Induction of maxilla-facial bone with tooth from human induced pluripotent stem cells

• Project/Area Number: 21390493
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: National Institute of Biomedical Innovation
• Principal Investigator: FURUE Miho 独立行政法人医薬基盤研究所, 難病・疾患資源研究部, 研究リーダー (80257310)
• Co-Investigator(Kenkyū-buntansha): KOHARA Arihiro 独立行政法人医薬基盤研究所, 難病・疾患資源研究部, サブリーダー (20392300) ; KAWABATA Kenji 独立行政法人医薬基盤研究所, 創薬基盤研究部, プロジェクトリーダー (50356234)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2009: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 口腔解剖学 / 幹細胞研究 / 再生医学 / 細胞工学 / 幹細胞 / iPS細胞 / 発生 / 分化 / 再生 / 神経堤細胞 / 顎骨・歯胚

Research Abstract

Because Human iPS(hiPS) cells have the differentiation potential as like human ES cells, human iPS cells may be useful tool to induce maxilla-facial bone and tooth. However, it is well known that there is diversity in differentiating tendency of iPS cells. ES/iPS cell lines were chosen as tools of this experiment based on the analysis of gene expression profiles. Using chemically defined serum-free monolayer culture and multiwell-plate, differentiation of hiPS cells into endoderm and neural crest was examined. Further, neural crest marker expression was determined in the cells treated with inhibitors. However, regional gene expression was not detected in the cells. Further investigation is needed to induce cranial neural crest from hiPS cells.

Research Products

• [Journal Article] Efficient generation of functional hepatocytes from human embryonic stem cells and induced pluripotent stem cells by HNF4αtransduction (2012)
  • Author(s): Takayama K, Inamura M, Kawabata K, Katayama K, Higuchi M, Tashiro K, Nonaka A, Sakurai F, Hayakawa T, Furue MK, Mizuguchi H
  • Journal Title: Mol. Ther. Volume: 20 Pages: 127-37
• [Journal Article] Efficient Generation of Functional Hepatocytes From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells by HNF4α Transduction (2012)
  • Author(s): Takayama K, Inamura M, Kawabata K, Katayama K, Higuchi M, Tashiro K, Nonaka A, Sakurai F, Hayakawa T, Furue MK, Mizuguchi H
  • Journal Title: Molecular Therapy Volume: VOL.20 Issue: 1 Pages: 127-137
  • DOI: 10.1038/mt.2011.234
  • Peer Reviewed
• [Journal Article] マウスES細胞由来神経堤細胞の毒性評価系応用への可能性 (2012)
  • Author(s): 三村純代、相原祐子、木村直大、菅三佳、平田みつひ、林洋平、浅島誠、岡本哲治、二川浩樹、古江-楠田美保
  • Journal Title: Tiss.Cult.Res.Commun. Volume: VOL.30 Pages: 151-160
• [Journal Article] Growth Factor-Defined Culture Medium for Human Mesenchymal Stem Cells (2011)
  • Author(s): Mimura S, Kimura N, Hirata M, Tateyama D, Hayashida M, Umezawa A, Kohara A, Nikawa H, Okamoto T, Furue MK
  • Journal Title: Int J Dev Biol Volume: 55(2) Pages: 181-7
• [Journal Article] Efficient and selective generation of two distinct endoderm lineages from human ES and iPS cells by differentiation stage-specific SOX17 transduction (2011)
  • Author(s): Takayama K, Inamura M, Kawabata K, Tashiro K, Katayama K, Hayakawa T, Furue MK, Mizuguchi H
