• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Clinical application of human ovarian tissue cryopreservation

Research Project

Project/Area Number 21390455
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKeio University

Principal Investigator

KUJI Naoaki  慶應義塾大学, 医学部, 講師 (80169987)

Co-Investigator(Kenkyū-buntansha) SUZUKI Hiroshi  帯広畜産大学, 原虫病研究センター, 教授 (60333473)
KASHIWAZAKI Naomi  麻布大学, 獣医学部, 教授 (90298232)
Co-Investigator(Renkei-kenkyūsha) HAMATANI Toshio  慶應義塾大学, 医学部, 講師 (60265882)
MOCHIMARU Yoshiyuki  慶應義塾大学, 医学部, 助教 (80445238)
TAKANO Mitsuko  慶應義塾大学, 医学部, 助教 (20445240)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywords移植・再生医療 / 医療・福祉 / 再生医学
Research Abstract

The efficacy of magnetic field supplementation was confirmed, since normal litter was born only from magnetic field supplemented cryopreserved ovarian tissue, and those litter were appeared to be fertile. Although ovaries from juvenile porcine were cryopreserved, there is no ovary with function after freeze-thaw, neither from cryopreservation with magnetic field supplementation or that without magnetic field supplementation. Freezing temperature of both distilled water and freezing medium was not moved by magnetic field supplementation.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (41 results)

All 2011 2010 2009

All Journal Article (24 results) (of which Peer Reviewed: 18 results) Presentation (17 results)

  • [Journal Article] Effect of bovine cumulus-oocyte complexes-conditioned medium on in-vitro maturation of canine oocytes2011

    • Author(s)
      Abdel-Ghani, M., Abe, Y., Asano, T., Hamano, S. and Suzuki, H.
    • Journal Title

      Reprod. Med. Biol

      Volume: 10 Pages: 43-49

    • NAID

      10029042897

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cryopreservation of canine embryos2011

    • Author(s)
      Abe, Y., Suwa, Y., Asano, T., Ueta, Y. Y., Kobayashi, N., Ohshima, N., Shirasuna, S., Abdel-Ghani, M. A., Oi, M., Kobayashi, Y., Miyoshi, M., Miyahara, K. and Suzuki, H.
    • Journal Title

      Biol. Reprod

      Volume: 84 Pages: 363-368

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of graft site and gonadotrophin treatment on follicular development of canine ovarian grafts transplanted to NOD-SCID mice2011

    • Author(s)
      Kawase, Y. and Suzuki, H
    • Journal Title

      Reprod. Med. Biol

      Volume: 10 Pages: 259-266

    • NAID

      10030444416

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of intraperitoneal administration of desialylated erythropoietin on the follicul ar survival in cryopreserved canine ovaries after xenotransplantation2011

    • Author(s)
      Hirayama, Y., Inoue, K. and Suzuki, H.
    • Journal Title

      J. Mamm. Ova Res

      Volume: 28 Pages: 143-147

    • NAID

      10029898554

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expr ession of erythropoietic cytokines in alpha-tocopherol tran sfer protein knockout mice with murine malaria infection2011

    • Author(s)
      Herbas, M. S., Ueta, Y. Y., Ishibashi, K. and Suzuki, H.
    • Journal Title

      Parasitol. Res

      Volume: 109 Pages: 1243-1250

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sero epidemiological survey of Brucella canis infection in dogs in Japan using the tube agglutination test2011

    • Author(s)
      Tachibana, M., Kobayashi, N., Inokuma, H., Suzuki, H. and Watarai, M.
    • Journal Title

      J. Jpn. Vet. Med. Assoc

      Volume: 64 Pages: 559-561

    • NAID

      10029384292

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Beneficial effect of hyperbaric oxygen therapy on the follicular survival of mouse ovary after transplantation2011

    • Author(s)
      Hayashi, S., Asano, T., Kakizaki, R. and Suzuki, H.
    • Journal Title

