Establishment of mouse model of synovial sarcomas using lineage-specific gene targeting system
| Project/Area Number |
21390420
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Gen 京都大学, 再生医科学研究所, 准教授 (40243258)
KATO Tomohisa 京都大学, 再生医科学研究所, 講師 (50301247)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2009: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
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| Keywords | 滑膜肉腫 / 神経堤 / 融合遺伝子 / ノックインマウス |
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| Research Abstract |
To prove out hypothesis that cell-of-origin of synovial sarcomas(SS) is neural crest derived cells, we established transgenic mice in which the expression of SS-specific fusion gene, SYT-SSX was induced in neural crest cells using the P0 gene as a driver. The expression of fusion gene in utero inhibited the development of craniofacial bone and cartilage tissues causing stillbirth. Then we established inducible transgenic mice using tamoxifen-ER system. Treatment with tamoxifen successfully induced the expression of SYT-SSX and also its downstream gene in P0 positive cells, indicating that this mouse model is a useful tool to understand the development of synovial sarcomas.
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] Prostaglandin E2 receptor type 2 receptor-selective agonist prevents the degeneration of articular cartilage in rabbit knees with traumatic instability2011
Author(s)
Mitsui H, Aoyama T, Furu M, Ito K, Jin Y, Maruyama T, Kanaji T, Fujimura S, Sugihara H, Nishiura A, Otsuka T, Nakamura T, Toguchida J
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Journal Title
Arthritis Res Ther
Volume: 13(5)
Pages: 146-146
URL
Related Report
Peer Reviewed
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[Journal Article] Identification of AFAP1L1 as a prognostic marker for spindle cell sarcomas2011
Author(s)
Furu M, Kajita Y, Nagayama S, Ishibe T, Shima Y, Nishijo K, Uejima D, Takahashi R, Aoyama T, Nakayama T, Nakamura T, Nakashima Y, Ikegawa M, Imoto S, Katagiri T, Nakamura Y, Toguchida J
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Journal Title
Oncogene
Volume: 30(38)
Issue: 38
Pages: 4015-25
DOI
Related Report
Peer Reviewed
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[Journal Article] Involvement of cancer biomarker C7orf24 in the growth of human osteosarcoma2011
Author(s)
Uejima D, Nishijo K, Kajita Y, Ishibe T, Aoyama T, Kageyama, Iwaki H, Nakamura T, Iida, Yoshiki T, Toguchida J
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Journal Title
Anticancer Res
Volume: 31(4)
Pages: 1297-305
URL
Related Report
Peer Reviewed
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[Journal Article] Gene Expression Profiling of Synovial Sarcoma : Distinct Signature of Poorly Differentiated Type2010
Author(s)
Nakayama R, Mitani S, Nakagawa T, Hasegawa T, Kawai A, Morioka H, Yabe H, Toyama Y, Ogose A, Nakayama, T, Toguchida, J, Yoshida T, Ichikawa H
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Journal Title
Am J Surg Pathol
Volume: 34(11)
Pages: 1599-607
URL
Related Report
Peer Reviewed
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[Journal Article] His tone modifiers, YY1 and p300, regulate the expression of cartilage-specific gene, chondromodulin-I, in mesenchymal stem cells2010
Author(s)
Aoyama T, Okamoto T, Fukiage K, Otsuka S, Furu M, Ito K, Jin Y, Ueda M, Nagayama S, Nakayama T, Nakamura T, Toguchida J
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Journal Title
J Biol Chem
Volume: 285(39)
Issue: 39
Pages: 29842-50
DOI
Related Report
Peer Reviewed
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