated. An optimal hybrid detector, built using data for 2 methylated genes(SPINT2 and SRD5A2), AFP, and PIVKA-II, achieved the most satisfactory ability to detect HCC in the training cohort(n=164) and an independent validation cohort(n=258). In the validation cohort, accuracy, sensitivity, and specificity of the hybrid detector for detection of HCC were 81. 4%, 73. 2%, and 87. 7%, respectively. Notably, even when detecting HCC 2cm in diameter, the hybrid detector maintained markedly high abilities(84. 6% accuracy, 72. 2% sensitivity, 87. 7% specificity). These results show that the optimal hybrid blood detector can detect HCV-related HCC more accurately.
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