  • Journal Title: PLoS One Volume: 6
• [Journal Article] 日本におけるヒトES、iPS細胞研究標準化:その3品質管理 (2011)
  • Author(s): 平田みつひ、シャンダー・アハマド、菅三佳、藤木彩加、松村紘子、若林真理、上田直子、劉克紅、林田みどり、平山知子、小原有弘、柳原佳奈、水口賢司、古江-楠田美保
  • Journal Title: Tiss. Cult. Res. Commun Volume: 30 Pages: 137-149
  • NAID: 10030355386
• [Journal Article] マウスES細胞由来神経堤細胞の毒性評価系応用への可能性 (2011)
  • Author(s): 三村純代、相原祐子、木村直大、菅三佳、平田みつひ、林洋平、浅島誠、岡本哲治、二川浩樹、古江-楠田美保
  • Journal Title: Tiss. Cult. Res. Commun Volume: 30 Pages: 151-160
• [Journal Article] Efficient and Directive Generation of Two-Distinct Endoderm Lineages from Human ESCs and iPSCs by Differentiation Stage-Specific SOX17 Transduction (2011)
  • Author(s): Takayama K, Inamura M, Kawabata K, Tashiro K, Katayama K, Sakurai F, Hayakawa T, Furue MK, Mizuguchi H
  • Journal Title: PLoS One Volume: VOL.6 Issue: 2 Pages: 400-407
  • DOI: 10.1038/mt.2010.241
  • Peer Reviewed
• [Journal Article] Use of BAC array CGH for evaluation of chromosomal stability of clinically used human mesenchymal stem cells and of cancer cell lines (2011)
  • Author(s): Soichiro Saito, Keiko Morita, Arihiro Kohara, Tohru Masui, Mari Sasao, Hajime Ohgushi, Takashi Hirano
  • Journal Title: Human Cell Volume: VOL.24(1) Issue: 1 Pages: 2-8
  • DOI: 10.1007/s13577-010-0006-8
  • Peer Reviewed
• [Journal Article] Growth factor-defined culture medium for human mesenchymal stem cells (2011)
  • Author(s): 三村純代
  • Journal Title: The International Journal of Developmental Biology Volume: (記載確定)
  • Peer Reviewed
• [Journal Article] BMP4 induction of trophoblast from mouse embryonic stem cells in defined culture conditions on laminin (2010)
  • Author(s): Yohei Hayashi, Miho Kusuda Furue, Satoshi Tanaka, Michiko Hirose, Noriko Wakisaka Hiroki Danno, Kiyoshi Ohnuma, Shiho Oeda, Yuko Aihara, Kunio Shiota, Atsuo Ogura, Shoichi Ishiura, and Makoto Asashima
  • Journal Title: In Vitro Cellular & Devopmental Biology Animal Volume: 23 Pages: 114-22
• [Journal Article] Efficient generation of hepatoblasts from human ES cells and iPS cells by transient overexpression of homeobox gene HEX (2010)
  • Author(s): Inamura M, Kawabata K, Takayama K, Tashiro K, Sakurai F, Katayama K, Toyoda M, Akutsu H, Miyagawa Y, Okita H, Kiyokawa N, Umezawa A, Hayakawa T, Furue MK, Mizuguchi H
  • Journal Title: Molecular Therapy Volume: 19(2) Pages: 400-7
• [Journal Article] Reduction of N-glycolylneuraminic Acid in Human in Induced Pluripotent Stem Cells Generated or cultured under Feeder-and Serum-free Defined Conditions (2010)
  • Author(s): Hayashi Y, Chan T, Warashina M, Fukuda M, Ariizumi T, Okabayashi K, Takayama N, Otsu M, Eto K, Furue MK, Michiue T, Ohnuma K, Nakauchi H, Asashima M
  • Journal Title: Plos One Volume: 5(11)
• [Journal Article] Adenovirus vector-mediated efficient transduction into human embryonic and induced pluripotent stem cells (2010)
  • Author(s): Tashiro K., Kawabata K., Inamura M., Takayama K., Furukawa N., Sakurai F., Katayama K., Hayakawa H., Furue-Kusuda M., Mizuguchi H.
