Regenerative medicine for pediatric surgical diseases using stem cells with their improved microenvironments.

• Project/Area Number: 21390472
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: The University of Tokyo (2011-2012); University of Tsukuba (2009-2010)
• Principal Investigator: KOMURO Hiroaki 東京大学, 医学部付属病院, 准教授 (80296128)
• Co-Investigator(Renkei-kenkyūsha): CHEN Guoping 独立行政法人物質・材料研究機構, 主任研究者 (50357505) ; TABATA Yasuhiko 京都大学, 再生医科学研究所, 教授 (50211371)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 鎖肛 / 排便 / 骨格筋 / 再生 / 筋芽細胞移植 / bFGF / マイクロパターニング / 幹細胞 / 骨格筋再生 / 排便機能 / 骨格筋関連遺伝子 / ｂFGF / 気管 / 軟骨 / 羊膜 / 神経細胞

Research Abstract

Infants with higher anorectal anomalies often develop fecal incontinence after surgical reconstruction mainly due to the incomplete development of defecation muscles. We investigated the possibility of defecation muscle regeneration by myoblast transplantation to improve fecal continence. The efficacy of myoblast transplantation in combination with the controlled and sustained delivery of basic fibroblast growth factor (bFGF) is investigated. The controlled and sustained release of bFGF from gelatin hydrogel microspheres improves the survival rate of transplanted myoblasts and promotes muscle regeneration by facilitating myogenesis rather than angiogenesis. Myoblast transplantation coupled with growth factors may provide a novel treatment strategy for improving fecal continence after repair of anorectal anomalies in infants.