      J. Reprod. Dev

      Volume: 58(In press)

    • NAID

      10030754171

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Scavenger receptor mediates systemic RNA interference in ticks2011

    • Author(s)
      Aung, K. M., Boldbaatar, D., Umemiya-Shira fuji, R., Liao, M., Xuan, X., Suzuki, H., Galay, R. L., Tanaka, T. and Fujisaki, K.
    • Journal Title

      PLoS ONE

      Volume: 6(12)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro maturation of canine oocytes co-cultured with bovine and canine granulosa cell monolayers2011

    • Author(s)
      Abdel-Ghani, M., Shimizu, T., Asano, T. and Suzuki, H
    • Journal Title

      Theriogenology

      Volume: 77 Pages: 347-355

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 【不妊診療のすべて】ART(生殖補助医療)精子・卵子・卵巣の凍結保存とその安全性2011

    • Author(s)
      久慈直昭, 井上治, 福永朝子, 菅原かな, 小川誠司, 奥村典子, 山田満稔, 浜谷敏生, 吉村泰典
    • Journal Title

      産婦人科治療

      Volume: 102巻 Pages: 495-500

    • Related Report
      2011 Final Research Report
  • [Journal Article] Effect of bovine cumulus-oocyte complexes-conditioned medium on in-vitro maturation of canine oocytes2011

    • Author(s)
      Abdel-Ghani, M., Abe, Y., Asano, T., Hamano, S., Suzuki, H.
    • Journal Title

      Reprod.Med.Biol.

      Volume: 10 Pages: 43-49

    • NAID

      10029042897

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cryopreservation of canine embryos2011

    • Author(s)
      Abe, Y., Suwa, Y., Asano, T., Ueta, Y.Y., Kobayashi, N., Ohshima, N., Shirasuna, S., Abdel-Ghani, M.A., Oi, M., Kobayashi, Y., Miyoshi, M., Miyahara, K., Suzuki, H.
    • Journal Title

      Biol.Reprod.

      Volume: 84 Pages: 363-368

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A study on freeze-drying as a method of preserving mouse sperm2011

    • Author(s)
      Kawase, Y., Suzuki, H.
    • Journal Title

      J.Reprod.Dev.

      Volume: 57 Pages: 176-182

    • NAID

      10028274982

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of graft site and gonadotrophin treatment on follicular development of canine ovarian grafts transplanted to NOD-SCID mice2011

    • Author(s)
      Abdel-Ghani, M., Abe, Y, Asano, T., Suzuki, H.
    • Journal Title

      Reprod.Med.Biol.

      Volume: 10 Pages: 259-266

    • NAID

      10030444416

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of intraperitoneal administration of desialylated erythropoietin on the follicular survival in cryopreserved canine ovaries after xenotransplantation2011

    • Author(s)
      Hirayama, Y., Inoue, K., Suzuki, H.
    • Journal Title

      J.Mamm.Ova Res.

      Volume: 28 Pages: 143-147

    • NAID

      10029898554

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of erythropoietic cytokines in alpha-tocopherol transfer protein knockout mice with murine malaria infection2011

    • Author(s)
      Herbas, M.S., Ueta, Y.Y., Ishibashi, K., Suzuki, H.
    • Journal Title

      Parasitol.Res.

      Volume: 109 Pages: 1243-1250

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seroepidemiological survey of Brucella canis infection in dogs in Japan using the tube agglutination test2011

    • Author(s)
      Tachibana, M., Kobayashi, N., Inokuma, H., Suzuki, H., Watarai, M.
    • Journal Title

      J.Jpn.Vet.Med.Assoc.

      Volume: 64 Pages: 559-561

    • NAID

      10029384292

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Beneficial effect of hyperbaric oxygen therapy on the follicular survival of mouse ovary after transplantation2011

    • Author(s)
      Hayashi, S., Asano, T., Kakizaki, R., Suzuki, H.
    • Journal Title

      J.Reprod.Dev.

