| Project/Area Number |
21390526
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Iwate Medical University |
|---|
Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAGIYA Tadayoshi 岩手医科大学, 歯学部, 助教 (30405774)
|
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| Co-Investigator(Renkei-kenkyūsha) |
NEZU Akashi 岩手医科大学, 歯学部, 講師 (40264056)
SASAKI Minoru 岩手医科大学, 歯学部, 准教授 (40187133)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | 歯学 / 生体材料 / 細胞・組織 / 遷移金属イオン / 遺伝子 / 銅イオンシャペロン / 銅イオントランスポーター / 損傷遺伝子修復酵素 |
|---|
| Research Abstract |
We cultured macrophage in the medium with copper ions, and evaluated gene expression by quantitative PCR. All expressions of cell-membrane transporter SLC31A1 gene, ER transporter ATP7A/7B genes and copper transport chaperon ATOX1 gene declined when the copper content was larger than 200μmol/L. When high amounts of copper ions were not removed(transported) from the macrophage, it was considered that intracellular excessive copper ions might cause DNA damage(DNA-strand breakage), leading to cell death.
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