The functional analysis of the nucleotide-sugar transporter SLC35D1 and SLC35D2 in cartilage using knockout mice model.
| Project/Area Number |
21390428
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kobe Pharmaceutical University (2012-2013)
National Research Institute for Child Health and Development (2009-2011) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ASAHARA Hiroshi 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (70294460)
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| Project Period (FY) |
2009-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Keywords | 糖ヌクレオチド輸送体 / 軟骨疾患 / コンドロイチン硫酸 / 骨軟骨異形成症 / 関節軟骨 / 糖鎖 / 生体分子 / 関節疾患 |
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| Research Abstract |
The chondroitin sulfate (CS) is required for various cartilage functions. The nucleotide-sugar transporter SLC35D1 and SLC35D2 supply substrates to polymerize CS. I analyzed compound mutant mice harboring in SLC35D1 KO and hypomorphic, and SLC35D2 KO mutations. As a result of investigations, I clarified that both transporters play the essential role for CS synthesis of proteoglycans in cartilage, (2) various combinations of mutant alleles of both coding genes induce to step wise reduction of CS producing level in cartilage, (3) the skeletal abnormalities are observed in response to the reduction level of CS synthesis. I also investigated SLC35D1 function using Flox mutant of SLC35d1 mice. I observed critical loss of intestinal functions in adult mice lacking SLC35D1 by inducible gene disruption strategy. The nobel function of SLC35D1 in intestine is useful to survey unidentified leaky-type mutations of SLC35D1 in human, which may survive during perinatal period.
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Report
(5 results)
Research Products
(20 results)
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[Journal Article] CCN3 protein participates in bone regeneration as an inhibitory factor.2013
Author(s)
Matsushita Y, Sakamoto K, Tamamura Y, Shibata Y, Minamizato T, Kihara T, Ito M, Katsube K, Hiraoka S, Koseki H, Harada K, Yamaguchi A.
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Journal Title
The Journal of Biological Chemistry
Volume: 288
Issue: 27
Pages: 19973-19985
DOI
Related Report
Peer Reviewed
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