Tyrosine kinase gene mutation and chemotherapy sensitivity in lung cancers.

• Project/Area Number: 21390394
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Nagoya City University
• Principal Investigator: FUJII Yoshitaka 名古屋市立大学, 大学院・医学研究科, 教授 (40156831)
• Co-Investigator(Kenkyū-buntansha): SASAKI Hidefumi 名古屋市立大学, 大学院・医学研究科, 准教授 (00336695) ; YANO Motoki 名古屋市立大学, 大学院・医学研究科, 准教授 (40315883)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
• Keywords: 肺癌 / ERCC1 / NRF2 / KIF5B/RET / RET阻害剤 / FISH / MRP3 / EGFR / KIF5B / RET / 遺伝子転座 / FGFR1 / コピー数 / Kras / AKT2 / MEK1 / NFE2L2 / 扁平上皮癌 / βチュブリン / MCL1 / BCL2L1

Research Abstract

ERCC1 C8092A and codon118C/T gene polymorphism statuses were evaluated for lung cancer patients who recieved adjuvant chemotherapy. We have investigated NRF2 gene mutation statuses in lung squamous cell carcinomas and compared with copy number, MRP3(multi-drug resistance-associated protein 3) expression and other clinico-pathological backgrounds. Novel KIF5B/RET translocation was found in lung cancer using a next-generation sequencing assay. KIF5B/RET transfected cells were sensitive to anti-RET drugs. We have developed fluorescence in situ hybridization(FISH) assay to detect KIF5B/RET transfusion gene.

