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Evaluation on clarification of transport system of transition metallic ions inside body-defense cells and its association with bio-safety

Research Project

Project/Area Number 21390526
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Dental engineering/Regenerative dentistry
Research InstitutionIwate Medical University

Principal Investigator

TAIRA Masayuki  岩手医科大学, 歯学部, 准教授 (60179398)

Co-Investigator(Kenkyū-buntansha) KAGIYA Tadayoshi  岩手医科大学, 歯学部, 助教 (30405774)
Co-Investigator(Renkei-kenkyūsha) NEZU Akashi  岩手医科大学, 歯学部, 講師 (40264056)
SASAKI Minoru  岩手医科大学, 歯学部, 准教授 (40187133)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Keywords歯学 / 生体材料 / 細胞・組織 / 遷移金属イオン / 遺伝子 / 銅イオンシャペロン / 銅イオントランスポーター / 損傷遺伝子修復酵素
Research Abstract

We cultured macrophage in the medium with copper ions, and evaluated gene expression by quantitative PCR. All expressions of cell-membrane transporter SLC31A1 gene, ER transporter ATP7A/7B genes and copper transport chaperon ATOX1 gene declined when the copper content was larger than 200μmol/L. When high amounts of copper ions were not removed(transported) from the macrophage, it was considered that intracellular excessive copper ions might cause DNA damage(DNA-strand breakage), leading to cell death.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (42 results)

All 2012 2011 2010 2009 Other

All Journal Article (17 results) (of which Peer Reviewed: 16 results) Presentation (19 results) Book (2 results) Remarks (4 results)

  • [Journal Article] Macrophage reaction against sub-micron titanium particles2012

    • Author(s)
      Taira, M., Sasaki, M. and Kimura, S.
    • Journal Title

      Interface Oral Health Science 2011

      Volume: Part6 Pages: 283-284

    • DOI

      10.1007/978-4-431-54070-0_84

    • ISBN
      9784431540694, 9784431540700
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative real-time RT-PCR analyses of DNA-damage. recovery-related gene expressions of mouse macrophage-like cell line RAW264 when exposed to IC50 nickel ions2011

    • Author(s)
      Taira, M., Kagiya, T., Sasaki, M. and Kimura, S.
    • Journal Title

      Nano-Biomedicine

      Volume: 3 Pages: 294-299

    • NAID

      130004555811

    • URL

      https://www.jstage.jst.go.jp/article/nano/3/2/3_2_294/_article

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteome analyses of human macrophages exposed to low cytotoxic IC90 copper(2+) ions2011

    • Author(s)
      Taira M., Shimoyama, Y., Kagiya, T., Sasaki, M., Nezu, T., Harada, H. and Kimura, S.
    • Journal Title

      Dental Mater. J.

      Volume: 30 Pages: 293-299

    • NAID

      10029136627

    • URL

      http://dx.doi.org/10.4012/dmj.2010-088

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteome analyses of human macrophages exposed to low cytotoxic IC90 copper (2+) ions2011

    • Author(s)
      Taira, M., 他6名
    • Journal Title

      Dental Materials Journal

      Volume: 30 Issue: 3 Pages: 293-299

    • DOI

      10.4012/dmj.2010-088

    • NAID

      10029136627

    • ISSN
      0287-4547, 1881-1361
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative real-time RT-PCR analyses of DNA-damage-recovery-related gene expressions of mouse macrophage-like cell line RAW264 when exposed to IC50 nickel ions2011

    • Author(s)
      Taira, M., 他3名
    • Journal Title

      Nano-Biomed

      Volume: 3 Pages: 294-299

    • NAID

      130004555811

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteome analyses of human macrophages exposed to low cytotoxic IC90 Cu(2+) ions2011

    • Author(s)
      Taira, M., Shimoyama Y., Kagiya, T., Sasaki, M., Nezu, T., Harada, H., Kimura, S.
    • Journal Title

      Dental Materials Journal

      Volume: 30(受理済み)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 歯科技工における粉塵について知っておきたいこと2010

    • Author(s)
      平雅之、齋藤設雄
    • Journal Title

      日本歯技

      Volume: 494 Pages: 33-40

    • URL

      http://www.nichigi.or.jp/about_nichigi/nihonshigi.html

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Journal Article] Histological and TEM observation of subcutaneous tissues exposed to particulate copper, nickel and titanium2010

    • Author(s)
      Saitoh, S., Sasaki, K., Nezu, T., Taira, M., Shimoyama, Y., Sasaki, M., Kimura, S. and Ishizeki, K.
    • Journal Title

      J. Oral Tissue Eng.

      Volume: 8 Pages: 102-106

    • NAID

      130004941170

    • URL

      https://www.jstage.jst.go.jp/article/jarde/8/2/8_2_102/_article

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles2010

    • Author(s)
      Taira, M., Kagiya, T., Harada, H., Sasaki, M., 他4名
    • Journal Title