Research Products

• [Journal Article] Promotion of muscle regeneration by myoblast transplantation using the controlled delivery system of bFGF (2013)
  • Author(s): Hagiwara K, Chen G, Kawazoe N, Tabata Y, Komuro H
  • Journal Title: Journal of Tissue Engineering and Regenerative Medicine Volume: Epub ahead of print Issue: 4 Pages: 289-293
  • DOI: 10.1002/term.1732
  • Peer Reviewed
• [Journal Article] Slow release of basic fibroblast growth factor (b-FGF) promotes growth of tracheal cartilage (2013)
  • Author(s): Ishimaru T, Komura M, Komura H, Otani Y, Komuro H, Sugiyma M, Terawaki K, Suzuki K, Tabata Y, Iwanaka T
  • Journal Title: J Pediatr Surg Volume: 48 Pages: 288-292
• [Journal Article] Epithelial mesenchymal transition-related gene expression as a new prognostic marker for neuroblastoma. International (2013)
  • Author(s): Nozato M, Kaneko S, Nakagawara A, Komuro H
  • Journal Title: Journal of Oncology Volume: 42 Pages: 134-140
• [Journal Article] 再生医療の小児外科領域への展開 (2010)
  • Author(s): 小室広昭
  • Journal Title: 日本周産期・新生児医学会雑誌 Volume: 46(4) Pages: 985-988
  • NAID: 10027853318
• [Journal Article] 特集 再生医療用多孔質材料の研究開発動向:先天性奇形に対する多孔質材料を用いた膀胱・横隔膜の再生 (2010)
  • Author(s): 小室広昭
  • Journal Title: 化学工業 Volume: 61 Pages: 50-55
• [Journal Article] Neural differentiation potential of rat amniotic epithelial cells (2010)
  • Author(s): Shinya M, Komuro H, Saihara R, Urita Y, Kaneko S, Kaneko M, Liu Y
  • Journal Title: Fetal and Pediatric Pathology Volume: 29 Pages: 133-143
• [Journal Article] Tracheal defect repair using a PLGA-collagen hybrid scaffold reinforced by a copolymer stent with bFGF-impregnated gelatin hydrogel. Pediatric (2010)
  • Author(s): Tatekawa Y, Kawazoe N, Chen G, Shirasaki Y, Komuro H, Kaneko M
  • Journal Title: Surgery International Volume: 26 Pages: 575-580
• [Journal Article] Experimental Repair of Tracheal Defect Using a Bioabsorbable Copolymer (2010)
  • Author(s): Tatekawa Y, Ikada Y, Komuro H, Kaneko M
  • Journal Title: Journal of Surgical Research Volume: 160 Pages: 114-121
• [Journal Article] Neural differentiation potential of rat amniotic epithelial cells. (2010)
  • Author(s): Shinya M, Komuro H
  • Journal Title: Fetal and Pediatric Pathology Volume: 29 Pages: 133-143
  • Peer Reviewed
• [Journal Article] Tracheal defect repair using a PLGA-collagen hybrid scaffold reinforced by a copolymer stent with bFGF-impregnated gelatin hydrogel. (2010)
  • Author(s): Tatekawa Y, Komuro H, et al
  • Journal Title: Pediatric Surgery International Volume: 26 Pages: 575-580
  • Peer Reviewed
• [Journal Article] Experimental Repair of Tracheal Defect Using a Bioabsorbable Copolymer. (2010)
  • Author(s): Tatekawa Y, Komuro H, et al
  • Journal Title: Journal of Surgical Research Volume: 160 Pages: 114-121
  • Peer Reviewed
• [Journal Article] 先天性奇形に対する多孔質材料を用いた膀胱・横隔膜の再生 (2010)
  • Author(s): 小室広昭
  • Journal Title: 化学工業 Volume: 61 Pages: 50-55
• [Journal Article] Neural differentiation potential of rat amniotic epithelial cells. (2010)
  • Author(s): Shinya M, Komuro H
  • Journal Title: Fetal and Pediatric Pathology (In press)(印刷中)
  • Peer Reviewed
• [Journal Article] transplantation to defecation muscles in rats: A possible treatment strategy for fecal incontinence after the repair of anorectal anomalies (2009)
  • Author(s): Saihara R, Komuro H, Urita Y, et al.Myoblast
  • Journal Title: Pediatr. Surg. Int Volume: 25 Pages: 981-986
• [Journal Article] Myoblast transplantation to defecation muscles in rats : A possible treatment strategy for fecal incontinence after the repair of anorectal anomalies. (2009)
  • Author(s): Saihara R, Komuro H
  • Journal Title: Pediatric Surgery International 25 Pages: 981-986
  • Peer Reviewed
• [Presentation] Gelatin hydrogel microsphere を用いたbFGF徐放による筋芽細胞移植 の骨格筋再生効果の促進 (2013)
  • Author(s): 小室広昭、萩原幸輝、陳国平、田畑泰彦、岩中督
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-22
• [Presentation] ゼラチンハイドロゲルを用いたbFGF徐放による筋芽細胞移植の骨格筋再生効果の促進 (2012)
  • Author(s): 小室広昭、萩原幸輝、岩中督
  • Organizer: 第49回日本小児外科学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2012-05-14
• [Presentation] bFGF徐放と筋芽細胞移植の併用による骨格筋再生効果 (2011)
  • Author(s): 萩原幸輝、田畑泰彦、陳国平、小室広昭