Research Products

• [Journal Article] Identification of new ALK and RET gene fusions from colorectal and lung cancer biopsies (2012)
  • Author(s): Lipson D, Capelletti M, Yelensky R, Sasaki H(24番目), et al
  • Journal Title: Nat Med Volume: 18 Pages: 382-384
  • Peer Reviewed
• [Journal Article] Increased FGFR1 copy number in lung squamous cell carcinomas (2012)
  • Author(s): Sasaki H, Shitara M, Yokota K, Hikosaka Y, Moriyama S, Yano M, Fujii Y
  • Journal Title: Mol Med Report Volume: 5 Pages: 725-728
  • Peer Reviewed
• [Journal Article] Thoracic and cardiovascular surgery in Japan during 2009 : Annual report by The Japanese Association for Thoracic Surgery (2011)
  • Author(s): Sakata R, Fujii Y, Kuwano H
  • Journal Title: Gen Thorac Cardiovasc Surg Volume: 59 Pages: 636-667
  • NAID: 10030549420
• [Journal Article] Prognostic factors of pathologic stage 1B non-small cell lung cancer (2011)
  • Author(s): Yano M, Sasaki H, Moriyama S, Kawano O, Hikosaka Y, Fujii Y
  • Journal Title: Ann Thorac Cardiovasc Surg Volume: 17 Pages: 58-62
  • Peer Reviewed
• [Journal Article] LKB1 gene alterations in surgically resectable adenocarcinoma of the lung (2011)
  • Author(s): Okuda K, Sasaki H, Hikosaka Y, Kawano O, Moriyama S, Yano M, Fujii Y
  • Journal Title: Surg Today Volume: 41 Pages: 107-110
  • NAID: 10027818817
  • Peer Reviewed
• [Journal Article] Excision repair cross complementation group 1 polymorphisms predict overall survival after platinum-based chemotherapy for completely resected non-small cell lung cancer (2011)
  • Author(s): Okuda K, Sasaki H, Hikosaka Y, Kawano O, Yukiue H, Yano M, Fujii Y
  • Journal Title: J Surg Res Volume: 168 Pages: 206-212
  • Peer Reviewed
• [Journal Article] Prognostic factors of pathologic stage 1B non-small cell lung cancer (2011)
  • Author(s): Yano M, et al
  • Journal Title: Interact Cardiovasc Thorac Surg Volume: 17 Pages: 58-62
  • Peer Reviewed
• [Journal Article] Evaluation of Kras gene mutation and copy number gain in non-small cell lung cancer. (2011)
  • Author(s): Sasaki H, et al.
  • Journal Title: J Thorac Oncol Volume: 5(10) Pages: 15-20
  • Peer Reviewed
• [Journal Article] Thoracic and cardiovascular surgery in Japan during 2008 : Annual report by The Japanese Association for Thoracic Surgery (2010)
  • Author(s): Sakata R, Fujii Y, Kuwano H
  • Journal Title: Gen Thorac Cardiovasc Surg Volume: 58 Pages: 356-383
  • NAID: 10026514287
• [Journal Article] NFE2L2 gene mutation in male Japanese squamous cell carcinoma of the lung (2010)
  • Author(s): Sasaki H, Hikosaka Y, Okuda K, Kawano O, Moriyama S, Yano M, Fujii Y
  • Journal Title: J Thorac Oncol Volume: 5 Pages: 786-789
  • Peer Reviewed
• [Journal Article] MEK1 and AKT2 mutations in Japanese lung cancer (2010)
  • Author(s): Sasaki H, Hikosaka Y, Kawano O, Moriyama S, Yano M, Fujii Y
  • Journal Title: J Thorac Oncol Volume: 5 Pages: 597-600
  • Peer Reviewed
• [Journal Article] The landscape of somatic copy-number alteration across human cancers (2010)
  • Author(s): Beroukhim R, Mermel CH, Porter D, Sasaki H(33番目), et al
  • Journal Title: Nature Volume: 463 Pages: 899-905
  • Peer Reviewed
• [Journal Article] MEK1 and AKT2 mutations in Japanese lung cancer (2010)
  • Author(s): Sasaki H, et al.
  • Journal Title: J Thorac Oncol Volume: 5(5) Pages: 597-600
  • Peer Reviewed
• [Journal Article] NFE2L2 gene mutation in male Japanese squamous cell carcinoma of the lung. (2010)
  • Author(s): Sasaki H, et al.
  • Journal Title: J Thorac Oncol Volume: 5(6) Pages: 786-789
  • Peer Reviewed
• [Journal Article] The landscape of somatic copy-number alteration across human cancers (2010)
  • Author(s): Beroukhim R, Sasaki H(33番目), et al.
  • Journal Title: Nature 463 Pages: 899-905
  • Peer Reviewed
• [Journal Article] Thoracic and cardiovascular surgery in Japan during 2007 (2009)
  • Author(s): Ueda Y, Fujii Y, Kuwano H
  • Journal Title: Gen Thorac Cardiovasc Surg Volume: 57 Pages: 488-513
• [Journal Article] Epidermal growth factor receptor gene amplification in surgical resected Japanese lung cancer (2009)
  • Author(s): Sasaki H, Shimizu S, Okuda K, Kawano O, Yukiue H, Yano M, Fujii Y
  • Journal Title: Lung Cancer Volume: 64 Pages: 295-300
  • Peer Reviewed
• [Journal Article] Epidermal growth factor receptor gene amplification in surgical resected non-small cell lung cancer (2009)
  • Author(s): Sasaki H, et al.
  • Journal Title: Lung Cancer 64(3) Pages: 295-300
  • Peer Reviewed
• [Journal Article] Thymoma with dissemination : efficacy of microscopic total resection of disseminated nodules (2009)
  • Author(s): Yano M, Sasaki H, et al.
  • Journal Title: World J Surg 33(7) Pages: 1425-1431
  • Peer Reviewed
• [Presentation] 非小細胞肺癌におけるKras遺伝子変異とコピー数増加に関する検討 (2011)
  • Author(s): 佐々木秀文
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-05
• [Presentation] <ワークショップ>:胸腺上皮性腫瘍の治療up to data胸腺癌におけるTS, OPRT, ERCC1, Class IIIβ-tubulinタンパク発現の検討 (2011)
  • Author(s): 奥田勝裕、横田圭右、矢野智紀、佐々木秀文、森山悟、彦坂雄、設楽将之、藤井義敬
  • Organizer: 第52回日本肺癌学会総会
  • Place of Presentation: 大阪
• [Presentation] ERCC1 polymorphisms predict overall survival after platinum-based chemotherapy for complemently resected NSCLC (2011)
  • Author(s): Okuda K, Sasaki H, Yano M, Moriyama S, Hikosaka Y, Fujii Y
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] 肺癌におけるNRF2遺伝子変異は男性扁平上皮癌に多く予後と相関する (2010)
  • Author(s): 佐々木秀文
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2010-09-22
• [Presentation] 本邦における肺癌登録の現状と展望-肺癌登録合同委員会について (2010)
  • Author(s): 藤井義敬
  • Organizer: 第25回日本肺癌学会ワークショップ
  • Place of Presentation: 東京
  • Year and Date: 2010-07-03
• [Presentation] 日本人肺扁平上皮癌におけるNRF2遺伝子変異は予後と相関する (2010)
  • Author(s): 佐々木秀文、彦坂雄、森山悟、矢野智紀、藤井義敬
  • Organizer: 第63回日本胸部外科学会定期学術集会
  • Place of Presentation: 大阪
• [Presentation] 肺癌におけるNRF2遺伝子変異は男性扁平上皮癌に多く予後と相関する (2010)
  • Author(s): 佐々木秀文、奥田勝裕、彦坂雄、森山悟、矢野智紀、藤井義敬
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] NFE2L2 gene mutation in Japanese lung cancer patients (2010)
  • Author(s): Sasaki H, Hikosaka Y, Kawano O, Okuda K, Moriyama S, Yano M, Fujii Y
  • Organizer: AACR 101^<th> annual meeting
  • Place of Presentation: Washington DC
• [Presentation] Epidermal growth factor receptor gene amplification in surgical resected Japanese lung cancer (2009)
  • Author(s): Hidefumi Sasaki, et al.
  • Organizer: 13th world conference on lung cancer(世界肺癌学会)
  • Place of Presentation: サンフランシスコ(米国)
  • Year and Date: 2009-08-01
• [Presentation] 日本人肺腺癌におけるMEK1およびAKT2遺伝子変異の検討 (2009)
  • Author(s): 佐々木秀文、奥田勝裕、雪上晴弘、矢野智紀、藤井義敬
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 呼吸器外科手術の診療ガイドライン:縦隔腫瘍取り扱い規約とガイドライン (2009)
  • Author(s): 藤井義敬
  • Organizer: 第26回日本呼吸器外科学会総会
  • Place of Presentation: 北九州
• [Book] 「呼吸器外科学」改訂第4版 (2009)
  • Author(s): 正岡昭、藤井義敬、矢野智紀、佐々木秀文、原真咲、羽田園城、平手博之、祖父江和哉、河野匡、伊達洋至、岡部和倫、植村貞繁、丹羽宏、正岡昭、藤井義敬
  • Publisher: 南山堂
• [Patent(Industrial Property Rights)] KIF5B遺伝子とRET遺伝子との間の転座を検出するFISHアッセイ (2012)
  • Inventor(s): 佐々木秀文
  • Industrial Property Rights Holder: 名古屋市立大学、ダコ・ジャパン株式会社、株式会社GSP研究所
  • Industrial Property Number: 2012-015939
  • Filing Date: 2012-01-27