| Project/Area Number |
21390474
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
HIYAMA Eiso 広島大学, 自然科学研究支援開発センター, 教授 (00218744)
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| Co-Investigator(Kenkyū-buntansha) |
MASUJIMA Tsutomu 広島大学, 医歯薬学総合研究科, 教授 (10136054)
SOTOMARU Yusuke 広島大学, 自然科学研究支援開発センタ, 教授 (90309352)
KAMIMATSUSE Arata 広島大学, 病院, 病院助教 (90569881)
YAMAOKA Hiroaki 広島大学, 病院, 講師 (90311810)
HIYAMA Keiko 広島大学, 原爆放射線医科学研究所, 准教 (60253069)
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| Co-Investigator(Renkei-kenkyūsha) |
00TAKI Megu 広島大学, 原爆放射線医科学研究所, 教授 (20110463)
NAKAGAWARA Akira 千葉県がんセンター(研究所), 研究局, 局長 (50117181)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2009: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | 癌 / ゲノム / 細胞・組織 / 発生・分化 / トランスレーショナルリサーチ / がん幹細胞 / 神経芽腫 / 肝芽腫 / プロテオーム / マイクロアレイ / マイクロRNA / 小児がん / 一細胞解析 |
|---|
| Research Abstract |
In childhood cancers, the tumors derived from abnormal differentiation show favorable outcomes, while highly malignant tumors contain cancer stem cells and have a high frequency of relapse. In the present study, we isolated cancer stem cells from neuroblastoma and hepatoblastoma samples and analyzed their characteristics using genomics and one cell proteomics(Cellomics). In these cancer stem cells, neurodifferentiative pathway and glutamate metabolism were inhibited but several molecules including Bim-1 and Notch-1 were up-regulated. Among these molecules, we identified the molecule which was elevated in serum of the cases with unfavorable outcomes. This molecule is considered as a useful candidate for detecting cancer stem cells in childhood cancer patients
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