Development of control system for pathogenic oral biofilm using compound and new proteins

• Project/Area Number: 21390506
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: SENPUKU Hidenobu 国立感染症研究所, 細菌第一部, 室長 (20250186)
• Co-Investigator(Kenkyū-buntansha): KAWARAI Taketo 国立感染症研究所, 細菌第一部, 研究員 (90409079) ; NAKAO Ryoma 国立感染症研究所, 細菌第一部, 主任研究官 (10370959) ; SUGANO Naoyuki 日本大学, 歯学部, 准教授 (30246904)
• Co-Investigator(Renkei-kenkyūsha): WATANABE Haruo 国立感染症研究所, 所長 (70142130)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
• Keywords: バイオフィルム / 抑制物質 / ペプチド / 唾液分泌低下 / う蝕予防 / Streptococcus mutans / Streptococcus gordonii / NOD/SCID, E2f-1^<-/-> / IgA / フルクタナーゼ / NOD/SCID,E2f-1 / NOD / SCID, E2f-1^<- / ->

Research Abstract

We investigated development of preventive methods for oral diseases using new drug. Polypyrroles and fructanase inhibited biofilm formation, which cause dental caries and periodontal diseases. This inhibition effects were confirmed in the new animal model ; NOD/SCID, E2f-1^<-/-> mice. From these results, it was considered that polypyrroles and fructanase were useful for development of prevention system for dental diseases.

Research Products

• [Journal Article] Roles of salivary components in Streptococcus mutans colonization in a new animal model using NOD/SCID. e2f1^-mice (2012)
  • Author(s): Ito T, Maeda T, Senpuku H
  • Journal Title: PLoS ONE Volume: 7 Issue: 2 Pages: e32063-e32063
  • DOI: 10.1371/journal.pone.0032063
  • Peer Reviewed
• [Journal Article] Streptococcus salivarius FruA Inhibits Streptococcus mutans biofilm formation (2011)
  • Author(s): Ogawa A, Furukawa S, Fujita S, Mitobe J, Kawarai T, Narisawa N, Sekizuka T, Kuroda M, Ochiai K, Hirokazu Ogihara H, Kosono S, Yoneda S, Watanabe H, Morinaga Y, Uematsu H, and Senpuku H
  • Journal Title: Applied Environmental Microbiology Volume: 77 Issue: 5 Pages: 1572-1580
  • DOI: 10.1128/aem.02066-10
• [Journal Article] Competence-dependent endogenous DNA rearrangement and uptake of extracellular DNA gives a natural variant of Streptococcus mutans without biofilm formation (2011)
  • Author(s): Narisawa N, Kawarai T, Suzuki N, Sato Y, Ochiai K, Ohnishi M, Watanabe H, and Senpuku H
  • Journal Title: Journal of Bacteriology Volume: 193 Issue: 19 Pages: 5147-5154
  • DOI: 10.1128/jb.05240-11
• [Journal Article] Outer membrane vesicles of Porphyromonas gingivalis elicit a mucosal immune response (2011)
  • Author(s): Nakao R, Hasegawa H, Ochiai K, Takashiba S, Ainai A, Ohnishi M, Watanabe H, and Senpuku H
  • Journal Title: PloS ONE Volume: 6 Issue: 10 Pages: e26163-e26163
  • DOI: 10.1371/journal.pone.0026163
  • Peer Reviewed
• [Journal Article] Hinokitiol inhibits Candida albicans adherence to oral epithelial cells (2010)
  • Author(s): Nakamura M, Fujibayashi T, Tominaga A, Satoh N, Kawarai T, Shinozuka O, Watanabe H, Yamazaki T, Senpuku H
  • Journal Title: Journal of Oral Biosciences Volume: 52 Pages: 42-50
  • NAID: 10027105301
• [Journal Article] Effects of mucosal care on oral pathogens in professional oral hygiene to the elderly (2010)
  • Author(s): Nishiyama Y, Inaba E, Uematsu H, Senpuku H
  • Journal Title: Archives of Gerontology & Geriatrics Volume: 51
  • URL: http://dx.doi.org/10.1016/j.archger.2010.04.009
• [Journal Article] A quick statistically accurate diagnosis for caries risk in the elderly (2010)
  • Author(s): Senpuku H, Miyazaki H, Yoneda S, Yoshihara A, Tada A
  • Journal Title: Clinical Laboratory Volume: 56 Pages: 505-512
• [Journal Article] Inhibition of Streptococcus mutans adherence and biofilm formation using analogues of the SspB peptide (2010)
  • Author(s): Okuda K, Hanada N, Usui Y, Takeuchi H, Koba H, Nakao R, Watanabe H, and Senpuku H
  • Journal Title: Archives of Oral Biology Volume: 55 Pages: 754-762
  • URL: http://dx.doi.org/10.1016/j.archoralbio.2010.06.014
• [Journal Article] Effects of mucosal care on oral pathogens in professional oral hygiene to the elderly (2010)
  • Author(s): Yoshihide Nishiyama, et al.
