Analysis of the molecules of cell polarity in associated with hormone

• Project/Area Number: 21390443
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Yokohama City University
• Principal Investigator: KUBOTA Yoshinobu 横浜市立大学, 医学研究科, 教授 (10106312)
• Co-Investigator(Kenkyū-buntansha): UEMURA Hiroji 横浜市立大学, 附属病院, 准教授 (50244439) ; NAGASHIMA Youji 横浜市立大学, 医学研究科, 准教授 (10217995) ; AKIMOTO Kazunori 横浜市立大学, 医学部, 助教 (70285104)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2009: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
• Keywords: 腫瘍学 / 細胞極性制御分子 / aPKCλ / IL-6 / aPKC λ

Research Abstract

The mechanisms of developing hormone refractory prostate cancer(CRPC) remains unclear. We have provided the evidence that aPKCλ/ιexpression correlates with prostate cancer recurrence. Experiments in vitro and in vivo revealed aPKCλ/ιto be involved in prostate cancer cell growth through secretion of IL-6.And we further demonstrated a correlation between biochemical recurrence after prostatectomy and the expression of these two factors. These results support our hypothesis that aPKCλ/ιplay an important role in prostate cancer progression.

Research Products

• [Journal Article] The co-expression of aPKCλ/ιand IL-6 in prostate cancer tissue correlates with biochemical recurrence (2011)
  • Author(s): Ishiguro H, Akimoto K, Nagashima Y, Kagawa E, Sasaki T, Sano JY, Takagawa R, Fujinami K, Sasaki K, Aoki I, Ohno S, Kubota Y, Uemura H
  • Journal Title: Cancer Science Volume: 102(8) Pages: 1576-81
• [Journal Article] Identification of phosphorylated proteins involved in the oncogenesis of prostate cancer via Pin1-proteomic analysis (2011)
  • Author(s): Endoh K, Nishi M, Ishiguro H, Uemura H, Miyagi Y, Aoki I, Hirano H, Kubota Y, Ryo A
  • Journal Title: The Prostate
• [Journal Article] Regulation of androgen receptor expression through angiotensin II type 1 receptor in prostate cancer cells (2011)
  • Author(s): Hoshino K, Ishiguro H, Teranishi JI, Yoshida SI, Umemura S, Kubota Y, Uemura H
  • Journal Title: The Prostate Volume: 71(9) Pages: 964-75
• [Journal Article] Engagement of renin-angiotensin system in prostate cancer (2011)
  • Author(s): Uemura H, Hoshino K, Kubota Y
  • Journal Title: Current Cancer Drug Targets Volume: 11(4) Pages: 442-450
• [Journal Article] Contrast-enhanced ultrasonography of the prostate : various imaging findings that indicate prostate cancer (2011)
  • Author(s): Sano F, Terao H, Kawahara T, Miyoshi Y, Sasaki T, Noguchi K, Kubota Y, Uemura H
  • Journal Title: BJU Intenational Volume: 107(9) Pages: 1404-10
• [Journal Article] The potential of FDG-PET/CT for detecting prostate cancer in patients with an elevated serum PSA level (2011)
  • Author(s): Minamimoto R, Uemura H, Sano F, Terao H, Nagashima Y, Yamanaka S, Shizukuishi K, Tateishi U, Kubota Y, Inoue T
  • Journal Title: Annals of Nuclear Medicine Volume: 25(1) Pages: 21-27
  • NAID: 10028998804
• [Journal Article] The co-expression of aPKCλ/ι and IL-6 in prostate cancer tissue correlates with biochemical recurrence (2011)
  • Author(s): Ishiguro H, Akimoto K, Nagashima Y, Kagawa E, Sasaki T, Sano J-y, Takagawa R, Fujinami K, Sasaki K, Aoki I, Ohno S, Kubota Y, Uemura H
  • Journal Title: Cancer Sci Volume: 102(8) Pages: 1576-1581
  • NAID: 10030242512
  • Peer Reviewed
• [Journal Article] Involvement of Runx3 in the basal transcriptional activation of the mouse angiotensin II type 1 receptor-associated protein gene (2011)
  • Author(s): Matsuda M, Tamura K, Wakui H, Dejima T, Maeda A, Ohsawa M, Kanaoka T, Haku S, Azushima K, Yamasaki H, Saito D, Hirose T, Maeshima Y, Nagashima Y, Umemura S
