| Project/Area Number |
21390508
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
NOIRI Yuichiro 大阪大学, 歯学部附属病院, 講師 (50218286)
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| Co-Investigator(Kenkyū-buntansha) |
EBISU Shigeyuki 大阪大学, 大学院・歯学研究科, 理事・副学長 (50116000)
YABUNE Toshiaki 大阪大学, 大学院・歯学研究科, 助教 (90423144)
ASAHI Yoko 大阪大学, 歯学部附属病院, 医員 (50456943)
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| Co-Investigator(Renkei-kenkyūsha) |
AZAKAMI Hiroyuki 山口大学, 農学部, 准教授 (40263850)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | デンタルバイオフィルム / クオラムセンシング / オートインデューサー / アシルホモセリンラクトン / 感染根管 / マクロライド / アジスロマイシン / Porphyromonas gingivalis / ホモセリンラクトン / LC/MS/MS解析法 / 抗菌剤 / 抗バイオフィルム / レポーターアッセイ / LC / MS / MS解析法 |
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| Research Abstract |
This study was carried out for the establishment of the chemical control and restraint method against human dental biofilm. N-Acyl homoserine lactone(AHL) analogs which was one kind of the autoinducer and was quorum sensing(QS) inducer were targeted. The 10 kinds of AHL analogs were not shown above detection limit(6. 32-7. 33 ng/ ml), and it was clarified that AHL hardly existed in human dental biofilm. On the other hand, We discovered some AHL analogs and antibiotics indicating the anti-biofilm action that would affect QS and made the basics for the clinical application of the chemical biofilm control method by these chemotherapeutic agents.
I take pride in these results of the research giving a significant suggestion for the development of the new treatment strategy of the bacterial biofilm disease.
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