| Project/Area Number |
21390448
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
|
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| Research Institution | Gunma University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IGARASHI Shigeo 群馬大学, 医学部, 講師 (60343084)
KISHI Hiroshi 群馬大学, 医学部, 講師 (10375545)
ITO Masahiro 群馬大学, 医学部, 准教授 (20282402)
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Keywords | miRNA / LH receptor / down regulation / 卵巣 / microRNA / micro RNA / シグナル伝達 |
|---|
| Research Abstract |
MicroRNAs (miRNAs) are small non-coding RNAs that interact with mRNAs and trigger either translation repression or RNA cleavage of target genes. In this study, we investigated whether miRNA is involved in down-regulation of the LH receptor (LHR) in the ovary. A miRNA microarray was carried out to analyze the overall miRNA expression profile while LHR mRNA was down-regulated, and found that 23 miRNAs were highly expressed during this period. Combining these results with data from the bioinformative database, the clustering analysis led us to focus on miR-136* for further analysis. In both in vivo and in vitro studies, miR-136* levels were found to increase 6 hr after hCG administration while LHR mRNA levels were down-regulated. To confirm that miR-136* binds to LHR mRNA, we cloned the 3'-end of LHR mRNA from 2570 to 2895 into reporter vector which contained a Renilla luciferase coding region upstream of the cloning site. When the miR-136* inhibitor was co-transfected with the reporter vector in granulosa cells, the luciferase activity was de-repressed, revealing that miR-136* definitely bound to the 3'-end of LHR mRNA. From these data, we conclude that miR-136* participates in the mechanism of down-regulation of LHR mRNA, whereby miR-136* forms base pairs with LHR mRNA.
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