  • Journal Title: Physiol Genomics Volume: 43(14) Pages: 884-894
  • Peer Reviewed
• [Journal Article] A distinct role for Pin1 in the induction and maintenance of pluripotency (2011)
  • Author(s): Nishi M, Akutsu H, Masui S, Kondo A, Nagashima Y, Kimura H, Perrem K, Shigeri Y, Toyoda M, Okayama A, Hirano H, Umezawa A, Yamamoto N, Lee SW, Ryo A
  • Journal Title: J Biol Chem Volume: 286(13) Pages: 11593-11603
  • Peer Reviewed
• [Journal Article] Engagement of renin-angiotensin system in prostate cancer. (2011)
  • Author(s): Uemura H, Hoshino K, Kubota, Y
  • Journal Title: Current Cancer Drug Targets Volume: 11(4) Pages: 442-450
  • Peer Reviewed
• [Journal Article] Regulation of androgen receptor expression through angiotensin II type1 receptor in prostate cancer cells. (2011)
  • Author(s): Hoshino K, Ishiguro H, Teranishi JI, Yoshida SI, Umemura S, Kubota Y, Uemura H.
  • Journal Title: The Prostate Volume: 71(9) Pages: 964-975
  • Peer Reviewed
• [Journal Article] The co-expression of aPKCλ/ι and IL-6 in prostate cancer tissue correlates with biochemical recurrence. (2011)
  • Author(s): Ishiguro H, Akimoto K, Nagashima Y, Kagawa E, Sasaki T, Sano JY, Takagawa R, Fujinami K, Sasaki K, Aoki I, Ohno S, Kubota Y, Uemura H
  • Journal Title: Cancer Science Volume: (In press)
  • NAID: 10030242512
  • Peer Reviewed
• [Journal Article] Analysis of NSAID-activated gene 1 expression in prostate cancer (2010)
  • Author(s): Kawahara T, Ishiguro H, Hoshino K, Teranishi J, Miyoshi Y, Kubota Y, Uemura H
  • Journal Title: Urologia Internationalis Volume: 84(2) Pages: 198-202
• [Journal Article] High expression of atypical protein kinase C l/I in gastric cancer as a prognositic factor for recurrence (2010)
  • Author(s): Takagawa R, Akimoto K, Ichikawa Y, Akiyama H, Kojima Y, Ishiguro H, Inayama Y, Aoki I, Kunisaki C, Endo I, Nagashima Y, Ohno S
  • Journal Title: Annals of Surgical Oncology Volume: 17(1) Pages: 81-88
• [Journal Article] Analysis of NSAID-activated gene 1 expression in prostate cancer. (2010)
  • Author(s): Kawahara T, Ishiguro H, Hoshino K, Teranishi J, Miyoshi Y, kubota Y, Uemura H.
  • Journal Title: Urologia Internationalis Volume: 84(2) Pages: 198-202
  • Peer Reviewed
• [Journal Article] PKCλ/ιpromotes growth of prostate cancer cells in an autocrine manner through transcriptional activation of interleukin-6 (2009)
  • Author(s): Ishiguro H, Akimoto K, Nagashima Y, Kojima Y, Sasaki Y, Ishiguro-Imagawa Y, Nakaigawa N, Ohno S, Kubota Y, Uemura H
  • Journal Title: Proceedings of the National Academy of Sciences(PNAS) Volume: 106(38) Pages: 16369-16374
• [Journal Article] Avid F-18 FDG uptake in prostatic sarcoma (2009)
  • Author(s): Minamimoto R, Tateishi U, Uemura H, Yamanaka S, Kubota Y, Inoue T
  • Journal Title: Clinical Nuclear Medicine Volume: 34(6) Pages: 388-389
• [Journal Article] A cell polarity protein aPKClambda is required for eye lens formation and growth (2009)
  • Author(s): Sugiyama Y, Akimoto K, Robinson M. L, Ohno S, Quinlan R. A
  • Journal Title: Dev Biol Volume: 336(2) Pages: 246-256
• [Journal Article] An essential role of the universal polarity protein, aPKClambda, on the maintenance of podocyte slit diaphragms (2009)
  • Author(s): Hirose T, Satoh D, Kurihara H, Kusaka C, Hirose H, Akimoto K, Matsusaka T, Ichikawa I, Noda T, Ohno S
  • Journal Title: PLoS ONE Volume: 4(1)
• [Journal Article] aPKCλ/1 promotes growth of prostate cancer cells in an autocrine manner through transcriptional activation of interleukin-6 (2009)
  • Author(s): Ishiguro H, Kubota Y, et al.
