Basic and developmental research of a novel anti-tumor protein with dendritic cell-inducible activity.

• Project/Area Number: 21390441
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Okayama University
• Principal Investigator: NASU Yasutomo 岡山大学, 岡山大学病院, 教授 (20237572)
• Co-Investigator(Kenkyū-buntansha): KUMON Hiromi 岡山大学, 大学院・医歯薬学総合研究科, 教授 (30144760) ; KAKU Haruki 岡山大学, 岡山大学病院, 講師 (60346426) ; 雑賀 隆史 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (10314676) ; 枝村 康平 岡山大学, 大学病院, 医員 (90535816)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000); Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: REIC/Dkk-3 / 前立腺癌 / 腎細胞癌 / 抗癌免疫 / 樹状細胞 / REIK/DKK-3 / サイトカイン / REIC / 遺伝子治療 / 蛋白治療. / ノックアウトマウス

Research Abstract

The REIC/Dkk-3 gene has been reported to be a tumor suppressor and the expression is significantly downregulated in a broad range of cancer cell types. In this study, we focused the REIC protein and clarified the immunological anti-cancer function and the possible utility for the clinical use. We believe that current findings are available to create innovative cancer-targeting and immunological therapy for the cancer diseases including prostate cancer.

Research Products

• [Journal Article] Preclinical Safety and Efficacy of in Situ REIC/Dkk-3 Gene Therapy for Prostate Cancer. (2012)
  • Author(s): Kawauchi K, Watanabe M, Kaku H, Huang P, Sasaki K, Sakaguchi M, Ochiai K, Huh NH, Nasu Y, Kumon H.
  • Journal Title: Acta Med Okayama Volume: 66(1) Pages: 7-16
  • NAID: 120003850603
  • Peer Reviewed
• [Journal Article] Laparoscopic management of complicated urachal remnants in adults (2012)
  • Author(s): Araki M, Saika T, Araki D, Kobayashi Y, Uehara S, Watanabe T, Yamada K, Nasu Y, Kumon H
  • Journal Title: World J Urol.
  • Peer Reviewed
• [Journal Article] Partial sensitization of human bladder cancer cells to a gene-therapeutic adenovirus carrying REIC/Dkk-3 by downregulation of BRPK/PINK1. (2012)
  • Author(s): Jin Y, Murata H, Sakaguchi M, Kataoka K, Watanabe M, Nasu Y, Kumon H
  • Journal Title: Huh NH. Oncol Rep. Volume: 27(3) Pages: 695-9
  • DOI: 10.3892/or.2011.1543
  • Peer Reviewed
• [Journal Article] Partial sensitization of human bladder cancer cells to a gene-therapeutic adenovirus carrying REIC/Dkk-3 by downregulation of BRPK/PINK1 (2012)
  • Author(s): Jin Y, Murata H, et al
  • Journal Title: Oncol Rep Volume: 27 Pages: 695-9
  • Peer Reviewed
• [Journal Article] Could salvage surgery after chemotherapy have clinical impact on cancer survival of patients with metastatic urothelial carcinoma? (2011)
  • Author(s): Bekku K, Saika T, Kobayashi Y, Kioshimoto R, Kanbara T, Nasu Y, Kumon H.
  • Journal Title: Int J Clin Oncol.
  • NAID: 10031176243
  • Peer Reviewed
• [Journal Article] Single nucleotide polymorphism WRN Leu1074Phe is associated with prostate cancer susceptibility in Chinese subjects. (2011)
  • Author(s): Wang L, Kaku H, Huang P, Xu K, Yang K, Zhang J, Li M, Xie L, Wang X, Sakai A, Watanabe M, Nasu Y, Shimizu K, Kumon H, Na Y.
  • Journal Title: Acta Med Okayama Volume: 65(5) Pages: 315-23
  • Peer Reviewed
• [Journal Article] T2-weighted image/T2-weighted image fusion in postimplant dosimetry of prostate brachytherapy. (2011)
  • Author(s): Katayama N, Takemoto M, Yoshio K, Katsui K, Uesugi T, Nasu Y, Matsushita T, Kaji M, Kumon H, Kanazawa S
  • Journal Title: J Radiat Res Volume: 52(5) Pages: 680-4
  • NAID: 10029456187
  • Peer Reviewed
• [Journal Article] Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1. (2011)
  • Author(s): Ochiai K, Watanabe M, Ueki H, Huang P, Fujii Y, Nasu Y, Noguchi H, Hirata T, Sakaguchi M, Huh NH, Kashiwakura Y, Kaku H, Kumon H
  • Journal Title: Biochem Biophys Res Commun Volume: 412(2) Pages: 391-5
  • Peer Reviewed
• [Journal Article] Preoperative positive urine cytology is a risk factor for subsequent development of bladder cancer after nephroureterectomy in patients with upper urinary tract urothelial carcinoma. (2011)
  • Author(s): Kobayashi Y, Saika T, Miyaji Y, Saegusa M, Arata R, Akebi N, Takenaka T, Manabe D, Nasu Y, Kumon H.
  • Journal Title: World J Urol.
  • Peer Reviewed
• [Journal Article] Advanced two-step transcriptional amplification as a novel method for cancer-specific gene expression and imaging. (2011)
  • Author(s): Watanabe M, Ueki H, Ochiai K, Huang P, Kobayashi Y, Nasu Y, Sasaki K, Kaku H, Kashiwakura Y, Kumon H
  • Journal Title: Oncol Rep. Volume: 26(4) Pages: 769-75