  • Journal Title: Cell Reprogram Volume: 12(5) Pages: 501-7
• [Journal Article] Induction of neural crest cells from mouse embryonic stem cells in a serum-free monolayer culture (2010)
  • Author(s): Aihara Y, Hayashi Y, Hirata M, Ariki N, Shibata S, Nagoshi N, Nakanishi M, OhnumaK, Warashina M, Michiue T, Uchiyama H, Okano H, Asashima M, Furue MK
  • Journal Title: The international Journal of Developmental Biology Volume: 54 Pages: 1287-129
• [Journal Article] Inhibition of ERK1/2 Prevents Neural and Mesendodermal Differentiation and Promotes Human Embryonic Stem Cell Self-renewal (2010)
  • Author(s): Na J, Furue MK, Andrews PW
  • Journal Title: Stem Cell Research Volume: 5(2) Pages: 157-69
• [Journal Article] Advantages and difficulties in culturing human pluripotent stem cells in growth factor-defined serum-free medium (2010)
  • Author(s): Furue MK, Tateyama D, Kinehara M, Na J, Okamoto T, Sato JD
  • Journal Title: Vitro Cellular & Developmental Biology. Animal Volume: 46 Pages: 573-576
• [Journal Article] Advantages and difficulties in culturing human pluripotent stem cells in growth factor-defined serum-free medium (2010)
  • Author(s): 古江-楠田美保
  • Journal Title: In Vitro Cellular & Developmental Biology Animal. Volume: 46 Pages: 573-576
  • Peer Reviewed
• [Journal Article] Induction of neural crest cells from mouse embryonic stem cells in a serum-free monolaver culture (2010)
  • Author(s): 相原祐子
  • Journal Title: The International Journal of Developmental Biology Volume: 54 Pages: 1287-1294
  • Peer Reviewed
• [Journal Article] BMP4 Induces Trophoblast from Mouse Embryonic Stem Cells through the activation of Cdx2 expression. (2010)
  • Author(s): 林洋平
  • Journal Title: In Vitro Cellular & Devopmental Biology Animal. (記載確定)
  • Peer Reviewed
• [Journal Article] BMP4 Induces Trophoblast from Mouse Embryonic Stem Cells in defined culture conditions on laminin. (2010)
  • Author(s): 林洋平
  • Journal Title: In Vitro Cellular & Devopmental Biology Animal. 46 Pages: 416-430
  • Peer Reviewed
• [Journal Article] 日本におけるヒトES、iPS細胞研究標準化:その2分化能の評価 (2009)
  • Author(s): 古江-楠田美保
  • Journal Title: Tissu Culture Research Communications Volume: 28 Pages: 129-133
• [Journal Article] Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium (2009)
  • Author(s): 中西未央
  • Journal Title: FASEB J. 23 Pages: 114-122
  • Peer Reviewed
• [Journal Article] 日本におけるヒトES、ips細胞研究標準化:その2 分化能の評価 (2009)
  • Author(s): 古江-楠田美保
  • Journal Title: Tissue culture research communications 28 Pages: 129-133
• [Presentation] FOXA2・HNF1・遺伝子導入によるヒト多能性幹細胞から薬剤代謝能を有した肝細胞の分化誘導 (2012)
  • Author(s): 高山和雄、稲村充、川端健二、菅原道子、菊池きよ美、櫻井文教、古江-楠田美保、水口裕之
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] 3次元共培養法によるヒトES・iPS細胞由来肝細胞の効率的な分化誘導法の開発 (2012)
  • Author(s): 長基康人、田代克久、高山和雄、大橋一夫、櫻井文教、立花雅史、古江-楠田美保、川端健二、水口裕之
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] 多能性幹細胞からの歯再生の可能性 (2011)
  • Author(s): 古江-楠田美保
  • Organizer: 第9回再生歯科医学会学術大会・総会
  • Place of Presentation: 大阪
  • Year and Date: 2011-09-10
• [Presentation] 多能性幹細胞からの歯再生の可能性 (2011)
  • Author(s): 古江-楠田美保
  • Organizer: 第9回再生歯科医学会学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-09-10
• [Presentation] 創薬応用のためのヒト多能性幹細胞の品質 (2011)
  • Author(s): 古江-楠田美保
  • Organizer: PhI Japan 2011大阪国際医薬品原料・中間体展
  • Place of Presentation: 大阪
  • Year and Date: 2011-07-15
• [Presentation] NF4α遺伝子導入によるヒトES・ヒトiPS細胞からの成熟肝細胞への高効率分化誘導 (2011)
  • Author(s): 高山和雄、稲村充、川端健二、形山和史、櫻井文教、古江-楠田美保、水口裕之
  • Organizer: 第131年会日本薬学会
  • Place of Presentation: 静岡
• [Presentation] ヒト胚性幹細胞、人工多能性幹細胞、間葉系幹細胞の細胞特性比較解析 (2011)