      Volume: 58(In press)

    • NAID

      10030754171

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Scavenger receptor mediates systemic RNA interference in ticks2011

    • Author(s)
      Aung, K.M., Boldbaatar, D., Umemiya-Shirafuji, R., Liao, M., Xuan, X., Suzuki, H., Galay, R.L., Tanaka, T., Fujisaki, K.
    • Journal Title

      PLoS ONE

      Volume: 6(12)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 【不妊診療のすべて】ART(生殖補助医療)精子・卵子・卵巣の凍結保存とその安全性2011

    • Author(s)
      久慈直昭(慶応義塾大学医学部産婦人科学), 井上治, 福永朝子, 菅原かな, 小川誠司, 奥村典子, 山田満稔, 浜谷敏生, 吉村泰典
    • Journal Title

      産婦人科治療

      Volume: 102巻増刊 Pages: 495-500

    • Related Report
      2011 Annual Research Report
  • [Journal Article] マウス単一卵子のDNAマイクロアレイによる遺伝子発現解析とその応用2010

    • Author(s)
      持丸佳之、久慈直昭、山田満稔、奥村典子、高野光子、浜谷敏生, 他5名
    • Journal Title

      日本産科婦人科学会雑誌

      Volume: 62(2) Pages: 344-344

    • Related Report
      2011 Final Research Report
  • [Journal Article] 精子・卵子・卵巣の凍結保存とその安全性2010

    • Author(s)
      久慈直昭
    • Journal Title

      産婦人科治療

      Volume: 101 Pages: 427-433

    • NAID

      110007731031

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 精子・卵子・卵巣の凍結保存とその安全性2010

    • Author(s)
      久慈直昭
    • Journal Title

      日本産科婦人科学会雑誌

      Volume: 62

    • NAID

      110007731031

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 交流磁場発生装置を用いたブタ卵巣の超低温保存2009

    • Author(s)
      持丸佳之、久慈直昭、高野光子、山田満稔、浜谷敏生, 他3名
    • Journal Title

      日本産科婦人科雑誌

      Volume: 61(2) Pages: 635-635

    • Related Report
      2011 Final Research Report
  • [Presentation] X、Y精子選別によるイヌの雌雄産み分け技術の開発-FISH法によるイヌ精子の性判別法の確立-2011

    • Author(s)
      Oi, M., Yamada, K., Hayakawa, H. and Suzuki, H.
    • Organizer
      第26回動物生殖工学研究会
    • Place of Presentation
      北里本館,東京
    • Year and Date
      2011-12-04
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 盲導犬適性と性格関連遺伝子多型との関連性2011

    • Author(s)
      Ohshima, N., Kobayashi, N., Shirasuna, S., Suwa, Y. and Suzuki, H.
    • Organizer
      北海道実験動物研究会・日本実験動物技術者協会北海道支部合同学術集会
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2011-07-09
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] マウスの卵巣移植における高圧酸素療法の効果2011

    • Author(s)
      Hayashi, S., Asano, T. and Suzuki, H.
    • Organizer
      北海道実験動物研究会・日本実験動物技術者協会北海道支部合同学術集会
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2011-07-09
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Puppies from frozen embryos2011

    • Author(s)
      Suzuki, H
    • Organizer
      第104回日本繁殖生物学会
    • Place of Presentation
      いわて県民情報交流センター・アイーナ,盛岡(岩手県)
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Murine Plasmodium virulence is affected due to oxidative stress status of the host2011

    • Author(s)
      Herbas, S. M., Maggloire, N. and Suzuki, H.
    • Organizer
      17^<th> Japanese-German cooperative symposium on protozoan diseases
    • Place of Presentation
      Nara Women's University, Nara
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Follicular loss of cryopreserved canine ovary after xenotransplantation and its solution2011

