Development of bone regeneration methods for large bone defect using bone marrow derived mesenchymal stem cells.
| Project/Area Number |
21390418
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SOTOME Shinichi 東京医科歯科大学, 医歯学総合研究科, 寄付講座教員 (20401391)
ENOMOTO Mitsuhiro 東京医科歯科大学, 医歯学総合研究科, 寄付講座教員 (90451971)
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| Co-Investigator(Renkei-kenkyūsha) |
AE Keisuke 東京医科歯科大学, 医学部, 非常勤講師 (20376726)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | 骨再生 / 骨髄間葉系幹細胞 / 分化能 / 骨形成能 / 大型骨欠損 / 分化抑制因子 / ephrin-EPHA / 骨癒合能 / 骨髄間葉系細胞 / 継代培養 / 細胞増殖 / マイクロアレイ |
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| Research Abstract |
Bone marrow mesenchymal stem cells(MSCs) have pluripotent differentiation capability and are expected to be used for regenerative medicine. However, the differentiation capability decreases with their prolonged proliferation. Moreover, our previous studies showed that the such cells had not only less bone formation capability but also suppressed bone formation. In this study, we focused on EPHA5 as the suppressor candidate from microarray and co-culture study.
We achieved 5 cm long bone regeneration using MSCs in a monkey femur bone defect model. The regenerated bone proved its bone union capability that is one of the impossible characteristics of natural bone.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Hybrid Grafting using Bone Marrow Aspirate combined with Porous beta-Tricalcium Phosphate and Trephine Bone for Lumbar Posterolateral Spinal Fusion : A Prospective, Comparative Study-Versus Local Bone Grafting-2012
Author(s)
Yamada T, Yoshii T, Sotome S, Yuasa M, Kato T, Arai Y, Kawabata S, Tomizawa S, Sakaki K, Hirai T, Shinomiya K, Okawa A
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Journal Title
Spine
Volume: (In press)
Issue: 3
Pages: 174-179
DOI
Related Report
Peer Reviewed
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[Journal Article] Repair of large osteochondral defects in rabbits using porous hydroxyapatite/collagen(HAp/Col) and fibroblast growth factor-2(FGF-2)2010
Author(s)
Maehara H, Sotome S, Yoshii T, Torigoe I, Kawasaki Y, Sugata Y, Yuasa M, Hirano M, Mochizuki N, Kikuchi M, Shinomiya K, Okawa A
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Journal Title
Journal of Orthopaedic Research
Volume: 28(5)
Pages: 677-686
Related Report
Peer Reviewed
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[Journal Article] Effects of gamma-ray irradiation on mechanical properties, osteoconductivity, and absorption of porous hydroxyapatite/collagen2010
Author(s)
Kawasaki Y, Sotome S, Yoshii T, Torigoe I, Maehara H, Sugata Y, Hirano M, Mochizuki N, Shinomiya K, Okawa A
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Journal Title
Journal of Biomedical and Materials Research B Applied Biomaterials
Volume: 92(1)
Pages: 161-167
Related Report
Peer Reviewed
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[Journal Article] Bone regeneration with autologous plasma, bone marrow stromal cells, and porous beta-tricalcium phosphate in nonhuman primates2009
Author(s)
Torigoe I, Sotome S, Tsuchiya A, Yoshii T, Maehara H, Sugata Y, Ichinose S, Shinomiya K, Okawa A
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Journal Title
Tissue Engineering Part A
Volume: 15(7)
Pages: 1489-1499
Related Report
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[Journal Article] Hybrid Grafting using Bone Marrow Aspirate combined with Porous beta-Tricalcium Phosphate and Trephine Bone for Lumbar Posterolateral Spinal Fusion : A Prospective, Comparative Study-Versus Local Bone Grafting
Author(s)
Yamada T, Yoshii T, Sotome S, Yuasa M, Kato T, Arai Y, Kawabata S, Tomizawa S, Sakaki K, Hirai T, Shinomiya K, Okawa A
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Journal Title
Related Report
Peer Reviewed
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