  • Author(s): 平田みつひ、林田みどり、館山大揮、小澤裕、松村紘子、家村将士、正札智子、金村米博、小原有弘、川端健二、水口賢司、水口裕之、古江-楠田美保
  • Organizer: 日本組織培養学会第84回大会
  • Place of Presentation: 東京
• [Presentation] ヒト間葉系幹細胞の未分化性維持を目的とした既知因子のみで構成される無血清培養法 (2011)
  • Author(s): 三村純代、木村直大、平田みつひ、舘山大輝、林田みどり、小原有弘、岡本哲治、二川浩樹、古江-楠田美保
  • Organizer: 第59回国際歯科研究学会日本部会総会・学術大会
  • Place of Presentation: 広島
• [Presentation] EMT-related gene expression during cell differentiation into extraembryonic endoderm in human embryonic stem cells (2011)
  • Author(s): Kawamura S., Kinehara M., Nikawa H., Furue MK
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Establishment of an efficient neural crest cell induction system from mouse embryonic stem cells in growth factor defined serum-free medium (2011)
  • Author(s): Aihara. Y., Hayashi. Y., Hirata. M., Ariki. N., Shibata. S., Nagoshi. N., Nakanishi. M., Ohnuma. K., Warashina M., Michiue T., Uchiyama H., Okano H., Asashima M., Furue, MK
  • Organizer: International Society for Stem Cell Research(ISSCR 9th Annual Meeting)
  • Place of Presentation: トロント
• [Presentation] Generation of Human Induced Pluripotent Stem Cells from Adult Dermal Fibroblast under Feeder-and Serum-free Defined Conditions (2011)
  • Author(s): Hayashi Y., Chan T., Warashina M., Fukuda M., Ariizumi T., Okabayashi K., Takayama N., Otsu M., Eto K., Furue MK., Michiue T., Ohnuma K., Nakauchi H., Asashima M
  • Organizer: International Society for Stem Cell Research(ISSCR 9th Annual Meeting)
  • Place of Presentation: トロント
• [Presentation] Protein kinase C induces epithelial-mesenchymal transition in human ES and iPS cells (2011)
  • Author(s): Kinehara M., Kawamura S., Tateyama D., Matsumura H., Hirata M., Furue MK
  • Organizer: International Society for Stem Cell Research(ISSCR 9th Annual Meeting)
  • Place of Presentation: トロント
• [Presentation] Comparative analysis of characteristics among human iPS, ES and neuroblastoma cell lines (2011)
  • Author(s): Hirata M., Hayashida M., Tateyama D., Ozawa Y., Matsumura H., Iemura M., Shofuda T., Kanemura Y., Kohara A., Kawabata K., Mizuguchi H., Furue MK
  • Organizer: International Society for Stem Cell Research(ISSCR 9th Annual Meeting)
  • Place of Presentation: トロント
• [Presentation] Development of a novel drug screening system using human iPS cells in a defined culture system. Rediscovering pluripotency : From teratocarcinomas to embryonic stem cells (2011)
  • Author(s): Furue, MK
  • Place of Presentation: イギリスカーディフ
• [Presentation] NF4・遺伝子導入によるヒトES・iPS細胞からの成熟肝細胞への高効率分化誘導 (2011)
  • Author(s): 高山和雄、稲村充、川端健二、田代克久、形山和史、櫻井文教、古江-楠田美保、水口裕之
  • Organizer: 第18回肝細胞研究会
  • Place of Presentation: 東京
• [Presentation] Chemically Defined Serum-Free Medium for Undifferentiated Human Mesenchymal Stem Cells (2011)
  • Author(s): 三村純代、木村直大、平田みつひ、舘山大輝、林田みどり、小原有弘、岡本哲治、二川浩樹、古江-楠田美保
  • Organizer: 第59回国際歯科研究学会日本部会総会・学術大会
  • Place of Presentation: 広島
• [Presentation] EFFICIENT GENERATION OF MATURE HEPATOCYTES FROM HUMAN PLURIPOTENT STEM CELLS BY HNF4α TRANSDUCTION (2011)
  • Author(s): Takayama K., Inamura M., Kawabata K., Katayama K., Tashiro K., Sakurai F., Furue MK., Mizuguchi H.
  • Organizer: 第26回日本薬物動態学会年会
  • Place of Presentation: 広島(ベストポスター賞受賞)
• [Presentation] Establishment of an efficient neural crest cell induction system from mouse embryonic stem cells in growth factor defined serum-free medium (2011)
  • Author(s): Aihara Y., Hayashi Y., Hirata M., Ariki N., Shibata S., Nagoshi N., Nakanishi M., Ohnuma K., Warashina M., Michiue T., Uchiyama H., Okano H., Asashima M., Furue, MK.
  • Organizer: International Society for Stem Cell Research (ISSCR 9th Annual Meeting)
  • Place of Presentation: トロント
• [Presentation] EFFICIENT GENERATION OF FUNCTIONAL HEPATOCYTES FROM HUMAN EMBRYONIC STEM CELLS AND INDUCED PLURIPOTENT STEM CELLS BY HNF4α TRANSDUCTION (2011)
  • Author(s): Takayama K., Inamura M., Kawabata K., Katayama K., Tashiro K., Sakurai F., Furue MK., Mizuguchi H.
  • Organizer: International Society for Stem Cell Research (ISSCR 9th Annual Meeting)
  • Place of Presentation: トロント
• [Presentation] Generation of Human Induced Pluripotent Stem Cells from Adult Dermal Fibroblast under Feeder- and Serum-free Defined Conditions (2011)
  • Author(s): Hayashi Y., Chan T., Warashina M., Fukuda M., Ariizumi T., Okabayashi K., Takayama N., Otsu M., Eto K., Furue MK., Michiue T., Ohnuma K.(発表者), Nakauchi H., Asashima M.
  • Organizer: International Society for Stem Cell Research (ISSCR 9th Annual Meeting)
  • Place of Presentation: トロント
• [Presentation] Protein kinase C induces epithelial-mesenchymal transition in human ES and iPS cells (2011)
  • Author(s): Kinehara M., Kawamura S., Tateyama D., Matsumura H., Hirata M., Furue MK.
  • Organizer: International Society for Stem Cell Research (ISSCR 9th Annual Meeting)
  • Place of Presentation: トロント
• [Presentation] DEVELOPMENT OF A DEFINED SERUM FREE AND XENO FREE MEDIUM COMPOSED OF MINIMAL COMPONENTS FOR CULTURING HUMAN ES/IPS CELLS (2011)
  • Author(s): Tateyama D., Kinehara M., Hirata M., Matsumura H., Inoue S., Furue MK.
  • Organizer: International Society for Stem Cell Research (ISSCR 9th Annual Meeting)
  • Place of Presentation: トロント
• [Presentation] Comparative analysis of characteristics among human iPS, ES and neuroblastoma cell lines (2011)
  • Author(s): Hirata M., Hayashida M., Tateyama D., Ozawa Y., Matsumura H., Iemura M., Shofuda T., Kanemura Y., Kohara A., Kawabata K., Mizuguchi H., Furue MK.
  • Organizer: International Society for Stem Cell Research (ISSCR 9th Annual Meeting)
  • Place of Presentation: トロント
• [Presentation] HNF4α promotes hepatic maturation from human embryonic stem cell-derived hepatoblasts (2011)
  • Author(s): Takayama K., Inamura M., Kawabata K., Katayama K., Tashiro K., Sakurai F., Furue MK., Mizuguchi H.
  • Organizer: The 6th Seoul-Kyoto-Osaka Joint Symposium on Pharmaceutical Sciences for Young Scientists
  • Place of Presentation: ソウル
• [Presentation] Development of a novel drug screening system using human iPS cells in a defined culture system (2011)
  • Author(s): Furue, MK.