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-02
• [Presentation] 先天性奇形にたいする組織工学を用いた治療戦略。シンポジウム「組織 3 次元構築を目指した組織工学」 (2011)
  • Author(s): 小室広昭 瓜田泰久 萩原幸輝、陳国平、川添直輝
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-01
• [Presentation] 先天性奇形にたいする組織工学を用いた治療戦略 (2011)
  • Author(s): 小室広昭
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-01
• [Presentation] Promotion of muscle repair by myoblast transplantation using the controlled delivery system of bFGF (2010)
  • Author(s): Koki Hagiwara, Guoping Chen, Naoki Kawazoe, Hiroshi Kohara, Yasuhiko Tabata, Hiroaki Komuro
  • Organizer: Tissue Engineering and Regenerative Medicine International Society 2010Asia-Pacific Meeting
  • Place of Presentation: Sydney, Austlaria
  • Year and Date: 2010-09-17
• [Presentation] Promotion of muscle repair by myoblast transplantation using thecontrolled delivery system of bFGF. (2010)
  • Author(s): Kouki Hagiwara, Hiroaki Komuro
  • Organizer: TERMIS 2010 Asia-Pacific Congress
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2010-09-17
• [Presentation] Aligned myotubes on polyvinyl alcohol micro patterned plates in vitro. Tissue Engineering and Regenerative Medicine International (2010)
  • Author(s): Koki Hagiwara, Song Wei, Naoki Kawazoe, Guoping Chen, Hiroaki Komuro
  • Organizer: Society 2010 Asia-Pacific Meeting
  • Place of Presentation: Sydney, Austlaria
  • Year and Date: 2010-09-15
• [Presentation] Aligned myotubes on polyvinyl alcohol micro patterned plates in vitro. (2010)
  • Author(s): Kouki Hagiwara, Hiroaki Komuro
  • Organizer: TERMIS 2010 Asia-Pacific Congress
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2010-09-15
• [Presentation] Myoblast transplantation to defecation muscles in rats: A possible treatment strategy for fecal incontinence after the repair of anorectal anomalies (2009)
  • Author(s): Hiroaki Komuro, Ryoko Saihara, Kouki Hagiwara, Yasuhisa Urita, Michio Kaneko
  • Organizer: 2^<nd> TERMIS World Congress
  • Place of Presentation: Seoul
  • Year and Date: 2009-09-03
• [Presentation] Myoblast transplantation to defecation muscles in rats : A possible treatment strategy for fecal incontinence after the repair of anorectal anomalies. (2009)
  • Author(s): 小室広昭
  • Organizer: 2^<nd> TERMIS World Congress
  • Place of Presentation: Seoul(Korea)
  • Year and Date: 2009-09-03
• [Presentation] 筋由来の幹細胞を用いた排便筋の再生-鎖肛術後の 排便機能の改善に向けて- (2009)
  • Author(s): 小室広昭、才原良子、萩原幸輝、瓜田泰久、金子節子、金子道夫
  • Organizer: 第46回日本小児外科学会学術集会、シンポジウム「小児外科における先進医療」
  • Place of Presentation: 大阪
  • Year and Date: 2009-06-03
• [Presentation] 筋由来の幹細胞を用いた排便筋の再生-鎖肛術後の排便機能の改善に向けて- (2009)
  • Author(s): 小室広昭
  • Organizer: 第46回日本小児外科学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-06-03
• [Presentation] Myoblast transplantation to defecation muscles in rats: A possible treatment strategy for fecal incontinence after the repair of anorectal anomalies? (2009)
  • Author(s): Hiroaki Komuro, Ryoko Saihara, Kouki Hagiwara, Yasuhisa Urita, Michio Kaneko
  • Organizer: The 42^<th> Annual Meeting of the Pacific Association of Pediatric Surgeons
  • Place of Presentation: Hong Kong
  • Year and Date: 2009-05-11
• [Presentation] ゼラチンハイドロゲルを用いたbFGF徐放による筋芽細胞移植の骨格筋再生効果の促進。
  • Author(s): 小室広昭
  • Organizer: 第49回日本小児外科学会学術集会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Gelatin hydrogel microsphereを用いたbFGF徐放による筋芽細胞移植の骨格筋再生効果の促進
  • Author(s): 小室広昭
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 医工連携による再生医療の小児外科領域への展開
  • Author(s): 小室広昭
  • Organizer: つくば医工連携フォーラム2012
  • Place of Presentation: つくば市
  • Invited
• [Book] Tissue engineering and regenerative medicine for treatment of congenital birth defects, Advances in Medicine and (2010)
  • Author(s): Hiroaki Komuro
  • Publisher: Leon V. Berhardt (Eds)
• [Book] Tissue engineering and regenerative medicine for treatment of conge nital birth defects (2010)
  • Author(s): Hiroaki Komuro
  • Publisher: Nova Science Publishers Inc, New York At press
• [Book] Tissue engineering and regenerative medicine for treatment of congenital birth defects (2010)
  • Author(s): Hiroaki Komuro
  • Publisher: Nova Science Publishers Inc, New York(印刷中)