Isolation of molecules synthesized by stromal cells, which regulate tumor invasion and metastasis : application in diagnosis and therapy

• Project/Area Number: 21390538
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: HAYASHIDO Yasutaka 広島大学, 病院, 講師 (70243251)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Tetsuji 広島大学, 医歯薬学総合研究科, 教授 (00169153) ; 新谷 智章 広島大学, 病院, 助教 (90403518)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2009: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
• Keywords: 口腔癌 / 浸潤・転移 / 間質細胞 / 細胞増殖 / 細胞遊走 / 蛋白分解活性 / インテグリン / 線維芽細胞 / 細胞接着因子

Research Abstract

To assess the participation of stromal components in the progression of oral squamous cell carcinoma(SCC) cells, we investigated the effect of fibroblasts on the proliferation, motility and proteolytic activity of SCC cells. Fibroblasts in the stroma of oral SCC synthesize TSP-1, which induces haptotactic migration and enhances the ability to synthesize matrix metallopeptidase 9(MMP-9) in oral SCC cells. Further type I collagen produced by fibroblasts facilitates the proliferation and migration of oral SCC cells via integrinαvβ8

Research Products

• [Journal Article] 広島大学病院顎・口腔外科における過去10年間の顎顔面骨骨折の臨床統計的検討 (2011)
  • Author(s): 坂上泰士, 福井康人, 他
  • Journal Title: 広島大学歯学雑誌 Volume: 43(1) Pages: 20-26
  • Peer Reviewed
• [Journal Article] Advantages and difficulties in culturing human pluripotent stem cells in growth factor-defined serum-free medium (2010)
  • Author(s): Kusuda Furue M, Tateyama D, Kinehara M, Na J, Okamoto T, Sato JD
  • Journal Title: InVitro Cell Dev Biol Anim Volume: 46(7) Pages: 573-576
  • Peer Reviewed
• [Journal Article] Binding of APC and dishevelled mediates Wnt5a-regulated focal adhesion dynamics in migrating cells (2010)
  • Author(s): Matsumoto S, Fumoto K, Okamoto T, Kaibuchi K, Kikuchi A
  • Journal Title: EMBO J Volume: 29(7) Pages: 1192-1204
  • Peer Reviewed
• [Journal Article] 偽悪性変化を伴った下顎歯肉腫瘤の1例 (2010)
  • Author(s): 吉岡幸男, 小川郁子, 林堂安貴, 虎谷茂昭, 高田隆, 岡本哲治
  • Journal Title: 日本口腔科学会雑誌 Volume: 42 Pages: 63-67
  • NAID: 10027093100
  • Peer Reviewed
• [Journal Article] ESR dosimetry study on population of settlements nearby Ust-Kamenogorsk city (2009)
  • Author(s): Zhumadilov K, Ivannikov A, ZharlyganovaD, Zhumadilov Z, Stepanenko V, Apsalikov K, Ali MR, Zhumadilova A, Toyoda S, Endo S, Tanaka K, Okamoto T, Hoshi M
  • Journal Title: Kazakhstan. Radiat Environ Biophys Volume: 48(4) Pages: 419-425
  • Peer Reviewed
• [Journal Article] オトガイ部知覚異常を初発症状とした側頭下窩に進展した筋肉内脂肪腫の1例 (2009)
  • Author(s): 福井康人, 林堂安貴, 他
  • Journal Title: 日本口腔外科学会雑誌 55 Pages: 580-584
  • NAID: 10027091398
  • Peer Reviewed
• [Presentation] 口腔扁平上皮癌細胞におけるユビキチン・プロテアソーム系によるインテグリンβ8の翻訳後修飾 (2011)
  • Author(s): 浜名智昭, 林堂安貴, 福井康人, 白砂兼光, 岡本哲治
  • Organizer: 第48回日本口腔組織培養学会学術大会
  • Place of Presentation: 浦安
  • Year and Date: 2011-11-19
• [Presentation] ユビキチンプロテアソーム系による口腔扁平上皮癌細胞のインテグリンβ8の翻訳後修飾 (2011)
  • Author(s): 浜名智昭, 林堂安貴, 有田裕一, 白砂兼光, 岡本哲治
  • Organizer: 第56回(社)日本口腔外科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-22
• [Presentation] The processingintegrinβ8 in oral squamous cellcarcinoma cells byubiquitin-proteasome system (2011)
  • Author(s): Tomoaki HAMANA, Yasutaka HAYASHIDO, Yasuto FUKUI, Kanemitsu SHIRASUNA, Tetsuji OKAMOTO
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-08-09
• [Presentation] The processing integrin β8 in oral squamous cell carcinoma cells by ubiquitin-proteasome system (2011)
  • Author(s): Tomoaki HAMANA, Yasutaka HAYASHIDO, Yasuto FUKUI, Kanemitsu SHIRASUNA, Tetsuji OKAMOTO
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-08-09
• [Presentation] 口腔扁平上皮癌細胞におけるユビキチン・プロテアソーム系によるインテグリンβ8のプロセシングについての解析 (2011)
  • Author(s): 浜名智昭, 林堂安貴, 有田裕一, 白砂兼光, 岡本哲治
  • Organizer: 第65回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-04-22
• [Presentation] 唾液中のheparin-binding protein 17(HBp17)/ FGF-binding protein(FGFBP)の同定 (2010)
  • Author(s): 新谷智章, 林堂安貴, 藤井良典, 笛吹恵美子, 虎谷茂昭, 岡本哲治
  • Organizer: 第64回日本口腔科学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-25
• [Presentation] FGF結合蛋白FGFBP/HBp17を標的としたRNAiによる口腔扁平上皮癌細胞の増殖抑制効果の検討 (2010)
  • Author(s): 岡崎文彦, 新谷智章, 林堂安貴, 藤井良典, 笛吹恵美子, 虎谷茂昭, 岡本哲治
  • Organizer: 第64回 NPO法人 日本口腔科学会学術集会
  • Place of Presentation: 札幌市
  • Year and Date: 2010-06-24
• [Book] 口腔外科学第3版 (2010)
  • Author(s): 林堂安貴,岡本哲治
  • Publisher: 医歯薬出版
• [Book] 口腔外科学(第3版) (2010)
  • Author(s): 林堂安貴, 他, 白砂兼光, 古郷幹彦編
  • Total Pages: 815
  • Publisher: 医歯薬出版