| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Research Abstract |
I. in vitro analysis The purpose of this study is to reveal the association of autophagy related protein p62 and apoptosis, and carcinogenesis of oral cancer.
FACS analysis revealed that p62 (-/-)MEF increased resitance to apoptosis, and the similar result was obtained from siRNA experiment.
Stat3 and Src phosphorylation were increased in p62 (-/-)MEF. Real time PCR revealed Bcl-2 and Bcl-xL expression were increased. Immuno-blotting analysis showed that Bcl-2, Bcl-xL, and Bax were reduced in p62 (-/-) and wild type MEF by UVB irradiation, however, no significant difference were observed between p62 (-/-) and wild type MEF. ATM mRNA expression were not different between p62 (-/-) and wild type MEF.
II. in vivo analysis By UVB irradiation to skin of p62 (-/-) and wild typ mice showed that p62 (-/-) skin had less apoptotic response resulting resistance to apoptosis.
III. Additional results Prx I (-/-) MEF showed reduced resistance to apoptosis. We found NASH model mouse by using p62 (-/-) and Nrf2 (-/-) mice.
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