  • Journal Title: Archives of Gerontology & Geriatrics Volume: 51
  • Peer Reviewed
• [Journal Article] A quick statistically accurate diagnosis for caries risk in the elderly. (2010)
  • Author(s): Hidenobu Senpuku, et al.
  • Journal Title: Clinical Laboratory Volume: 56 Pages: 505-512
  • Peer Reviewed
• [Journal Article] Inhibition of Streptococcus mutans adherence and biofilm formation using analogues of the SspB peptide. (2010)
  • Author(s): Kentaro Okuda, et al.
  • Journal Title: Archives of Oral Biology Volume: 55 Pages: 754-762
  • Peer Reviewed
• [Journal Article] Hinokitiol inhibits Candida albicans adherence to oral epithelial cells (2010)
  • Author(s): Moriyuki Nakamura, et al.
  • Journal Title: Journal of Oral Biosciences 52 Pages: 42-50
  • Peer Reviewed
• [Journal Article] E2F-1-deficient NOD/SCID mice developed showing decreased saliva production (2009)
  • Author(s): Matsui-Inohara H, Uematsu H, Narita T, Satoh K, Yonezawa H, Kuroda K, Ito T, Yoneda S, Kawarai T, Sugiya H, Watanabe H, Senpuku H
  • Journal Title: Experimental Biology and Medicine Volume: 234 Issue: 12 Pages: 1525-1536
  • DOI: 10.3181/0905-rm-173
• [Journal Article] Effects of IgY against Candida albicans and Candida spp. adherence and biofilm formation (2009)
  • Author(s): Fujibayashi T, Nakamura M, Tominaga A, Satoh N, Kawarai T, Narisawa N, Shinozuka O, Watanabe H, Yamazaki T, Senpuku H
  • Journal Title: Japanese Journal Infectious Diseases Volume: 62 Pages: 337-342
  • URL: http://www0.nih.go.jp/JJID/62/337.html
• [Journal Article] E2F-1-deficient NOD/SCID mice developed showing decreased saliva production (2009)
  • Author(s): Hikaru Matsui-Inohara, et al.
  • Journal Title: Experimental Biology and Medicine 234 Pages: 1525-1536
  • Peer Reviewed
• [Journal Article] Effects of IgY against Candida albicans and Candida spp.adherence and biofilm formation (2009)
  • Author(s): Taisuke Fujibayashi, et al.