      J. Mater. Sci. Mater. Med.

      Volume: 21 Issue: 1 Pages: 267-275

    • DOI

      10.1007/s10856-009-3834-x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Histological and TEM observation of subcutaneous tissues exposed to particulate copper, nickel and titanium2010

    • Author(s)
      Saitoh, S., Sasaki, K., Nezu, T., Taira, M., Shimoyama, Y., Sasaki, M., Kimura, S., Ishizeki, K
    • Journal Title

      Journal of Oral Tissue Engineering

      Volume: 8 Pages: 102-106

    • NAID

      130004941170

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles2010

    • Author(s)
      Taira, M., Kagiya, T., Harada, H., Sasaki, M., 他4名
    • Journal Title

      J.Mater.Sci.Mater.Med. 21

      Pages: 267-275

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA microarray analyses of the effects of LPS-stimulation and IC50 nickel ions on gene expressions of mouse macrophage-like cell line RAW2642009

    • Author(s)
      Taira, M., Sasaki, M., Sasaki, K., Saitoh, S., Nezu, T., Kimura, S. and Araki, Y.
    • Journal Title

      Nano-Biomedicine

      Volume: 1 Pages: 59-69

    • NAID

      130004555790

    • URL

      https://www.jstage.jst.go.jp/article/nano/1/1/1_1_59/_article

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of aerosols and fine particles produced in dentistry and their health risk assessments2009

    • Author(s)
      Taira, M., Sasaki, M., Kimura, S. and Araki, Y.
    • Journal Title

      Nano-Biomedicine

      Volume: 1 Pages: 9-15

    • NAID

      130004555793

    • URL

      https://www.jstage.jst.go.jp/article/nano/1/1/1_1_9/_article

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] First-stage genome-wide gene expression analyses of human mesenchymal stem cells exposed to IC50 Ni(2+) ions2009

    • Author(s)
      Taira, M., Kagiya, T., Sasaki, M., Sasaki, K., Saitoh, S., 他4名
    • Journal Title

      J. Oral Tissue Eng

      Volume: 7 Pages: 107-120

    • NAID

      130004456605

    • URL

      https://www.jstage.jst.go.jp/article/jarde/7/2/7_2_107/_article

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] First-stage genome-wide gene expression analyses of human mesenchymal stem cells exposed to IC50 Ni(2+) ions2009

    • Author(s)
      Taira, M., Kagiya, T., Sasaki, M., Sasaki, K., Saitoh, S., 他4名
    • Journal Title

      J.Oral Tissue Eng. 7

      Pages: 107-120

    • NAID

      130004456605

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of aerosols and fine particles produced in dentistry and their health risk assessments2009

    • Author(s)
      Taira, M., Sasaki, M., Kimura, S., Araki, Y.
    • Journal Title

      Nano-Bioscience 1

      Pages: 9-15

    • NAID

      130004555793

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA microarray analyses of the effects of LPS-stimulation and IC50 nickel ions on gene expressions of mouse macrophage-like cell line RAW2642009

    • Author(s)
      Taira, M., Sasaki, M., Sasaki, K., Saitoh, S., Nezu, T., Kimura, S., Araki, Y.
    • Journal Title

      Nano-Bioscience 1

      Pages: 59-69

    • NAID

      130004555790

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] THP-1細胞を用いたチタン微粒子、銅イオンとTEGDMAモノマーの細胞障害性の評価2011

    • Author(s)
      平雅之
    • Organizer
      日本動物実験代替法学会第24回大会
    • Place of Presentation
      仙台市
    • Year and Date
      2011-11-12
    • Related Report
      2011 Final Research Report
  • [Presentation] THP-1細胞を用いたチタン微粒子、銅イオンとTEGDMAモノマーの細胞障害性の評価2011

    • Author(s)
      平雅之
    • Organizer
      日本動物実験代替法学会第24回大会
    • Place of Presentation
      仙台市(招待講演)
    • Year and Date
      2011-11-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] EGDMAに曝された時のTHP-1単球の異物代謝並びに炎症性サイトカイン関連遺伝子の発現評価2011

    • Author(s)
      平雅之
    • Organizer
      第58回日本歯科理工学会学術講演会
    • Place of Presentation
      郡山市
    • Year and Date
      2011-10-22
    • Related Report
      2011 Final Research Report
  • [Presentation] TEGDMAに曝された時のTHP-1単球の異物代謝並びに炎症性サイトカイン関連遺伝子の発現評価2011

    • Author(s)
      平雅之
    • Organizer
      第58回日本歯科理工学会学術講演会
    • Place of Presentation
      郡山市
    • Year and Date
      2011-10-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] マクロファージが銅イオンに暴露された時のmicroRNAの発現について2011

    • Author(s)
      鍵谷忠慶、平雅之, 他2名
    • Organizer
      第53回歯科基礎医学会学術大会
    • Place of Presentation
      岐阜市
    • Year and Date
      2011-10-02
    • Related Report
      2011 Final Research Report
  • [Presentation] マクロファージが銅イオンに暴露された時のmicroRNAの発現について2011

