|Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Using transgenic mice that enabled us to trace NC cells and mesodermal cells as YFP^+ cells, respectively, we established ES cell lines to investigate whether NC cells or mesodemal cells were generated from ESCs in the culture. We found that NC-derived melanocytes were efficiently generated from NC-derived YFP^+ cells. The NC-derived YFP^+ cells also expressed strongly NC-associated genes. We also found that NC-derived YFP^+ cells differentiated into not only melanocytes, but also neurons, adipocytes, and osteoblasts. Furthermore, to detect the differentiation of odontoblasts in vivo and in vitro, we made Dspp Knock-In ES (Dspp-KI ES) cell lines by targeting vector that inserted GFP into Dspp gene. Then, Dspp-KI ES cells were injected into blastocysts, and large numbers of chimera mice were obtained. Dspp-KI ES-derived cells cultured for 6 days also were transplanted into kidney capsule, and 4 weeks after YFP^+ cells were detected in the transplanted tissues. We further try to differentiate these ESCs into odontoblasts in vitro through NC.