| Project/Area Number |
21390493
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Morphological basic dentistry
|
|---|
| Research Institution | National Institute of Biomedical Innovation |
|---|
Principal Investigator |
FURUE Miho 独立行政法人医薬基盤研究所, 難病・疾患資源研究部, 研究リーダー (80257310)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KOHARA Arihiro 独立行政法人医薬基盤研究所, 難病・疾患資源研究部, サブリーダー (20392300)
KAWABATA Kenji 独立行政法人医薬基盤研究所, 創薬基盤研究部, プロジェクトリーダー (50356234)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
|
|---|
| Keywords | 口腔解剖学 / 幹細胞研究 / 再生医学 / 細胞工学 / 幹細胞 / iPS細胞 / 発生 / 分化 / 再生 / 神経堤細胞 / 顎骨・歯胚 |
|---|
| Research Abstract |
Because Human iPS(hiPS) cells have the differentiation potential as like human ES cells, human iPS cells may be useful tool to induce maxilla-facial bone and tooth. However, it is well known that there is diversity in differentiating tendency of iPS cells. ES/iPS cell lines were chosen as tools of this experiment based on the analysis of gene expression profiles. Using chemically defined serum-free monolayer culture and multiwell-plate, differentiation of hiPS cells into endoderm and neural crest was examined. Further, neural crest marker expression was determined in the cells treated with inhibitors. However, regional gene expression was not detected in the cells. Further investigation is needed to induce cranial neural crest from hiPS cells.
|