  • Organizer: Rediscovering pluripotency : From teratocarcinomas to embryonic stem cells
  • Place of Presentation: イギリスカーディフ
• [Presentation] ヒトES/iPS細胞の評価法開発 (2010)
  • Author(s): 古江-楠田美保
  • Organizer: 埼玉医科大学ゲノム医学研究センター運営委員会承認学術集会
  • Place of Presentation: 埼玉医科大学ゲノム医学研究センター(埼玉県)
  • Year and Date: 2010-10-25
• [Presentation] Development of a drug toxicity test using human TS cells and serum-free culture conditions (2010)
  • Author(s): 古江-楠田美保
  • Organizer: 第23回日本動物細胞工学会2010年度国際会議シンポジウム発表
  • Place of Presentation: 北海道大学札幌キャンパスConference Hall(北海道)
  • Year and Date: 2010-09-03
• [Presentation] Development of an alkaline phosphatase activity-based high throughput screening assay using human iPS cells with chemically defined culture conditions (2010)
  • Author(s): 木根原匡希
  • Organizer: 日本組織培養学会第83回大会一般口演
  • Place of Presentation: 岡山大学創立五十周年記念館ホール(岡山県)
  • Year and Date: 2010-05-21
• [Presentation] TGF-β1は未分化性と多分化能を維持したヒト間葉系幹細胞増殖を促進する (2010)
  • Author(s): 三村純代
  • Organizer: 日本組織培養学会第83回大会一般口演
  • Place of Presentation: 岡山大学創立五十周年記念館ホール(岡山県)
  • Year and Date: 2010-05-20
• [Presentation] TGF-β1は未分化性と多分化能を維持したヒト間葉系幹細胞増殖を促進する (2010)
  • Author(s): 三村純代、木村直大、平田みつひ、舘山大輝、林田みどり、小原有弘、岡本哲治、二川浩樹、古江-楠田美保
  • Organizer: 日本組織培養学会第83回大会
  • Place of Presentation: 岡山
• [Presentation] HEX遺伝子の導入によるヒトES細胞やヒトiPS細胞からの効率良い肝幹細胞への分化誘導 (2010)
  • Author(s): 稲村充、川端健二、高山和雄、田代克久、形山和史、櫻井文教、古江-楠田美保、水口裕
  • Organizer: 第16回肝細胞研究会
  • Place of Presentation: 秋田
• [Presentation] マウス人工多能性幹(iPS)細胞の未分化性と多分化能を維持した単層無血清培養系の確立 (2010)
  • Author(s): 鍋島巧、山崎佐知子、木村直太、古江-楠田美保、岡本哲治
  • Organizer: 第64回日本口腔科学会学術集会
  • Place of Presentation: 北海道
• [Presentation] SOX17遺伝子導入によるヒトES・ヒトiPS細胞からの内胚葉および胚体外内胚葉への選択的分化誘導 (2010)
  • Author(s): 高山和雄、稲村充、田代克久、形山和史、櫻井文教、古江-楠田美保、川端健二、水口裕之
  • Organizer: 第17回肝細胞研究会
  • Place of Presentation: 秋田
• [Presentation] Comparison analysis of characteristics among human iPS, ES and neuroblastoma cell lines (2010)
  • Author(s): Hirata, M., Hayashida, M., Tateyama, D., Ozawa, Y., Matsumura, H., Iemura, M., Kohara, A., Furue, MK
  • Organizer: 第8回ISSCR
  • Place of Presentation: サンフランシスコ
• [Presentation] マウス人工多能性幹(iPS)細胞の無血清培養系の確立 (2010)
  • Author(s): 山崎佐知子、古江-楠田美保、岡本哲治
  • Organizer: 第47回日本口腔組織培養学会学術大会
  • Place of Presentation: 高知
• [Presentation] DEVELOPMENT OF AN ALKALINE PHOSPHATASE ACTIVITY-BASED HIGH-THROUGHPUT SCREENING ASSAY USING HUMAN IPS CELLS WITH CHEMICALLY DEFINED CULTURE CONDITIONS (2010)
  • Author(s): 木根原匡希
  • Organizer: ISSCR国際幹細胞学会8th Amlual Meetingポスター発表
  • Place of Presentation: Moacone Weat(カリフォルニアU.S.A)
• [Presentation] COMPARISON ANALYSIS OF CHARACTERISTICS AMONG HUMAN IPS, ES AND NEUROBLASTOMA CELL LINES (2010)
  • Author(s): 平田みつひ
  • Organizer: ISSCR国際幹細胞学会8thAnnual Meetingポスター発表
  • Place of Presentation: Moacone Weat(カリフォルニアU.S.A)
• [Presentation] ヒトES細胞ならびにiPS細胞の細胞表面抗原発現による標準化 (2009)
  • Author(s): 古江-楠田美保
  • Organizer: 第32回日本分子生物学会 ワークショップ幹細胞と糖鎖
  • Place of Presentation: 神奈川県 パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] Adenovirus vector-mediated efficient transduction into humaninduced pluripotent stem cells. (2009)
  • Author(s): 田代克久
  • Organizer: 第32回日本分子生物学会 ポスター発表
  • Place of Presentation: 神奈川県 パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] ヒトES、ips細胞における創薬応用のための標準化 (2009)
  • Author(s): 古江-楠田美保
  • Organizer: 第22回日本動物実験代替法学会総会
  • Place of Presentation: 大阪大学 銀否会館
  • Year and Date: 2009-11-14
• [Presentation] 未分化ヒトES細胞から中内胚葉へのラミニンによる分化促進効果 (2009)
  • Author(s): 稲村充
  • Organizer: フオーマ・バイオフォーラム2009
  • Place of Presentation: 愛知県 名古屋市立大学田辺通キャンパス
  • Year and Date: 2009-11-14
• [Presentation] ヒトES細胞やiPS細胞からの内胚葉系細胞および肝細胞への分化誘導法の開発 (2009)
  • Author(s): 稲村充
  • Organizer: 第16回肝細胞研究会
  • Place of Presentation: 山形県 山形テルサ
  • Year and Date: 2009-06-26
• [Presentation] Integrins expression profile in human ES and iPS cells in the defined culture conditions (2009)
  • Author(s): 舘山大揮
  • Organizer: 第7回 ISSCR
  • Place of Presentation: スペイン バルセロナ
  • Year and Date: 2009-06-10
• [Presentation] LAMININ PROMOTES HUMAN EMBRYONIC STEM CELL DIFFERENTIATION INTO MESODENDODERM (2009)
  • Author(s): 稲村充
  • Organizer: 第7回 ISSCR
  • Place of Presentation: スペイン バルセロナ
  • Year and Date: 2009-06-10
• [Presentation] 創薬応用のためのヒトES、ips細胞の標準化 (2009)
  • Author(s): 古江-楠田美保
  • Organizer: 日本組織培養学会第82回大会シンポジウム(III)創薬
  • Place of Presentation: 栃木県 独協医科大学創立30周年記念館
  • Year and Date: 2009-05-19
• [Presentation] 無血清培養下におけるヒト胚性幹細胞ならびに人工多能性幹細胞のインテグリン発現プロファイル (2009)
  • Author(s): 舘山大揮
  • Organizer: 日本組織培養学会第82回大会 一般口演
  • Place of Presentation: 栃木県 独協医科大学創立30周年記念館
  • Year and Date: 2009-05-18
• [Presentation] 未分化ヒトES細胞から中内胚葉へのラミニンによる分化促進効果 (2009)
  • Author(s): 稲村充
  • Organizer: 日本組織培養学会第82回大会 一般口演
  • Place of Presentation: 栃木県 独協医科大学創立30周年記念館
  • Year and Date: 2009-05-18
• [Presentation] マウスES細胞から誘導された神経堤細胞の特性解析 (2009)
  • Author(s): 相原祐子
  • Organizer: 日本組織培養学会第82回大会 一般口演
  • Place of Presentation: 栃木県 独協医科大学創立30周年記念館
  • Year and Date: 2009-05-18
• [Presentation] Integrins expression profile in human ES and iPS cells in the defined culture conditions (2009)
  • Author(s): Daiki Tateyama, Naohiro Kimura, Midori Hyashida, Yutaka Ozawa, Hiroko atsumura, Arihiro Kohara, Tetsuji Okamoto, Akihiro Umesawa, and Miho Kusuda Furue
  • Organizer: 第7回ISSCR
  • Place of Presentation: スペインバルセロナ
• [Presentation] LAMININ PROMOTES HUMAN EMBRYONIC STEM CELL DIFFERENTIATION INTO MESODENDODERM (2009)
  • Author(s): Inamura, Mitsuru, Kawabata, Kenji, Sakurai, Fuminori, Katayama, Kazufumi, Hayashida, Midori, Matsumura, Hiroko, Furue, Miho Kusuda, Mizuguchi, Hiroyuki
  • Organizer: 第7回ISSCR
  • Place of Presentation: スペインバルセロナ
• [Presentation] ヒトES、iPS細胞における創薬応用のための標準化 (2009)