    • Author(s)
      鍵谷忠慶, 他3名
    • Organizer
      第54回歯科基礎医学会学術大会
    • Place of Presentation
      岐阜市
    • Year and Date
      2011-10-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] Histological and TEM observation of subcutaneous tissues exposed to particulate pure metals2011

    • Author(s)
      Saitoh, S., Taira, M., 他2名
    • Organizer
      International Dental Materials Congress 2011(IDMC2011)
    • Place of Presentation
      Soul, Korea
    • Year and Date
      2011-05-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Histological and TEM observation of subcutaneous tissues exposed to particulate pure metals2011

    • Author(s)
      Saitoh, S., 他3名
    • Organizer
      International Dental Materials Congress 2011 (IDMC2011)
    • Place of Presentation
      Soul, Korea
    • Year and Date
      2011-05-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 3種類の純金属に対するマウス背部皮下における生体組織反応の評価2010

    • Author(s)
      齋藤設雄, 佐々木かおり, 根津尚史, 平雅之, 下山佑, 佐々木実, 木村重信, 石関清人
    • Organizer
      岩手医科大学歯学会第36回総会
    • Place of Presentation
      盛岡市
    • Year and Date
      2010-12-04
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 低毒性IC90濃度の銅イオンがマクロファージの蛋白発現に及ぼす影響評価2010

    • Author(s)
      平雅之
    • Organizer
      第32回バイオマテリアル学会大会
    • Place of Presentation
      京都市
    • Year and Date
      2010-11-30
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 皮下埋入した金属材料に対する生体組織反応の基礎的評価2010

    • Author(s)
      齋藤設雄, 佐々木かおり, 根津尚史, 平雅之
    • Organizer
      第56回日本歯科理工学会学術講演会
    • Place of Presentation
      岐阜市
    • Year and Date
      2010-10-10
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] マクロファージ内における銅イオン輸送蛋白質の遺伝子発現解析2010

    • Author(s)
      平雅之, 佐々木かおり, 齋藤設雄, 根津尚史
    • Organizer
      第56回日本歯科理工学会学術講演会
    • Place of Presentation
      岐阜市
    • Year and Date
      2010-10-09
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 皮下埋入した金属材料が生体組織に及ぼす影響2010

    • Author(s)
      齋藤設雄, 佐々木かおり, 根津尚史, 平雅之
    • Organizer
      ナノ・バイオメディカル学会第3回大会
    • Place of Presentation
      横浜市
    • Year and Date
      2010-09-17
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 高濃度の銅イオンがマクロファージに及ぼす傷害作用に関する研究2009

    • Author(s)
      平雅之、荒木吉馬
    • Organizer
      第31回バイオマテリアルズ学会大会
    • Place of Presentation
      京都市
    • Year and Date
      2009-11-17
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] IC10%濃度の銅イオンで培養したマクロファージのTEM形態観察と細胞内銅イオン量のEDX測定2009

    • Author(s)
      平雅之、佐々木かおり、齋藤設雄、根津尚史、荒木吉馬
    • Organizer
      第54回日本歯科理工学会学術講演会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2009-10-01
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] IC50ニッケルイオンがヒト間葉系幹細胞の遺伝子発現に及ぼす影響評価2009

    • Author(s)
      平雅之, 佐々木かおり, 他3名
    • Organizer
      第7回日本再生歯科医学会
    • Place of Presentation
      北九州市
    • Year and Date
      2009-09-12
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] IC50濃度ニッケルイオンがLPS活性化マクロファージのDNA傷害修復関連遺伝子の発現に及ぼす影響2009

    • Author(s)
      平雅之、佐々木実、木村重信
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      新潟市
    • Year and Date
      2009-09-10
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] IC50%ニッケルイオンとLPSがマクロファージの全遺伝子発現に及ぼす影響評価2009

    • Author(s)
      平雅之、荒木吉馬
    • Organizer
      ナノ・バイオメディカル学会第1回大会
    • Place of Presentation
      札幌市
    • Year and Date
      2009-07-18
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 銅イオンがマウス多形核白血球(好中球)の活性酸素産生に及ぼす濃度依存的影響2009

    • Author(s)
      平雅之、佐々木かおり、齋藤設雄、根津尚史、荒木吉馬
    • Organizer
      第53回日本歯科理工学会学術講演会
    • Place of Presentation
      東京都
    • Year and Date
      2009-04-11
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Book] 9章ナノ粒子の細胞毒性評価2011

    • Author(s)
      平雅之, 他57名
    • Publisher
      フロンティア出版
    • Related Report
      2011 Final Research Report
  • [Book] ナノ粒子のリスク評価と安全性対策 初版, 亘理文夫 監修 9 ナノ粒子の細胞毒性評価2010

    • Author(s)
      平雅之
    • Total Pages
      6
    • Publisher
      フロンティア出版
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://hitech-d.iwate-med.ac.jp/dmst/index-j.htm

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://hitech-d.iwate-med.ac.jp/dmst/index-j.htm

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://hitech-d.iwata-med.ac.jp/dmst/index-j.htm

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://hitech-d.iwate-med.ac.jp/dmst/index-j.htm

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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