    • Author(s)
      Suzuki, H
    • Organizer
      The 12th International Congress on Reproductive Biomedicine(招聘講演)
    • Place of Presentation
      Royan Institute, Tehran, The Islamic Republic of Iran
    • Related Report
      2011 Final Research Report
  • [Presentation] Cryopreservation of canine embryos2011

    • Author(s)
      Suzuki, H
    • Organizer
      The 12th International Congress on Reproductive Biomedicine(招聘講演)
    • Place of Presentation
      Royan Institute, Tehran, The Islamic Republic of Iran
    • Related Report
      2011 Final Research Report
  • [Presentation] Effect of hyperbaric oxygen therapy on the follicular loss of ovarian tissues after transplantation2011

    • Author(s)
      Suzuki, H., Hayashi, S. and Asano, T.
    • Organizer
      44th Annual Meeting of the Society for the Study of Reproduction
    • Place of Presentation
      Portland, Oregon, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Processing of semen from an HIV-1-positive male and its use in the IVF-ICSI procedure-clinical efficacy2011

    • Author(s)
      Osamu INOUE, Naoaki KUJI, Tomoko FUKUNAGA, Seiji OGAWA, Kana SUGAWARA, Mitsutoshi YAMADA, Toshio HAMATANI, Hideji HANABUSA, Yasunori YOSHIMURA and Shingo KATO
    • Organizer
      27th Annual Meeting of the European Society of Human Reproductoin and Embryology
    • Place of Presentation
      Stockholm, Sweden
    • Related Report
      2011 Final Research Report
  • [Presentation] マウスの卵巣移植における高圧酸素療法の卵胞数減少抑制効果について2011

    • Author(s)
      Hayashi, S., Asano, T. and Suzuki, H.
    • Organizer
      第52回日本哺乳動物卵子学会
    • Place of Presentation
      国際医療福祉大学本校
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Effect of hyperbaric oxygen therapy on the follicular loss of ovarian tissues after transplantation2011

    • Author(s)
      Suzuki. H., Hayashi, S., Asano, T.
    • Organizer
      44th Annual Meeting of the Society for the Study of Reproduction
    • Place of Presentation
      Portland, Oregon, USA(招聘講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cryopreservation of canine embryos2011

    • Author(s)
      Suzuki, H.
    • Organizer
      the 12th International Congress on Reproductive Biomedicine
    • Place of Presentation
      Royan Institute, Tehran, The Islamic Republic of Iran(招聘講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Follicular loss of cryopreserved canine ovary after xenotransplantation and its solution2011

    • Author(s)
      Suzuki, H.
    • Organizer
      the 12th International Congress on Reproductive Biomedicine
    • Place of Presentation
      Royan Institute, Tehran, The Islamic Republic of Iran(招聘講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] マウス単一卵子のDNAマイクロアレイによる遺伝子発現解析とその応用2010

    • Author(s)
      持丸佳之, 久慈直昭, 他
    • Organizer
      第62回 日本産科婦人科学会学術講演会
    • Place of Presentation
      東京・東京国際フォーラム
    • Year and Date
      2010-04-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 単一卵子のDNAマイクロアレイによる遺伝子発現解析とその応用2009

    • Author(s)
      持丸佳之, 久慈直昭, 他
    • Organizer
      日本受精着床学会
    • Place of Presentation
      京都国際会館 京都市
    • Year and Date
      2009-08-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] Globalgene expression analysis in single oocytes from young and aged mice2009

    • Author(s)
      Naoaki Kuji, et al
    • Organizer
      European Society of Human Reproduction and Embryology
    • Place of Presentation
      Amstedam, Holland
    • Year and Date
      2009-07-01
    • Related Report
      2011 Final Research Report
  • [Presentation] Global gene expression analysis in single oocytes from young and aged mice2009

    • Author(s)
      久慈直昭, et al.
    • Organizer
      European Society of Human Reproduction and Embryology
    • Place of Presentation
      Amstedam, Holland
    • Year and Date
      2009-07-01
    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi