A study of male infertility as genome diseases-Recombination of genome DNA and sperm typing-

• Project/Area Number: 21390438
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kanazawa University
• Principal Investigator: KOH Eitetsu 金沢大学, 医学系, 准教授 (90283134)
• Co-Investigator(Kenkyū-buntansha): NAMIKI Mikio 金沢大学, 医学系, 教授 (70155985) ; MAEDA Yuji 金沢大学, 附属病院, 助教 (20377394) ; KANAYA Jiro 金沢大学, 附属病院, 助教 (80432128)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 精子 / ヒトゲノム / ハプロタイプ,再組換え / 繰り返し配列 / Y染色体 / 減数 / ハプロタイプ / FISH / 再組換え / 減数分裂

Research Abstract

We evaluated an analysis of the single sperm DNA in the meiotic system regarding process of spermatogenesis. According to the sequence of the haplotype, much repeat sequence existed in Y chromosome. We confirmed much these repeat sequence involved a human endogenous retrovirus(HERV). The phylogenetic analysis showed a Y-specific palindromic structural existed since old world monkey. This study proved the presence of palindrome 1, 2 since an early evolutionary stage was associated with spermatogenesis

Research Products

• [Journal Article] Human postmeiotic sex chromatin and its impact on sex chromosome evolution (2012)
  • Author(s): Sin HS, Ichijima Y, Koh E, Namiki M, Namekawa SH.
  • Journal Title: Genome Res Volume: 22 Issue: 5 Pages: 827-36
  • DOI: 10.1101/gr.135046.111
  • Peer Reviewed
• [Journal Article] A novel Y chromosome microdeletion with the loss of an endogenous retrovirus(ERV)-related testis-specific transcript in AZFb region (2011)
  • Author(s): Sin HS, Koh E, Taya M, IIjima M, Sugimoto K, Maeda Y, Yoshida A, Iwamoto T, Namiki M
  • Journal Title: J Urol Volume: 186 Issue: 4 Pages: 1545-52
  • DOI: 10.1016/j.juro.2011.05.044
  • Peer Reviewed
• [Journal Article] A novel Y chromosome microdeletion with the loss of an endo genous retrovirus(ERV)-related testis-specific transcript in AZFb region. (2011)
  • Author(s): Sin HS, Koh E, 他
  • Journal Title: J Urol. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Human endogenous retrovirus K14C drove genomic diversification of the Y chromosome during primate evolution (2010)
  • Author(s): Sin HS, Koh E, Kim DS, Murayama M, Sugimoto K, Maeda Y, Yoshida A, Namiki M
  • Journal Title: J Hum Genet Volume: 55 Pages: 717-25
  • NAID: 10030737445
  • Peer Reviewed
• [Journal Article] Features of constitutive gr/gr deletion in a Japanese population (2010)
  • Author(s): Sin HS, Koh E, Shigehara K, Sugimoto K, Maeda Y, Yoshida A, Kyono K, Namiki M
  • Journal Title: Hum Reprod Volume: 25 Issue: 9 Pages: 2396-403
  • DOI: 10.1093/humrep/deq191
  • Peer Reviewed
• [Journal Article] 精子関連遺伝子 (2009)
  • Author(s): 高栄哲, 並木幹夫
  • Journal Title: ホルモンと臨床 57 Pages: 59-65
• [Presentation] HERV-K14C contibutees to male speciation and spermatogenesis during primate evolution (2011)
  • Author(s): Sin, H., Koh E., Shin M., Shigehara K., Sugimoto K., Maeda Y., Namiki, M
  • Organizer: 11th International symposium on spermatology
  • Place of Presentation: Okinawa Convention Center(沖縄県)
  • Year and Date: 2011-06-29
• [Presentation] ESTIMATION OF THE EVOLUTIONARY TIMING (2011)
  • Author(s): Eitetsu Koh, Ho-Su Sin, Kazuyoshi Shigehara, Kazuhiro Sugimoto, Yuji Maeda, and Mikio Namiki
  • Organizer: ASA 36th annual conference
  • Place of Presentation: Montreal Hyatt Regency(Canada)
  • Year and Date: 2011-04-03
• [Presentation] ゲノム病としての男性不妊症-AZF(Azoospermia factor)精子形成領域の微小欠失機構とヒト内因性レトロウイルスからみたY染色体ゲノム分化 (2010)
  • Author(s): 高栄哲、並木幹夫
  • Organizer: 日本遺伝学会第82回大会
  • Place of Presentation: 北海道大学高等教育機能開発総合センター(北海道)
  • Year and Date: 2010-09-20
• [Presentation] ゲノム病としての男性不妊症 (2010)
  • Author(s): Ho-Su Sin, 高栄哲, 他
  • Organizer: 日本遺伝学会第82回大会
  • Place of Presentation: 北海道大学高等教育機能開発総合センター(北海道)
  • Year and Date: 2010-09-20
• [Presentation] Genomic rearrangement and azoospermia (2009)
  • Author(s): 高栄哲
  • Organizer: 9^<th> International Congress of Andrology
  • Place of Presentation: Barcelona(Spain)convention center
  • Year and Date: 2009-03-08