  • Journal Title: Proceedings of the National Academy of Sciences(PNAS) 106(38) Pages: 16369-16374
  • Peer Reviewed
• [Journal Article] A cell polarity protein aPKClambda is required for eye lens formation and growth (2009)
  • Author(s): Sugiyama Y, Akimoto K, et al.
  • Journal Title: Developmental Biology 336(2) Pages: 246-256
  • Peer Reviewed
• [Presentation] Salvage radiotherapy for rising PSA after radical prostatectomy (2010)
  • Author(s): Iwasaki M, Uemura H, Nakaigawa N, Hayashi N, Ueno D, Makiyama K, Sano F, Sakata R, Noguchi G, Kawakami S, Hata M, Omura M, Inoue T, Kubota Y
  • Organizer: The 20th Taiwan Urology Association Annual Meeting
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2010-11-13
• [Presentation] In laterally spreading type tumors(LSTs) of the colon or the rectum, expression of the atypical protein kinase C lambda/iota is correlated to expression of beta-catenin and type IV collagen (2010)
  • Author(s): Ichikawa Y, Ishikawa T, Shimizu D, Goto A, Shimamura T, Suwa H, Tatsumi K, Watanabe K, Ota M, Fujii S, Kawamata M, Akimoto K, Nagashima Y, Takahashi H, Nakajima A, Ohno S, Endo I
  • Organizer: The 102nd Annual Meeting of American Assiciation for Cancer Research,(Orland, FL)
  • Place of Presentation: Orland, FL
  • Year and Date: 2010-09-22
• [Presentation] Role of Renin-Angiotensin System in Prostate Cancer. Symposium "Angiotensin" (2010)
  • Author(s): Uemura H, Takahashi S, Hoshino K, Ishiguro H, Kubota Y
  • Organizer: 14th International Congress on Hormone Steroids and Hormones & Cancer
  • Place of Presentation: Edinburgh, England
  • Year and Date: 2010-09-22
• [Presentation] Role of renin-angiotensin system in prostate cancer (2010)
  • Author(s): Uemura H, Takahashi S, Hoshino K, Ishiguro H, Kubota Y
  • Organizer: Symposium "Angiotensin", 14th International Congress on Hormone Steroids and Hormones & Cancer.
  • Place of Presentation: イギリス, エジンバラ, University of Edinburgh John McIntyre Conference Centre
  • Year and Date: 2010-09-22
• [Presentation] Atypical protein kinase Cλ/ιregulates mouse mammary progenitor cell propagation. (2010)
  • Author(s): Akimoto K, Zheng Y-W, Nagashima Y, Ishiguro H, Asai-Sato M, Kim M, Taniguchi H, Noda T, Ohno S., et al.
  • Organizer: 6th International Symposium on Hormonal Oncogenesis
  • Place of Presentation: 千葉県浦安市, シェラトン・グランデ・トーキョーベイホテル
  • Year and Date: 2010-09-13
• [Presentation] Atypical protein kinase Cλ/ιregulates mouse mammary progenitor cell propagation (2010)
  • Author(s): Akimoto K, Zheng Y-W, Nagashima Y, Ishiguro H, Asai-Sato M, Kojima Y, Li B, Sasaki K, Kagawa E, Kim M, Taniguchi H, Noda T, and Ohno S
  • Organizer: 6th International Symposium on Hormonal Oncogenesis
  • Place of Presentation: Tokyo
• [Presentation] 前立腺癌におけるaPKCの発現と前立腺癌進展機構 (2009)
  • Author(s): 石黒斉, 窪田吉信, 他
  • Organizer: 第97回日本泌尿器科学会総会
  • Place of Presentation: 岡山
  • Year and Date: 2009-04-16
• [Presentation] Deter mination of Androgens concentrations in prostate tissue taken by needle biopsy by LC/MS/MS. Preliminary study for diagnosis of malignancy from androgen level in prostate tissue (2009)
  • Author(s): Miyoshi Y, Umemoto S, Uemura H, Shibata Y, Arai S, Suzuki K, Honma S, Kubota Y
  • Organizer: American Urological Association 104th Annual Meeting
  • Place of Presentation: Chicago, USA
• [Book] 翻訳後修飾のプロテオミクス3章翻訳後修飾解析のための試料調整3.2.組織薄切片からの試料調整 (2011)
  • Author(s): 秋本和憲、永坂恵子、岡山明子、川上隆雄
  • Publisher: 講談社サイエンティフィク
• [Book] 6章翻訳後修飾の機能解析の方法6.8遺伝子操作マウスを用いた個体レベルでの機能解析 (2011)
  • Author(s): 秋本和憲、大野茂男
  • Publisher: 講談社サイエンティフィク翻訳後修飾のプロテオミクス