| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Research Abstract |
The chondroitin sulfate (CS) is required for various cartilage functions. The nucleotide-sugar transporter SLC35D1 and SLC35D2 supply substrates to polymerize CS. I analyzed compound mutant mice harboring in SLC35D1 KO and hypomorphic, and SLC35D2 KO mutations. As a result of investigations, I clarified that both transporters play the essential role for CS synthesis of proteoglycans in cartilage, (2) various combinations of mutant alleles of both coding genes induce to step wise reduction of CS producing level in cartilage, (3) the skeletal abnormalities are observed in response to the reduction level of CS synthesis. I also investigated SLC35D1 function using Flox mutant of SLC35d1 mice. I observed critical loss of intestinal functions in adult mice lacking SLC35D1 by inducible gene disruption strategy. The nobel function of SLC35D1 in intestine is useful to survey unidentified leaky-type mutations of SLC35D1 in human, which may survive during perinatal period.
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