  • Journal Title: Japanese Journal Infectious Diseases 62 Pages: 377-342
  • Peer Reviewed
• [Presentation] Effects of salivary components on Streptococcus mutans biofilm in NOD/SCID.e2f1^<-l-> mice (2012)
  • Author(s): 泉福英信、伊藤龍朗、渡邉治雄、大西真
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎、長崎ブリックホール
  • Year and Date: 2012-03-28
• [Presentation] Effects of salivary components on Streptococcus mutans biofilm in NOD/SCID. e2f1^<-/-> mice (2012)
  • Author(s): 泉福英信、伊藤龍朗、渡邉治雄、大西真
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] 口腔ケアによる口腔微生物バリアー構築と免疫活性化の可能性 (2011)
  • Author(s): 泉福英信
  • Organizer: 第20回日本口腔感染症学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-11-13
• [Presentation] 口腔ケアによる口腔微生物バリアー構築と免疫活性化の可能性 (2011)
  • Author(s): 泉福英信
  • Organizer: 第20回日本口腔感染症学会
  • Place of Presentation: 横浜、鶴見大学(招待講演)
  • Year and Date: 2011-11-13
• [Presentation] Streptococcus mutansにおけるCSP依存新規病原性関連遺伝子の検討 (2011)
  • Author(s): 鈴木奈央未、成澤直規、落合邦康、泉福英信
  • Organizer: 第53回歯科基礎医学会
  • Place of Presentation: 岐阜、長良川国際会議場
  • Year and Date: 2011-10-01
• [Presentation] FruA and SspB peptide from oral streptococci and saliva IgA to PAc(361-389) peptide play multiple functions in biofilm formation (2011)
  • Author(s): Senpuku H
  • Organizer: Aarhus University, Special Seminar
  • Place of Presentation: Aarhus, Denmark, USA
  • Year and Date: 2011-07-04
• [Presentation] FruA and SspB peptide from oral streptococci and saliva IgA to PAc (361-389) peptide play multiple functions in biofilm formation (2011)
  • Author(s): Senpuku H
  • Organizer: Aarhus University, Special Seminar
  • Place of Presentation: Aarhus, Denmark(招待講演)
  • Year and Date: 2011-07-04
• [Presentation] Diagnosis and prevension for dental caries development, cell to cell communication in dental biofilm formation (2011)
  • Author(s): Senpuku H
  • Organizer: Istanbul University Dental school, Special Seminar
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2011-07-01
• [Presentation] Diagnosis and prevension for dental caries development, cell to cell communication in dental biofilm formation (2011)
  • Author(s): Senpuku H
  • Organizer: Istanbul University, Special Seminar
  • Place of Presentation: Istanbul, Turkey(招待講演)
  • Year and Date: 2011-07-01
• [Presentation] Streptococcus mutansにおけるCSP依存新規病原性関連遺伝子の検討 (2011)
  • Author(s): 鈴木奈央未、成澤直規、落合邦康、泉福英信
  • Organizer: 第53回歯科基礎医学会
  • Place of Presentation: 岐阜
• [Presentation] アッサム茶抽出成分によるStreptococcus mutansバイオフィルム阻害に関する研究 (2010)
  • Author(s): 河原井武人、成沢直規、泉福英信
  • Organizer: 第52回歯科基礎医学会
  • Place of Presentation: 千葉
  • Year and Date: 2010-09-21
• [Presentation] う蝕原性Streptococcus mutans smooth-colony-variant出現メカニズム病原性発現に関する検討 (2010)
  • Author(s): 成沢直規、伊藤達朗、鈴木奈央未、佐藤裕、落合邦康、中尾龍馬、泉福英信
  • Organizer: 第52回歯科基礎医学会
  • Place of Presentation: 千葉
  • Year and Date: 2010-09-21
• [Presentation] Effect of polypyrrole on biofilm formation of S.mutans (2010)
  • Author(s): Tuna Elif、前田隆秀、泉福英信
  • Organizer: 第52回歯科基礎医学会
  • Place of Presentation: 千葉
  • Year and Date: 2010-09-21
• [Presentation] Roles od sunL in Streptococcus mutans biofilm formation and aggregation. (2010)
  • Author(s): Senpuku H, Yoneda S, Narisawa N, Kawarai T, Sato Y, Ochiai K, Watanabe H
  • Organizer: 88th general session and exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-03
• [Presentation] Study of dray mouth behavior of E2F-1-deficient NOD/SCID mice (2010)
  • Author(s): Satoh K, Narita T, Matsui-Inohara H, Ito T, Senpuku H, Sugiya H
  • Organizer: 88th general session and exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-03
• [Presentation] Streptococcus mutans colonization on human saliva-treated tooth in NOD/SCID.E2f1 mice. (2010)
  • Author(s): Ito T, Kawarai T, Narisawa N, Zhang X, Kanaguchi N, Maeda T, Senpuku H
  • Organizer: 88th general session and exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-03
• [Presentation] Apperance mechanisms of smooth colony variant in Streptococcus mutans. (2010)