  • DOI: 10.3892/or.2011.1371
  • Peer Reviewed
• [Journal Article] Implications of transcriptional factor, OCT-4, in human bladder malignancy and tumor recurrence. (2011)
  • Author(s): Huang P, Chen J, Wang L, Na Y, Kaku H, Ueki H, Sasaki K, Yamaguchi K, Zhang K, Saika T, Nasu Y, Watanabe M, Kumon H
  • Journal Title: Med Oncol.
  • Peer Reviewed
• [Journal Article] Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1 (2011)
  • Author(s): Ochiai K, Watanabe M, et al
  • Journal Title: Biochem Biophys Res Commun Volume: 412 Pages: 391-5
  • Peer Reviewed
• [Journal Article] Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development. (2010)
  • Author(s): Zhang K, Watanabe M, Kashiwakura Y, Li SA, Edamura K, Huang P, Yamaguchi K, Nasu Y, Kobayashi Y, Sakaguchi M, Ochiai K, Yamada H, Takei K, Ueki H, Huh NH, Li M, Kaku H, Na Y, Kumon H.
  • Journal Title: Int J Oncol Volume: 37(6) Pages: 1495-501
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor activator inhibitors(HAI-1 and HAI-2) are potential targets in uterine leiomyosarcoma. (2010)
  • Author(s): Nakamura K, Abarzua F, Hongo A, Kodama J, Nasu Y, Kumon H, Hiramatsu Y.
  • Journal Title: Int J Oncol Volume: 37(3) Pages: 605-14
  • Peer Reviewed
• [Journal Article] Potent antitumor effects of combined therapy with a telomerase-specific, replication-competent adenovirus(OBP-301) and IL-2 in a mouse model of renal cell carcinoma. (2010)
  • Author(s): Huang P, Kaku H, Chen J, Kashiwakura Y, Saika T, Nasu Y, Urata Y, Fujiwara T, Watanabe M, Kumon H.
  • Journal Title: Cancer Gene Ther. Volume: 17(7) Pages: 484-91
  • Peer Reviewed
• [Journal Article] A randomized phase II trial of personalized peptide vaccine plus low dose estramustine phosphate(EMP) versus standard dose EMP in patients with castration resistant prostate cancer. (2010)
  • Author(s): Noguchi M, Kakuma T, Uemura H, Nasu Y, Kumon H, Hirao Y, Moriya F, Suekane S, Matsuoka K, Komatsu N, Shichijo S, Yamada A, Itoh K.
  • Journal Title: Cancer Immunol Immunother. Volume: 59(7) Pages: 1001-9
  • Peer Reviewed
• [Journal Article] Down-regulation of BiP/GRP78 sensitizes resistant prostate cancer cells to gene-therapeutic overexpression of REIC/Dkk-3. (2010)
  • Author(s): Tanimoto R, Sakaguchi M, Abarzua F, Kataoka K, Kurose K, Murata H, Nasu Y, Kumon H, Huh NH
  • Journal Title: Int J Cancer Volume: 126(7) Pages: 1562-9
  • NAID: 120004724432
  • Peer Reviewed
• [Journal Article] Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development (2010)
  • Author(s): Zhang K, et al
  • Journal Title: Int J Oncol Volume: 37 Pages: 1495-1501
  • Peer Reviewed
• [Journal Article] 5αDH-DOC (5α-dihydro-deoxycorticosterone) activates androgen receptor in castration-resistant prostate cancer (2010)
  • Author(s): Uemura M, et al
  • Journal Title: Cancer Sci Volume: 101 Pages: 1897-1904
  • Peer Reviewed
• [Journal Article] Potent antitumor effects of combined therapy with a telomerase-specific, replication-competent adenovirus (OBP-301) and IL-2 in a mouse model of renal cell carcinoma (2010)
  • Author(s): Huang P, et al
  • Journal Title: Cancer Gene Ther Volume: 17 Pages: 484-491
  • Peer Reviewed
• [Journal Article] A randomized phase II traial of personalized peptide vaccine plus low dose estramustin phosphate (EMP) versus standard dose EMP in patients with castration resistant prostate cancer (2010)
  • Author(s): Noguchi M, et al
  • Journal Title: Cancer Immunol Immunother Volume: 59 Pages: 1001-1009
  • Peer Reviewed
• [Journal Article] REIC/Dkk-3 stable transfection reduces the malignant phenotype of mouse prostate cancer RM9 cells (2009)
  • Author(s): Chen J
  • Journal Title: Int J Mol Med 24 Pages: 789-794
  • Peer Reviewed
• [Presentation] Symposium A global gene therapy renaissance Prostate cancer gene therapy in Japan-from HSV-tk to REIC/Dkk-3 (2010)
  • Author(s): Nasu Y
  • Organizer: American Society of Gene&Cell Therapy 13th Annual Meetings
  • Place of Presentation: Washington, DC, USA
• [Presentation] In situ gene therapy for prostate cancer-its local and systemic effect- (2009)
  • Author(s): 那須保友
  • Organizer: 日本遺伝子治療学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-09
• [Book] REIC/Dkk-3 gene therapy (2011)
  • Author(s): Watanabe M, Kaku H, Huang P, Kumon H, Nasu Y
  • Publisher: Nihon Rinsho
• [Book] Suicide gene and immuno gene therapy for prostate cancer (2011)
  • Author(s): Nasu Y, Sasaki K, Kumon H, Kaku H, Watanabe M
  • Publisher: Nihon Rinsho