Human Umbilical Cord Blood-derived Mononuclear Cell Transplantation Improves Pulmonary Arterial Hypertension in Immunodeficiency Mouse
| Project/Area Number |
21390392
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
KITAGWA Tetsuya 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (80240886)
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| Co-Investigator(Kenkyū-buntansha) |
KITAICHI Takashi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (20335813)
NAKAGAWA Ryuji 徳島大学, 病院, 助教 (10403685)
MAEDA Kazutoshi 徳島大学, 病院, 准教授 (30294680)
SUGANO Mikio 徳島大学, 病院, 特任助教 (70563807)
KANBARA Tamotsu 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (20380082)
KUROBE Hirotsugu 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (30380083)
YOSHIDA Homare 徳島大学, 病院, 助教 (50448338)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | 臍帯血 / 細胞移植 / 未熟肺血管床 |
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| Research Abstract |
Backgrounds and Objectives. The purpose of this study is to investigate whether human umbilical cord blood-derived mononuclear cell(hUCB-MNC) transplantation improves the development of pulmonary arterial hypertension(PAH) in monocrotaline(MCT)-treated mice. HUCB-MNC transplantation may have a new therapeutic potential for several congenital heart defects with primitive pulmonary vasculatures. Methods. MCT-induced PAH model was made using BALB/ c Slc-nu immunodeficiency mouse. HUCB-MNCs were adjusted to 1 x 107/ 0. 25 mL of concentration, and injected intravenously into those mice 4 weeks after MCT administration. The mice were divided into five groups :(1) vehicle-injected mice ;(2) MCT-treated mice ;(3) hUCB-MNC transplantation for PAH mice ;(4) hUCB-MNC+ plasma transplantation for PAH mice ;(5) plasma transplantation for PAH mice. Assessment of PAH were performed by the ratio of right ventricular to ventricular septum plus left ventricular weight(RV/ S+ LV), medial thickness of small pulmonary arteries, and pulmonary acceleration time/ ejection time(PAT/ ET) of the main pulmonary artery by echocardiography. Results. RV/ S+ LV ratio, medial thickness of small pulmonary arteries and PAT/ ET were significantly improved by hUCB-MNC transplantation with or without plasma, respectively. Conclusions. Our data shows that hUCB-MNC transplantation improves MCT-induced PAH and its efficacy is dependent on hUCB-MNCs themselves
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Autologous Peripheral Blood-Derived Mononuclear Cells Autologous Peripheral Blood-Derived Mononuclear Cells Induced by Erythropoietin Improve Critical Ischemic Limbs2012
Author(s)
Kanbara T, Kurobe T, Kitaichi T, Sugano M, Nakayama T, Kinoshita H, Iwase T, Akaike M, Abe M, Sata M, Matsumoto T, Kitagawa T
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Journal Title
Annals of Vascular Diseases
Volume: 5(1)
Pages: 52-60
Related Report
Peer Reviewed
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[Journal Article] Human cord-blood cell transplantation to monocrotaline-induced pulmonary arterial hypertension in mice2011
Author(s)
Sugano M, Kurobe H, Hirata Y, Nakagawa R, Yoshida H, Kitaichi T, Nakayama T, Kinoshita H, Motoki T, Kanbara T, Sugasawa N, Miki M, Maeda K, Sata M, Kitagawa T
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Journal Title
Gen Thorac Cardiovasc Surg
Volume: 59
Pages: 213-213
Related Report
Peer Reviewed
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[Journal Article] Coronary atherosclerosis is associated with macrophage polarization in epicardial adipose tissue2011
Author(s)
Hirata Y, Tabata M, KurobeH, Motoki T, Akaike M, Nishio C, Higashida M, Mikasa H, Nakaya Y, Takanashi S, Igarashi T, Kitagawa T, Sata M
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Journal Title
J Am Coll Cardiol
Volume: 58(3)
Pages: 248-55
Related Report
Peer Reviewed
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[Journal Article] Ezetimibe monotherapy ameliorates vascular function in patients with hypercholesterolemia through decreasing oxidative stress2011
Author(s)
Kurobe H, Aihara K, Higashida M, Hirata Y, Nishiya M, Matsuoka Y, Kanbara T, Nakayama T, Kinoshita H, Sugano M, Fujimoto E, Kurobe A, Sugasawa N, Kitaichi T, Akaike M, Sata M, Matsumoto T, Kitagawa T
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Journal Title
J Atheroscler Thromb
Volume: 18(12)
Pages: 1080-9
NAID
Related Report
Peer Reviewed
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[Journal Article] Coronary atherosclerosis is associated with macrophage polarization in epicardial adipose tissue2011
Author(s)
Y. Hirata, M. Tabata, H. Kurobe, T. Motoki, M. Akaike, C. Nishio, M. Higashida, H. Mikasa, Y. Nakaya, S. Takanashi, T. Igarashi, T. Kitagawa, M. Sata
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Journal Title
J. Am. Coll. Cardiol.
Volume: 58
Issue: 3
Pages: 248-255
DOI
Related Report
Peer Reviewed
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[Journal Article] Undiminished regulatory T cells in the thymus of patients with myasthenia gravis2010
Author(s)
Matsui N, Nakane S, Saito F, Ohigashi I, Nakagawa Y, Kurobe H, Takizawa H, Mitsui T, Kondo K, Kitagawa T, Takahama Y, Kaji R
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Journal Title
Neurology
Volume: 74(10)
Pages: 816-20
Related Report
Peer Reviewed
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[Journal Article] Role of Hif-1αin T cells as a negative regulator in development of vascular remodeling2010
Author(s)
Kurobe H, Urata M, Ueno M, Ono S, Izawa-Ishizawa Y, Fukuhara Y, Gukuhara Y, Yu L, Ripen AM, Kanbara T, Aihara K, Ishizawa K, Akaike M, Gonzalez FJ, Tamaki T, Takahama Y, Yoshizumi M, Kitagawa T, Tomita S
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Journal Title
Arteriosclerosis, Thrombosis, and Vascular Biology
Volume: 30(2)
Pages: 210-7
Related Report
Peer Reviewed
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