  • Author(s): Narisawa N, Kawarai T, Yoneda S, Ito T, Watanabe H, Senpuku H
  • Organizer: 88th general session and exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-03
• [Presentation] 臨床分離株 Streptococcus mutans の新規バイオフィルム形成遺伝子とその働きについて (2010)
  • Author(s): 米田早織, 成澤直規, 河原井武人, 渡辺治雄, 泉福英信
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 神奈川
  • Year and Date: 2010-03-27
• [Presentation] Streptococcus mutans 自然変異株出現に影響する環境ファクターの探索 (2010)
  • Author(s): 成澤直規, 河原井武人, 米田早織, 渡辺治雄, 泉福英信
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 神奈川
  • Year and Date: 2010-03-27
• [Presentation] Streptococcus mutansの非水溶性グルカン非依存のバイオフィルム形成機序 (2010)
  • Author(s): 河原井武人、成澤直規、米田早織、佐伯洋二、津金貴則、落合邦康、渡辺治雄、泉福英信
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 神奈川
• [Presentation] 臨床分離株Streptococcus mutansの新規バイオフィルム形成遺伝子とその働きについて (2010)
  • Author(s): 米田早織、成澤直規、河原井武人、渡辺治雄、泉福英信
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 神奈川
• [Presentation] Streptococcus mutans自然変異株出現に影響する環境ファクターの探索 (2010)
  • Author(s): 成澤直規、河原井武人、米田早織、渡辺治雄、泉福英信
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 神奈川
• [Presentation] Roles od sunL in Streptococcus mutans biofilm formation and aggregation (2010)
  • Author(s): Senpuku H, Yoneda S, Narisawa N, Kawarai T, Sato Y, Ochiai K, and Watanabe H
  • Organizer: 88th general session and exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
• [Presentation] Study of dray mouth behavior of E2F-1-deficient NOD/SCID mice, Poster Discussion (2010)
  • Author(s): Satoh K, Narita T, Matsui-Inohara H, Ito T, Senpuku H and Sugiya H
  • Organizer: 88th general session and exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
• [Presentation] Streptococcus mutans colonization on human saliva-treated tooth in NOD/SCID. E2f1-/-mice (2010)
  • Author(s): Ito T, Kawarai T, Narisawa N, Zhang X, Kanaguchi N, Maeda T, and Senpuku H
  • Organizer: 88th general session and exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
• [Presentation] Apperance mechanisms of smooth colony variant in Streptococcus mutans (2010)
  • Author(s): Narisawa N, Kawarai T, Yoneda S, Ito T, Watanabe H, and Senpuku H
  • Organizer: 88th general session and exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
• [Presentation] アッサム茶抽出成分によるStreptococcus mutansバイオフィルム阻害に関する研究 (2010)
  • Author(s): 河原井武人、成澤直規、泉福英信
  • Organizer: 第52回歯科基礎医学会
  • Place of Presentation: 東京
• [Presentation] う蝕原性Streptococcus mutans smooth-colony-variant出現メカニズムと病原性発現に関する検討 (2010)
  • Author(s): 成澤直規、伊藤達朗、鈴木奈緒央未、佐藤裕、落合邦康、中尾龍馬、泉福英信
  • Organizer: 第52回歯科基礎医学会
  • Place of Presentation: 東京
• [Presentation] Effect of polypyrole on biofilm formation of S. mutans (2010)
  • Author(s): Tuna Elif,前田隆秀、泉福英信
  • Organizer: 第52回歯科基礎医学会
  • Place of Presentation: 東京
• [Presentation] Establishment of new mouse model using NODISCID.e2f-1^<-1-> mouse for initial adhesion and biofilm formation of Streptococcus mutans (2009)
  • Author(s): Tatsuro Ito, et al.
  • Organizer: 5th ASM Conference on Biofilms
  • Place of Presentation: Cancun, Mexico
  • Year and Date: 2009-11-17
• [Presentation] オートインデューサー制御を利用した複合微生物のバイオフィルム形成について (2009)
  • Author(s): 泉福英信
  • Organizer: 第82回日本生化学会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] オートインデューサー制御を利用した複合微生物のバイオフィルム形成について (2009)
  • Author(s): 泉福英信
  • Organizer: 第82回日本生化学会シンポジウム
  • Place of Presentation: Rome, Italy
  • Year and Date: 2009-10-23
• [Presentation] 高齢者における体力と免疫力;活性化NK細胞との関係 (2009)
  • Author(s): 泉福英信
  • Organizer: 第64回日本体力医学会シンポジウム
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-19
• [Presentation] Streptococcus mutans バイオフィルムの病原性調節遺伝子の解明 (2009)
  • Author(s): 米田早織, 成沢直規, 河原井武人, 泉福英信
  • Organizer: 第51回歯科基礎医学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-11
• [Presentation] Identification of new genes regulated aggregation and biofilm formation of Streptococcus mutans clinical isolates (2009)
  • Author(s): 米田早織, et al.