  • Author(s): 古江-楠田美保
  • Organizer: ワークショップ第22回日本動物実験代替法学会総会
  • Place of Presentation: 大阪
• [Presentation] ヒトES細胞ならびにiPS細胞の細胞表面抗原発現による標準化Standardization of human ES and iPS cells by analyzing cell surface antigens (2009)
  • Author(s): 古江-楠田美保
  • Organizer: 第32回日本分子生物学会ワークショップ幹細胞と糖鎖
  • Place of Presentation: 横浜
• [Presentation] 無血清培養下におけるヒト胚性幹細胞ならびに人工多能性幹細胞のインテグリン発現プロファイル (2009)
  • Author(s): 舘山大揮,木村直大,林田みどり,小澤裕,松村紘子,小原有弘, Paul J Gokhale,岡本哲治,梅澤明弘, Peter W. Andrews,古江-楠田美保
  • Organizer: 日本組織培養学会第82回大会(一般口演)
  • Place of Presentation: 茨木
• [Presentation] 未分化ヒトES細胞から中内胚葉へのラミニンによる分化促進効果 (2009)
  • Author(s): Mitsuru Inamura, Kenji Kawabata, Fuminori Sakurai, Kazufumi Katayama, Midori Hayashida, Hiroko Matsumura, Miho Kusuda Furue, Hiroyuki Mizuguchi
  • Organizer: 日本組織培養学会第82回大会(一般口演)
  • Place of Presentation: 茨木
• [Presentation] Adenovirus vector-mediated efficient transduction into human induced pluripotent stem cells (2009)
  • Author(s): Katsuhisa Tashiro, Mitsuru Inamura, Norihisa Furukawa, Kenji Kawabata, Fuminori Sakurai, Miho Kusuda Furue, Hiroyuki Mizuguchi
  • Organizer: 第32回日本分子生物学会(一般口演)
  • Place of Presentation: 横浜
• [Presentation] ヒトES細胞やiPS細胞からの内胚葉系細胞および肝細胞への分化誘導法の開発 (2009)
  • Author(s): 稲村充、川端健二、形山和史、梅澤明弘、阿久津英憲、林田みどり、松村紘子、古江-楠田美保、水口裕之
  • Organizer: 第16回肝細胞研究会
  • Place of Presentation: 山形
• [Presentation] 未分化ヒトES細胞から中内胚葉へのラミニンによる分化促進効果 (2009)
  • Author(s): 稲村充、川端健二、形山和史、林田みどり、松村紘子、古江-楠田美保、水口裕之
  • Organizer: フォーマ・バイオフォーラム
  • Place of Presentation: 名古屋
• [Presentation] 創薬応用のためのヒトES、iPS細胞の標準化
  • Author(s): 古江-楠田美保
  • Organizer: 日本組織培養学会第82回大会シンポジウム(III)創薬
  • Place of Presentation: 茨木
• [Book] In : STEM CELLS AND CANCER STEM CELLS, Volume 5 : Therapeutic Applications in Disease and Injury. M. A. Hayat Ed. Monolayer culture condition for Mouse Embryonic Stem Cells differentiation into Neural Crest Cells (2012)
  • Author(s): Miho Kusuda Furue
  • Publisher: Springer, Amsterdam
• [Book] iPS細胞を活用した安全性・有効性評価系の構築、iPS細胞の産業的応用技術 (2009)
  • Author(s): 古江-楠田美保、山田弘、水口裕之
  • Publisher: シーエムシー出版
• [Book] 遺伝子医学MOOK別冊 (2009)
  • Author(s): 古江-楠田美保
  • Publisher: メディカルドゥ
• [Book] iPS細胞を活用した安全性・有効性評価系の構築、iPS細胞の産業的応用技術 (2009)
  • Author(s): 古江-楠田美保
  • Total Pages: 7
  • Publisher: シーエムシー出版
• [Book] 第5章細胞周辺環境のための培養技術 1培養液,ますます重要になる細胞周辺環境(細胞ニッチ)の最新科学技術」-細胞の生存、増殖、機能のコントロールから創薬研究、再生医療まで-遺伝子医学MOOK別冊 (2009)
  • Author(s): 古江-楠田美保
  • Total Pages: 4
  • Publisher: メディカルドゥ
• [Remarks]
  • URL: http://www.nibio.go.jp/baiyou/index.html
• [Remarks]
  • URL: http://www.nbio.go.jp/part/disease/culture_resources/
• [Remarks]
  • URL: http://www.nibio.go.jp/nanbyoushigen/baiyou.html
• [Patent(Industrial Property Rights)] 幹細胞から肝細胞への分化誘導方法 (2009)
  • Inventor(s): 水口裕之
  • Industrial Property Rights Holder: 財団法人ヒューマンサイエンス振興財団
  • Industrial Property Number: 2009-247342
  • Filing Date: 2009-10-28