  • Organizer: Eurobiofilms 2009
  • Place of Presentation: Rome, Italy
  • Year and Date: 2009-09-04
• [Presentation] Study of inhibiting substances from Streptococcus salivarius to Streptococcus mutans biofilm formation (2009)
  • Author(s): Ayako Ogawa, et al.
  • Organizer: Eurobiofilms 2009
  • Place of Presentation: Rome, Italy
  • Year and Date: 2009-09-04
• [Presentation] The effects of naturally-derived variants in virulence expression of Streptococcus mutans (2009)
  • Author(s): 成沢直規, et al.
  • Organizer: Eurobiofilms 2009
  • Place of Presentation: Rome, Italy
  • Year and Date: 2009-09-04
• [Presentation] Water-insoluble glucan-independent biofilm formation by Streptococcus mutans under acidic condition (2009)
  • Author(s): 河原井武人, et al.
  • Organizer: Eurobiofilms 2009
  • Place of Presentation: Rome, Italy
  • Year and Date: 2009-09-04
• [Presentation] Role of salivary IgA antibody against surface protein antigen of Streptococcus mutans in diagnosis and preventive treatment (2009)
  • Author(s): Senpuku H
  • Organizer: 4^<th> Medical Biotech Forum
  • Place of Presentation: Dailian, China
  • Year and Date: 2009-08-10
• [Presentation] Role of salivary IgA antibody against surface protein antigen of Streptococcus mutans in diagnosis and preventive treatment (2009)
  • Author(s): 泉福英信
  • Organizer: 4^<th> Medical Biotech Forum, Dailian
  • Place of Presentation: Dailian, China
  • Year and Date: 2009-08-10
• [Presentation] Identification of new genes regulated aggregation and biofilm formation of Streptococcus mutans clinical isolates (2009)
  • Author(s): Yoneda S, Narisawa N, Kawarai T, Watanabe H, and Senpuku H
  • Organizer: Eurobiofilms 2009
  • Place of Presentation: Italy, Rome
• [Presentation] Study of inhibiting substances from Streptococcus salivarius to Streptococcus mutans biofilm formation (2009)
  • Author(s): Ogawa A, Mitobe J, Fujita S, Kuroda M, Watanabe H, Uematsu H, and Senpuku H
  • Organizer: Eurobiofilms 2009
  • Place of Presentation: Italy, Rome
• [Presentation] The effects of naturally-derived variants in virulence expression of Streptococcus mutans (2009)
  • Author(s): Narisawa N, Kawarai T, Yoneda S, Watanabe H, and Senpuku H
  • Organizer: Eurobiofilms 2009
  • Place of Presentation: Italy, Rome
• [Presentation] Water-insoluble glucan-independent biofilm formation by Streptococcus mutans under acidic condition (2009)
  • Author(s): Kawarai T, Yoneda S, Saeki Y, Tsugane T, Ochiai K, Watanabe H, and Senpuku H
  • Organizer: Eurobiofilms 2009
  • Place of Presentation: Italy, Rome
• [Presentation] Streptococcus mutansバイオフィルムの病原性調節遺伝子の解明 (2009)
  • Author(s): 米田早織、成沢直規、河原井武人、泉福英信
  • Organizer: 第51回歯科基礎医学会
  • Place of Presentation: 新潟
• [Presentation] Establishment of new mouse model using NOD/SCID. e2f-1^<-/-> mouse for initial adhesion and biofilm formation of Streptococcus mutans (2009)
  • Author(s): Ito T, Matsui H, Maeda T, Watanabe H, and Senpuku H
  • Organizer: 5th ASM Conference on Biofilms
  • Place of Presentation: Cancun, Mexico
• [Patent(Industrial Property Rights)] バイオフィルム形成抑制剤 (2010)
  • Inventor(s): 泉福英信、河原井武人、米田早織、堤淑明
  • Industrial Property Rights Holder: 泉福英信、河原井武人、米田早織、堤淑明
  • Filing Date: 2010-08-04
• [Patent(Industrial Property Rights)] バイオフィルム形成抑制剤 (2010)
  • Inventor(s): 泉福英信, 河原井武人, 米田早織, 堤淑明
  • Industrial Property Rights Holder: 泉福英信, 河原井武人, 米田早織, 堤淑明
  • Filing Date: 2010-08-04